Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TEMA_RS15310 Genome accession   NZ_CP101637
Coordinates   3044594..3045655 (+) Length   353 a.a.
NCBI ID   WP_228105568.1    Uniprot ID   -
Organism   Terrisporobacter mayombei strain DSM 6539     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3039594..3050655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TEMA_RS15295 (TEMA_31600) tyrS 3040418..3041641 (+) 1224 WP_228105626.1 tyrosine--tRNA ligase -
  TEMA_RS15300 (TEMA_31610) glmU 3041854..3043230 (+) 1377 WP_228105570.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
  TEMA_RS15305 (TEMA_31620) - 3043291..3044241 (+) 951 WP_228105569.1 ribose-phosphate diphosphokinase -
  TEMA_RS15310 (TEMA_31630) pilT 3044594..3045655 (+) 1062 WP_228105568.1 type IV pilus twitching motility protein PilT Machinery gene
  TEMA_RS15315 (TEMA_31640) - 3045966..3046610 (+) 645 WP_309164819.1 A24 family peptidase -
  TEMA_RS15320 (TEMA_31650) pth 3046623..3047186 (+) 564 WP_228105566.1 aminoacyl-tRNA hydrolase -
  TEMA_RS15325 (TEMA_31660) mfd 3047200..3050592 (+) 3393 WP_228105565.1 transcription-repair coupling factor -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 39748.26 Da        Isoelectric Point: 7.2863

>NTDB_id=711084 TEMA_RS15310 WP_228105568.1 3044594..3045655(+) (pilT) [Terrisporobacter mayombei strain DSM 6539]
MNIFDILRAAIELEASDIHITVGSVPVARIKGRFTKLTENILTKHDTEDMTKKIAGDKNFKKIEEHGECDFSVSIESGER
FRVNAYKQKGNYAIAIRTITSQIPSFETLGLPEILKTFTEKHKGLVLVTGPTGSGKSTTLASLIDIINKNQQRHIITLED
PIEYVHNHKKSLVNQREIGQDTESFNSALRAILRQDPDVILVGEMRDSETISIALTAAETGHLVFSTLHTVGAAKTIDRI
VDMFPSEQQHQIRTQLSTVCEGVISQQLLQTIDERNRVAALEIMVSNPAIRNLIRENKTYQIQNIIQTGSKHGMQSMDQE
LVNLYRQGRISRQSVLSRCTDYEYTSRLVGDRY

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=711084 TEMA_RS15310 WP_228105568.1 3044594..3045655(+) (pilT) [Terrisporobacter mayombei strain DSM 6539]
ATGAATATATTTGATATATTAAGAGCAGCAATAGAACTTGAAGCATCAGATATACATATTACAGTAGGTAGTGTACCAGT
AGCTAGGATAAAAGGAAGATTTACAAAATTAACAGAAAATATATTAACAAAGCATGATACAGAAGATATGACAAAAAAAA
TTGCTGGAGATAAAAATTTTAAAAAAATTGAAGAACATGGTGAATGTGATTTTTCTGTATCTATAGAAAGTGGAGAAAGG
TTTAGGGTAAATGCATATAAGCAAAAAGGAAATTATGCTATTGCAATAAGAACCATTACTTCTCAGATTCCTTCCTTCGA
AACCCTGGGACTACCGGAGATTCTTAAAACTTTTACAGAAAAACATAAAGGCTTGGTGTTAGTTACAGGACCAACAGGAA
GTGGTAAAAGTACAACTCTTGCAAGTCTTATAGACATAATAAATAAAAATCAACAAAGACATATTATAACTTTGGAAGAT
CCTATAGAATATGTTCATAATCATAAGAAAAGCTTAGTAAATCAAAGGGAAATAGGACAAGATACTGAAAGCTTTAACTC
TGCTCTAAGAGCAATACTTCGTCAAGATCCTGATGTTATTCTTGTAGGGGAAATGAGAGACTCAGAAACGATTTCTATAG
CATTAACAGCAGCGGAGACAGGTCATTTAGTATTTTCTACTCTTCACACCGTTGGAGCAGCCAAAACCATAGACCGCATA
GTAGATATGTTTCCATCAGAGCAACAACATCAGATTAGAACACAGTTATCGACTGTATGTGAAGGTGTTATTTCTCAACA
GTTATTACAGACTATAGATGAAAGAAATAGAGTAGCAGCCTTAGAAATTATGGTATCAAACCCAGCAATAAGGAATTTGA
TAAGAGAAAATAAAACTTATCAAATTCAAAATATTATACAAACAGGAAGCAAGCACGGTATGCAATCTATGGATCAAGAA
TTAGTTAATTTATATAGACAAGGAAGGATATCAAGACAGAGTGTACTTAGTAGATGTACGGATTATGAATACACTAGTAG
ATTAGTTGGAGATAGATACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50.289

98.017

0.493
  pilT Neisseria gonorrhoeae MS11

50

98.017

0.49
  pilT Legionella pneumophila strain ERS1305867

51.198

94.618

0.484
  pilT Legionella pneumophila strain Lp02

51.198

94.618

0.484
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.718

99.433

0.484
  pilT Acinetobacter baumannii strain A118

50.898

94.618

0.482
  pilT Acinetobacter nosocomialis M2

50.898

94.618

0.482
  pilT Acinetobacter baumannii D1279779

50.898

94.618

0.482
  pilT Vibrio cholerae strain A1552

50

95.751

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

95.751

0.479
  pilT Pseudomonas stutzeri DSM 10701

50

94.618

0.473
  pilT Pseudomonas aeruginosa PAK

50

94.618

0.473
  pilT Acinetobacter baylyi ADP1

50.151

93.768

0.47
  pilU Acinetobacter baylyi ADP1

40

96.317

0.385
  pilU Vibrio cholerae strain A1552

39.881

95.184

0.38
  pilU Pseudomonas stutzeri DSM 10701

38.393

95.184

0.365