Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NOG67_RS03820 Genome accession   NZ_CP101613
Coordinates   832421..833416 (+) Length   331 a.a.
NCBI ID   WP_062744322.1    Uniprot ID   A0A3S7TG93
Organism   Erwinia persicina strain SR15     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 827421..838416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NOG67_RS03790 (NOG67_03785) - 827906..828232 (+) 327 WP_062744328.1 YggL family protein -
  NOG67_RS03795 (NOG67_03790) - 828382..829095 (+) 714 WP_137269038.1 DUF2884 domain-containing protein -
  NOG67_RS03800 (NOG67_03795) hemW 829194..830330 (-) 1137 WP_062744326.1 radical SAM family heme chaperone HemW -
  NOG67_RS03805 (NOG67_03800) rdgB 830323..830916 (-) 594 WP_062744325.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  NOG67_RS03810 (NOG67_03805) - 831144..831683 (-) 540 WP_062744324.1 YggT family protein -
  NOG67_RS03815 (NOG67_03810) - 831692..832405 (-) 714 WP_137269037.1 YggS family pyridoxal phosphate-dependent enzyme -
  NOG67_RS03820 (NOG67_03815) pilT 832421..833416 (+) 996 WP_062744322.1 type IV pilus twitching motility protein PilT Machinery gene
  NOG67_RS03825 (NOG67_03820) ruvX 833532..833954 (-) 423 WP_174522416.1 Holliday junction resolvase RuvX -
  NOG67_RS03830 (NOG67_03825) - 833954..834517 (-) 564 WP_062744320.1 YqgE/AlgH family protein -
  NOG67_RS03835 (NOG67_03830) gshB 834608..835552 (-) 945 WP_255851436.1 glutathione synthase -
  NOG67_RS03840 (NOG67_03835) rsmE 835619..836350 (-) 732 WP_062744318.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NOG67_RS03845 (NOG67_03840) endA 836404..837117 (-) 714 WP_255851437.1 deoxyribonuclease I -
  NOG67_RS03850 (NOG67_03845) - 837239..837751 (-) 513 WP_137269036.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 331 a.a.        Molecular weight: 36116.44 Da        Isoelectric Point: 6.1221

>NTDB_id=710898 NOG67_RS03820 WP_062744322.1 832421..833416(+) (pilT) [Erwinia persicina strain SR15]
MDIEELVALSVKHNAGDLHLCSGHSPFWRCNGRLAPIEGMPEMTNEWLESFAVRWLTVVQQQTLAEEGQVDFALTMPDGV
RLRASLFRQRHGLSLALRLIASRCPDMTSLLLPAVTQSLLLREEGLILIVGATGSGKSTTLAAMIDGMNQQQQRHILTLE
DPVEYLHHSEQSLIQQREKGLHFTDFGGGLRAALREDPDVILLGELRDAETIQLALTAAETGHLVLATLHTRGAAQAVER
LVDVFPGNEKNLVRSQLAGSLQAVIAQRLVATTGGRVALFEVLVNSPAVANLIREGKTHQLPALLQTGSQQGMQTFEQSL
AERQRMGVLAG

Nucleotide


Download         Length: 996 bp        

>NTDB_id=710898 NOG67_RS03820 WP_062744322.1 832421..833416(+) (pilT) [Erwinia persicina strain SR15]
ATGGATATTGAGGAATTAGTGGCCCTTAGTGTAAAGCATAATGCAGGCGATCTGCACCTTTGCAGTGGTCATTCTCCATT
CTGGCGCTGCAATGGCCGCCTGGCACCGATTGAGGGTATGCCGGAGATGACCAATGAGTGGCTGGAAAGCTTCGCTGTAC
GCTGGCTGACGGTCGTTCAGCAACAGACGCTGGCAGAGGAAGGGCAGGTGGACTTTGCCCTCACGATGCCGGACGGCGTG
CGCCTGCGGGCCAGTCTTTTTCGTCAGCGCCACGGTTTATCGCTGGCGCTGCGCCTGATTGCCAGCCGCTGTCCGGATAT
GACGTCACTGCTTCTTCCTGCGGTGACCCAATCGCTGCTGCTGCGGGAAGAGGGCCTGATTCTGATCGTCGGCGCTACCG
GTAGCGGAAAATCCACTACGCTGGCGGCAATGATTGACGGGATGAACCAGCAGCAGCAGCGCCATATTCTGACGCTGGAA
GATCCGGTTGAATATCTGCATCACAGCGAGCAGTCGCTGATCCAGCAGCGTGAGAAAGGGCTGCATTTTACCGACTTTGG
CGGAGGGCTGAGGGCGGCGCTGAGGGAAGACCCTGATGTGATACTGCTGGGGGAACTGCGCGACGCCGAGACGATTCAAC
TGGCGCTGACTGCCGCCGAAACGGGGCATCTGGTGCTGGCAACGCTGCATACCCGAGGGGCGGCGCAGGCGGTGGAGCGA
CTGGTGGATGTCTTTCCGGGAAACGAAAAGAATCTGGTACGCAGCCAACTGGCGGGCAGCTTACAGGCGGTGATTGCCCA
GCGACTGGTAGCAACCACTGGCGGCAGGGTCGCGCTGTTCGAGGTACTGGTGAACAGTCCGGCAGTGGCTAACCTGATCC
GCGAGGGGAAAACCCATCAGTTGCCTGCACTCTTGCAGACTGGCAGTCAGCAGGGGATGCAGACGTTTGAGCAAAGCCTG
GCGGAAAGGCAGAGAATGGGGGTTCTGGCTGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(41-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S7TG93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

48.494

100

0.486
  pilT Neisseria gonorrhoeae MS11

48.193

100

0.483
  pilT Vibrio cholerae strain A1552

46.97

99.698

0.468
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

46.97

99.698

0.468
  pilT Acinetobacter baumannii D1279779

45.015

100

0.45
  pilT Acinetobacter nosocomialis M2

45.015

100

0.45
  pilT Acinetobacter baumannii strain A118

45.015

100

0.45
  pilT Pseudomonas stutzeri DSM 10701

46.417

96.979

0.45
  pilT Acinetobacter baylyi ADP1

44.848

99.698

0.447
  pilT Pseudomonas aeruginosa PAK

45.483

96.979

0.441
  pilT Legionella pneumophila strain Lp02

45.201

97.583

0.441
  pilT Legionella pneumophila strain ERS1305867

45.201

97.583

0.441
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.176

97.583

0.402
  pilU Pseudomonas stutzeri DSM 10701

40.308

98.187

0.396
  pilU Vibrio cholerae strain A1552

38.369

100

0.384
  pilU Acinetobacter baylyi ADP1

37.5

99.094

0.372