NTDB

Overview

Name	pilA2	Type	Machinery gene
Locus tag	NMB96_RS23230	Genome accession	NZ_CP101417
Coordinates	5420650..5421075 (-)	Length	141 a.a.
NCBI ID	WP_023904908.1	Uniprot ID	A0A6V7CJ00
Organism	Xanthomonas hortorum strain jj2001
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5415650..5426075

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NMB96_RS23210 (NMB96_23185)	pilR	5416334..5417782 (+)	1449	WP_181018098.1	sigma-54 dependent transcriptional regulator	Regulator
NMB96_RS23215 (NMB96_23190)	-	5417856..5418038 (+)	183	WP_074066024.1	YhfG family protein	-
NMB96_RS23220 (NMB96_23195)	-	5418035..5418652 (+)	618	WP_255085842.1	Fic family protein	-
NMB96_RS23225 (NMB96_23200)	pilB	5418661..5420397 (-)	1737	WP_023904907.1	type IV-A pilus assembly ATPase PilB	Machinery gene
NMB96_RS23230 (NMB96_23205)	pilA2	5420650..5421075 (-)	426	WP_023904908.1	pilin	Machinery gene
NMB96_RS23235 (NMB96_23210)	pilC	5421428..5422687 (+)	1260	WP_023904909.1	type II secretion system F family protein	Machinery gene
NMB96_RS23240 (NMB96_23215)	-	5422694..5423557 (+)	864	WP_002812278.1	A24 family peptidase	-
NMB96_RS23245 (NMB96_23220)	coaE	5423571..5424194 (+)	624	WP_023904910.1	dephospho-CoA kinase	-
NMB96_RS23250 (NMB96_23225)	-	5424397..5425377 (+)	981	WP_183086603.1	IS5 family transposase	-

Sequence

Protein

Download Length: 141 a.a. Molecular weight: 14364.59 Da Isoelectric Point: 9.0168

                                >NTDB_id=710107 NMB96_RS23230 WP_023904908.1 5420650..5421075(-) (pilA2) [Xanthomonas hortorum strain jj2001]

MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYLVKARVSEVMLAASSARTAVSEAAAADTSATNMPATFTVETQASKYV

ASVAYVGGATGVITATAKDLGGSTPTGTITLTGTKAANGQVSWVCAGTIDVKYRPATCRGT

Nucleotide

Download Length: 426 bp

                                >NTDB_id=710107 NMB96_RS23230 WP_023904908.1 5420650..5421075(-) (pilA2) [Xanthomonas hortorum strain jj2001]

ATGAAGAAGCAACAAGGTTTTACCCTGATCGAATTGATGATTGTGGTTGCGATCATCGCAATTCTGGCTGCCATCGCACT

GCCGGCTTATCAGGATTACCTGGTCAAGGCGCGCGTTTCGGAAGTGATGCTGGCCGCGTCTTCGGCACGCACGGCAGTTT

CGGAAGCTGCTGCTGCCGATACCAGTGCTACTAATATGCCCGCCACCTTTACTGTCGAGACACAGGCATCCAAGTATGTG

GCGAGTGTCGCCTATGTGGGTGGCGCCACGGGTGTCATCACTGCAACCGCCAAGGATTTGGGTGGCAGTACTCCTACTGG

AACAATTACACTAACTGGTACTAAGGCAGCCAACGGTCAGGTCTCCTGGGTGTGTGCTGGTACCATCGATGTGAAGTATC

GTCCTGCTACCTGTCGCGGTACCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-139)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A6V7CJ00

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA2	Legionella pneumophila str. Paris	53.521	100	0.539
pilA2	Legionella pneumophila strain ERS1305867	52.817	100	0.532
comP	Acinetobacter baylyi ADP1	47.712	100	0.518
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	38.919	100	0.511
pilA	Ralstonia pseudosolanacearum GMI1000	40	100	0.468
pilE	Neisseria gonorrhoeae MS11	37.654	100	0.433
pilA	Vibrio campbellii strain DS40M4	44.776	95.035	0.426
pilA/pilA1	Eikenella corrodens VA1	38.312	100	0.418
pilE	Neisseria gonorrhoeae strain FA1090	36.875	100	0.418
pilA/pilAI	Pseudomonas stutzeri DSM 10701	41.007	98.582	0.404
pilA	Pseudomonas aeruginosa PAK	35.526	100	0.383
pilA	Acinetobacter baumannii strain A118	36.62	100	0.369
pilA	Glaesserella parasuis strain SC1401	35.616	100	0.369
pilA/pilAII	Pseudomonas stutzeri DSM 10701	36.957	97.872	0.362

Detailed information NTG0710107