Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMC95_RS03755 Genome accession   NZ_CP101103
Coordinates   769474..770454 (+) Length   326 a.a.
NCBI ID   WP_370606902.1    Uniprot ID   -
Organism   Citrobacter werkmanii strain OT56     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 764474..775454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMC95_RS03725 (NMC95_03710) ansB 764987..766033 (+) 1047 WP_038633619.1 L-asparaginase 2 -
  NMC95_RS03730 (NMC95_03715) hemW 766150..767286 (-) 1137 WP_042310949.1 radical SAM family heme chaperone HemW -
  NMC95_RS03735 (NMC95_03720) - 767279..767872 (-) 594 WP_038633614.1 XTP/dITP diphosphatase -
  NMC95_RS03740 (NMC95_03725) yggU 767880..768170 (-) 291 WP_042310947.1 DUF167 family protein YggU -
  NMC95_RS03745 (NMC95_03730) - 768167..768733 (-) 567 WP_038633609.1 YggT family protein -
  NMC95_RS03750 (NMC95_03735) - 768752..769456 (-) 705 WP_370606901.1 YggS family pyridoxal phosphate-dependent enzyme -
  NMC95_RS03755 (NMC95_03740) pilT 769474..770454 (+) 981 WP_370606902.1 type IV pilus twitching motility protein PilT Machinery gene
  NMC95_RS03760 (NMC95_03745) ruvX 770451..770867 (-) 417 WP_038633601.1 Holliday junction resolvase RuvX -
  NMC95_RS03765 (NMC95_03750) - 770867..771430 (-) 564 WP_032940453.1 YqgE/AlgH family protein -
  NMC95_RS03770 (NMC95_03755) gshB 771542..772489 (-) 948 WP_042310939.1 glutathione synthase -
  NMC95_RS03775 (NMC95_03760) rsmE 772502..773233 (-) 732 WP_042310938.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NMC95_RS03780 (NMC95_03765) endA 773308..774015 (-) 708 WP_042310937.1 deoxyribonuclease I -
  NMC95_RS03785 (NMC95_03770) - 774110..774607 (-) 498 WP_042310936.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35972.14 Da        Isoelectric Point: 6.8706

>NTDB_id=708659 NMC95_RS03755 WP_370606902.1 769474..770454(+) (pilT) [Citrobacter werkmanii strain OT56]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIHGNVEIAPFTTPDVETLLMCWLSEQQQVQWQKQGQLDFAITLTNSRRLRA
SAFVHQQGISLALRLLPLDCPCLDYLQTPAALAELLHSENGLTLVTGATGSGKSTTLAAMVSYLNQHVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCTSFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEQDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGIMQTGQQTGMQTFTQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=708659 NMC95_RS03755 WP_370606902.1 769474..770454(+) (pilT) [Citrobacter werkmanii strain OT56]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCATACACGGAAATGTCGAAATCGCGCCGTTTACCACGCCTGACGTAGAGACGTTGCTGATGTGCTGGCTCAGCG
AGCAACAACAGGTACAGTGGCAGAAACAGGGGCAGCTGGATTTTGCCATTACGCTGACGAACTCACGGCGTCTGCGCGCG
AGCGCATTTGTTCATCAGCAGGGAATCTCTCTGGCGCTAAGGCTGTTGCCGCTTGATTGCCCATGTTTAGATTATCTCCA
GACTCCCGCCGCCTTAGCTGAGCTGCTGCATAGCGAAAATGGATTAACTCTGGTGACAGGCGCCACCGGCAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTGTCATATCTTAATCAGCACGTCGCGGGACATATTTTGACGCTGGAAGATCCCATTGAA
TATCGTTACACCAGCCAGCGTTGTCTCATTCAACAACGGGAGGTGGGCGTACACTGCACTTCTTTCGCCGCCGGTTTGCG
CGGCGCGCTGCGCGAAGATCCTGACGTCATTCTGCTGGGCGAGCTGCGCGATGTGGAAACCATTCGTCTGGCGTTAACGG
CTGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACACACGCGAGGGGCGGCGCAGGCCATTGCACGGCTGGTGGATACC
TTTCCCGCACAGGAGAAAGATCCGGTGCGTAATCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAACA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAACTGCTCGTCAACACGCCCGCCGTCGGTAACCTGATCCGTGAAGGGA
AAACGCATCAGCTACCCGGCATAATGCAAACCGGGCAACAGACAGGCATGCAGACGTTTACGCAGAGCCAGCAGCAGCGC
CAGGCGCAGGGACGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Acinetobacter baylyi ADP1

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.648

100

0.448
  pilT Legionella pneumophila strain ERS1305867

45.597

97.546

0.445
  pilT Legionella pneumophila strain Lp02

45.597

97.546

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.325

97.239

0.402
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

40

95.092

0.38
  pilU Acinetobacter baylyi ADP1

37.654

99.387

0.374