Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NK667_RS29490 Genome accession   NZ_CP101125
Coordinates   6675921..6676955 (+) Length   344 a.a.
NCBI ID   WP_054616926.1    Uniprot ID   -
Organism   Pseudomonas nunensis strain In5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6670921..6681955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NK667_RS29460 (NK667_29460) - 6671135..6671569 (-) 435 WP_054044419.1 DUF4426 domain-containing protein -
  NK667_RS29465 (NK667_29465) metW 6671596..6672216 (-) 621 WP_054044417.1 methionine biosynthesis protein MetW -
  NK667_RS29470 (NK667_29470) metX 6672224..6673363 (-) 1140 WP_054044416.1 homoserine O-succinyltransferase MetX -
  NK667_RS29475 (NK667_29475) - 6673561..6674148 (-) 588 WP_054044414.1 YggT family protein -
  NK667_RS29480 (NK667_29480) proC 6674173..6674991 (-) 819 WP_054616928.1 pyrroline-5-carboxylate reductase -
  NK667_RS29485 (NK667_29485) - 6675027..6675725 (-) 699 WP_054616927.1 YggS family pyridoxal phosphate-dependent enzyme -
  NK667_RS29490 (NK667_29490) pilT 6675921..6676955 (+) 1035 WP_054616926.1 type IV pilus twitching motility protein PilT Machinery gene
  NK667_RS29495 (NK667_29495) - 6677085..6677705 (-) 621 WP_054044406.1 C40 family peptidase -
  NK667_RS29500 (NK667_29500) - 6677956..6678390 (+) 435 WP_054044405.1 NINE protein -
  NK667_RS29505 (NK667_29505) - 6678495..6679766 (-) 1272 WP_054616925.1 dihydroorotase -
  NK667_RS29510 (NK667_29510) - 6679763..6680767 (-) 1005 WP_007897943.1 aspartate carbamoyltransferase catalytic subunit -
  NK667_RS29515 (NK667_29515) pyrR 6680795..6681301 (-) 507 WP_054044401.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  NK667_RS29520 (NK667_29520) ruvX 6681383..6681820 (-) 438 WP_005792259.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38097.73 Da        Isoelectric Point: 7.0159

>NTDB_id=708912 NK667_RS29490 WP_054616926.1 6675921..6676955(+) (pilT) [Pseudomonas nunensis strain In5]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMDDLGMGEVFRKITEAPRGLVLVTGPTGSGKSTTLAAMIDYLNNHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGNLGMQTLDMCL
KELVAKGLISREHAREKARSPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=708912 NK667_RS29490 WP_054616926.1 6675921..6676955(+) (pilT) [Pseudomonas nunensis strain In5]
ATGGATATCACTGAGCTGCTGGCATTCAGCGCCAAACAGGGTGCGTCCGACTTGCACCTGTCTGCCGGCCTGCCACCGAT
GATTCGCGTCGATGGCGATGTGCGGCGCATCAACCTGCCGGCCCTGGACCATAAACAGGTGCACGAGCTGATCTACGACA
TCATGAACGACACCCAGCGGGTGGACTTCGAGAAACACCTCGAAACTGACTTCTCCTTCGAAGTGCCGGGCGTGGCGCGC
TTCCGGGTCAACGCGTTCAACCAGAATCGCGGCGCCGGCGCGGTGTTCCGGACCATTCCGTCGAAGGTCCTGAGCATGGA
CGACCTCGGCATGGGCGAAGTGTTTCGCAAGATTACCGAAGCCCCCCGTGGCCTGGTGCTGGTGACGGGTCCGACCGGTT
CCGGCAAGTCCACCACCCTGGCAGCGATGATCGATTACCTGAACAACCATCGCCATCACCACATCCTCACCATCGAAGAT
CCAATCGAGTTCGTCCACGAATCACGCAAATGCCTGATCAATCAGCGCGAAGTCCATCGCGATACCCGCAGTTTCGCTAC
CGCGCTGCGCTCGGCGTTGCGCGAAGACCCGGATGTGATTCTGGTGGGCGAGATGCGCGATCTGGAAACCATTCGCCTGG
CATTGACCGCAGCCGAGACCGGCCACTTGGTGTTCGGCACGCTGCACACCACGTCGGCGGCGAAGACGATCGACCGGGTG
GTGGACGTGTTTCCGGGGGACGAGAAGTCGATGGTGCGCTCGATGCTGTCCGAGTCGTTGCTGGCGGTGGTGTCGCAGAC
GCTGGTGAAGAAGATCGGCGGCGGACGGATTGCGGCGCACGAAATCATGCTCGGTACGTCAGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGTCGATTCAGACCGGGGGCAACCTGGGGATGCAGACACTGGATATGTGTCTG
AAGGAACTGGTGGCCAAGGGTTTGATCAGCCGCGAGCATGCGCGGGAGAAGGCGCGGTCACCGGATAATTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Pseudomonas aeruginosa PAK

87.209

100

0.872
  pilT Acinetobacter baumannii D1279779

79.36

100

0.794
  pilT Acinetobacter baumannii strain A118

79.36

100

0.794
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

71.802

100

0.718
  pilT Legionella pneumophila strain ERS1305867

71.802

100

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Vibrio cholerae strain A1552

40

100

0.401