Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMK62_RS04215 Genome accession   NZ_CP101310
Coordinates   838982..839962 (+) Length   326 a.a.
NCBI ID   WP_022581223.1    Uniprot ID   A0A9Q6Y405
Organism   Escherichia coli strain 99-3311     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 833982..844962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMK62_RS04185 (NMK62_04185) yggM 834583..835590 (+) 1008 WP_000745257.1 DUF1202 family protein -
  NMK62_RS04190 (NMK62_04190) hemW 835659..836795 (-) 1137 WP_000239930.1 radical SAM family heme chaperone HemW -
  NMK62_RS04195 (NMK62_04195) rdgB 836788..837381 (-) 594 WP_001174746.1 XTP/dITP diphosphatase -
  NMK62_RS04200 (NMK62_04200) yggU 837389..837679 (-) 291 WP_001277219.1 DUF167 family protein YggU -
  NMK62_RS04205 (NMK62_04205) yggT 837676..838242 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  NMK62_RS04210 (NMK62_04210) yggS 838260..838964 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  NMK62_RS04215 (NMK62_04215) pilT 838982..839962 (+) 981 WP_022581223.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMK62_RS04220 (NMK62_04220) ruvX 840153..840569 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  NMK62_RS04225 (NMK62_04225) yqgE 840569..841132 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  NMK62_RS04230 (NMK62_04230) gshB 841241..842191 (-) 951 WP_000593289.1 glutathione synthase -
  NMK62_RS04235 (NMK62_04235) rsmE 842204..842935 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NMK62_RS04240 (NMK62_04240) endA 843015..843722 (-) 708 WP_000286503.1 deoxyribonuclease I -
  NMK62_RS04245 (NMK62_04245) yggI 843817..844314 (-) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35840.17 Da        Isoelectric Point: 6.0974

>NTDB_id=709597 NMK62_RS04215 WP_022581223.1 838982..839962(+) (pilT) [Escherichia coli strain 99-3311]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLKNGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSCCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLNCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVKRLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=709597 NMK62_RS04215 WP_022581223.1 838982..839962(+) (pilT) [Escherichia coli strain 99-3311]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCACCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGAAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGTTGTTACCTTCGTGCTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGTAAAT
CCACCACGCTGGCGGCAATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTGAATTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCGGCGCAGGCAGTTAAGCGGCTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCAGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

36.728

99.387

0.365