NTDB

Overview

Name	pilT	Type	Machinery gene
Locus tag	NLG07_RS05525	Genome accession	NZ_CP101291
Coordinates	1180033..1181076 (+)	Length	347 a.a.
NCBI ID	WP_254856694.1	Uniprot ID	-
Organism	Alteromonas sp. LMIT006
Function	mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1175033..1186076

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NLG07_RS05495 (NLG07_05495)	yaaA	1176105..1176875 (+)	771	WP_254856688.1	peroxide stress protein YaaA	-
NLG07_RS05500 (NLG07_05500)	-	1177175..1177381 (-)	207	WP_254856689.1	hypothetical protein	-
NLG07_RS05505 (NLG07_05505)	-	1177462..1177881 (-)	420	WP_254856690.1	DUF4426 domain-containing protein	-
NLG07_RS05510 (NLG07_05510)	-	1177920..1178462 (-)	543	WP_254856691.1	YggT family protein	-
NLG07_RS05515 (NLG07_05515)	proC	1178471..1179292 (-)	822	WP_254856692.1	pyrroline-5-carboxylate reductase	-
NLG07_RS05520 (NLG07_05520)	-	1179306..1180007 (-)	702	WP_254856693.1	YggS family pyridoxal phosphate-dependent enzyme	-
NLG07_RS05525 (NLG07_05525)	pilT	1180033..1181076 (+)	1044	WP_254856694.1	type IV pilus twitching motility protein PilT	Machinery gene
NLG07_RS05530 (NLG07_05530)	-	1181185..1181496 (+)	312	WP_254856695.1	DUF2007 domain-containing protein	-
NLG07_RS05535 (NLG07_05535)	ruvX	1181508..1181927 (-)	420	WP_254856696.1	Holliday junction resolvase RuvX	-
NLG07_RS05540 (NLG07_05540)	-	1181924..1182478 (-)	555	WP_254856697.1	YqgE/AlgH family protein	-
NLG07_RS05545 (NLG07_05545)	gshB	1182493..1183440 (-)	948	WP_254856698.1	glutathione synthase	-
NLG07_RS05550 (NLG07_05550)	-	1183437..1184168 (-)	732	WP_254856699.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
NLG07_RS05555 (NLG07_05555)	-	1184540..1185355 (+)	816	WP_254856818.1	mechanosensitive ion channel family protein	-

Sequence

Protein

Download Length: 347 a.a. Molecular weight: 38448.97 Da Isoelectric Point: 6.2833

                                >NTDB_id=709520 NLG07_RS05525 WP_254856694.1 1180033..1181076(+) (pilT) [Alteromonas sp. LMIT006]

MDITELLAFSVKNNASDLHLSAGQPPMNRVDGEMRRLNVPALDHKVVQALIYEVMNDQQRCEFEENFEVDFSFEVKGLAR

FRVNVFGQNRGCAAVLRTIPSKVLTLDEIGAPNIFRDIIDQPTGIVLVTGATGSGKSTTLAAMIDHINTHKREHILTIED

PIEFVHDNKLCLINQREVHRDTKSFSNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI

IDVFPAEEKSMVRSMLSESLRAVISQNLLKRQGGGRVAAHEIMLGVPAIRNLIREDKVPQMYSVMQTGQGHGMQTMAQCV

QRLVAQGLVSSTDAQEVFSDGKNSVNF

Nucleotide

Download Length: 1044 bp

                                >NTDB_id=709520 NLG07_RS05525 WP_254856694.1 1180033..1181076(+) (pilT) [Alteromonas sp. LMIT006]

ATGGATATTACCGAATTACTTGCCTTTAGTGTTAAGAATAACGCATCGGACCTGCATTTGTCAGCAGGGCAGCCTCCAAT

GAACCGCGTCGATGGTGAAATGCGTCGCCTCAATGTGCCCGCTCTTGACCATAAAGTGGTACAGGCTCTTATTTACGAGG

TAATGAATGACCAGCAACGCTGCGAATTCGAAGAAAATTTCGAAGTTGATTTCTCCTTTGAAGTCAAAGGATTAGCTCGT

TTTAGGGTCAATGTATTTGGTCAAAACCGAGGTTGTGCCGCCGTTTTACGAACCATACCGAGTAAAGTCTTAACTCTGGA

TGAAATCGGTGCACCAAATATTTTTAGAGATATTATTGACCAACCGACAGGAATTGTTTTGGTCACAGGAGCAACAGGCT

CAGGTAAATCCACCACCCTTGCGGCGATGATCGATCACATTAATACCCACAAGCGCGAGCATATATTGACCATTGAAGAT

CCGATTGAATTTGTGCATGACAACAAACTATGTTTGATCAATCAGCGCGAAGTGCATCGTGATACCAAATCGTTTTCTAA

CGCATTGCGTTCAGCATTACGTGAAGATCCAGATGTGATTTTGGTTGGTGAGTTACGTGACCTAGAAACGATTCGCTTGG

CCATATCCGCAGCAGAAACTGGGCATCTTGTGTTTGGCACCTTGCACACCAACTCCGCACCCAAAACCATAGATAGAATT

ATTGATGTATTTCCGGCAGAAGAAAAATCGATGGTGCGCTCTATGCTTTCGGAAAGTTTACGCGCGGTGATTTCACAAAA

CCTGTTAAAACGCCAAGGGGGAGGCAGGGTTGCTGCACACGAAATCATGCTAGGCGTACCTGCGATCCGCAATTTGATTC

GCGAAGATAAAGTACCGCAGATGTACTCAGTCATGCAAACGGGGCAAGGACATGGTATGCAAACGATGGCTCAATGTGTA

CAGCGTCTTGTGGCACAAGGTTTAGTCTCCTCGACTGATGCTCAGGAAGTCTTTTCAGACGGTAAAAATTCAGTGAATTT

CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilT	Acinetobacter baylyi ADP1	73.591	97.118	0.715
pilT	Pseudomonas stutzeri DSM 10701	73.512	96.83	0.712
pilT	Acinetobacter baumannii D1279779	73.134	96.542	0.706
pilT	Acinetobacter baumannii strain A118	73.134	96.542	0.706
pilT	Acinetobacter nosocomialis M2	72.836	96.542	0.703
pilT	Pseudomonas aeruginosa PAK	72.619	96.83	0.703
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	71.681	97.695	0.7
pilT	Vibrio cholerae strain A1552	71.681	97.695	0.7
pilT	Legionella pneumophila strain Lp02	69.048	96.83	0.669
pilT	Legionella pneumophila strain ERS1305867	69.048	96.83	0.669
pilT	Neisseria gonorrhoeae MS11	65.774	96.83	0.637
pilT	Neisseria meningitidis 8013	66.767	95.389	0.637
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	53.061	98.847	0.524
pilU	Vibrio cholerae strain A1552	41.194	96.542	0.398
pilU	Pseudomonas stutzeri DSM 10701	40.597	96.542	0.392
pilU	Acinetobacter baylyi ADP1	40.476	96.83	0.392

Detailed information NTG0709520