Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMC97_RS04550 Genome accession   NZ_CP101095
Coordinates   907574..908554 (+) Length   326 a.a.
NCBI ID   WP_003825426.1    Uniprot ID   A0AAJ1JNK1
Organism   Citrobacter portucalensis strain OT69     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 902574..913554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMC97_RS04510 (NMC97_04500) hemW 902801..903937 (-) 1137 WP_048236736.1 radical SAM family heme chaperone HemW -
  NMC97_RS04515 (NMC97_04505) - 903930..904523 (-) 594 WP_003825414.1 XTP/dITP diphosphatase -
  NMC97_RS04520 (NMC97_04510) yggU 904531..904821 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  NMC97_RS04525 (NMC97_04515) - 904818..905384 (-) 567 WP_003825417.1 YggT family protein -
  NMC97_RS04530 (NMC97_04520) - 905403..906107 (-) 705 WP_003825418.1 YggS family pyridoxal phosphate-dependent enzyme -
  NMC97_RS04535 (NMC97_04525) - 906125..906250 (+) 126 Protein_892 type IV pili twitching motility protein PilT -
  NMC97_RS04540 (NMC97_04535) - 906961..907380 (+) 420 WP_126956593.1 DUF3828 domain-containing protein -
  NMC97_RS04545 (NMC97_04540) - 907386..907556 (-) 171 Protein_894 YggS family pyridoxal phosphate enzyme -
  NMC97_RS04550 (NMC97_04545) pilT 907574..908554 (+) 981 WP_003825426.1 type IV pilus twitching motility protein PilT Machinery gene
  NMC97_RS04555 (NMC97_04550) ruvX 908551..908967 (-) 417 WP_003825428.1 Holliday junction resolvase RuvX -
  NMC97_RS04560 (NMC97_04555) - 908967..909530 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  NMC97_RS04565 (NMC97_04560) gshB 909707..910654 (-) 948 WP_003027083.1 glutathione synthase -
  NMC97_RS04570 (NMC97_04565) rsmE 910674..911405 (-) 732 WP_008785768.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NMC97_RS04575 (NMC97_04570) endA 911480..912187 (-) 708 WP_003825437.1 deoxyribonuclease I -
  NMC97_RS04580 (NMC97_04575) - 912282..912779 (-) 498 WP_126956595.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35912.06 Da        Isoelectric Point: 6.4963

>NTDB_id=708612 NMC97_RS04550 WP_003825426.1 907574..908554(+) (pilT) [Citrobacter portucalensis strain OT69]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVEIAPFTTPDVENLLMCWLNEQQQVQWQEQGQLDFAIALAGSQRLRA
SAFVHQQGTSLALRLLPLDCPRLDDLQTPDVIPELLRSENGLILVTGATGSGKSTTLAAMVAYLNQHVAGHILTLEDPIE
YRYTSQRCLIHQREVGVHCASFAAGLRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKLGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFTQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=708612 NMC97_RS04550 WP_003825426.1 907574..908554(+) (pilT) [Citrobacter portucalensis strain OT69]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGTGGAAAAGTCGAAATCGCACCCTTTACTACGCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAATG
AGCAACAACAGGTACAGTGGCAGGAACAGGGGCAGCTGGATTTTGCTATTGCGCTGGCAGGTTCCCAGCGGCTGCGCGCC
AGTGCTTTTGTTCATCAACAGGGAACCTCGCTGGCGCTAAGATTGCTCCCGCTTGATTGTCCACGTTTAGACGATCTCCA
GACCCCTGACGTCATACCTGAACTGCTGCGCAGTGAAAATGGGTTAATTCTGGTGACAGGCGCTACCGGTAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTGGCATATCTTAACCAGCACGTTGCGGGACATATTTTGACGCTGGAAGATCCCATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCACCAACGGGAGGTGGGCGTACACTGCGCTTCTTTTGCCGCCGGTTTACG
CGGCGCGCTGCGTGAAGATCCCGACGTCATTTTGCTGGGCGAACTGCGCGACGCGGAAACTATTCGCCTGGCGTTAACGG
CAGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGCTGGTGGATACC
TTTCCCGCACAGGAGAAAGATCCGGTGCGTAATCAGCTGGCAGACAGCCTACGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAGTTGGGAGGACGCGTGGCGCTATTTGAGCTGCTCGTCAACACGCCAGCCGTGGGCAATTTGATTCGTGAAGGGA
AAACGCATCAGCTACCCGGCGTAATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTACGCAGAGCCAGCAGCAGCGC
CAGGCGCAGGGTCGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

47.17

97.546

0.46
  pilT Legionella pneumophila strain ERS1305867

47.17

97.546

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.271

97.239

0.411
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38
  pilU Acinetobacter baylyi ADP1

37.152

99.08

0.368