Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMC99_RS04075 Genome accession   NZ_CP101092
Coordinates   834558..835538 (+) Length   326 a.a.
NCBI ID   WP_216421553.1    Uniprot ID   -
Organism   Citrobacter cronae strain OT70     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 829558..840538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMC99_RS04045 (NMC99_04045) ansB 830071..831117 (+) 1047 WP_038633619.1 L-asparaginase 2 -
  NMC99_RS04050 (NMC99_04050) hemW 831234..832370 (-) 1137 WP_216421554.1 radical SAM family heme chaperone HemW -
  NMC99_RS04055 (NMC99_04055) - 832363..832956 (-) 594 WP_038633614.1 XTP/dITP diphosphatase -
  NMC99_RS04060 (NMC99_04060) yggU 832964..833254 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  NMC99_RS04065 (NMC99_04065) - 833251..833817 (-) 567 WP_121529528.1 YggT family protein -
  NMC99_RS04070 (NMC99_04070) - 833836..834540 (-) 705 WP_199979281.1 YggS family pyridoxal phosphate-dependent enzyme -
  NMC99_RS04075 (NMC99_04075) pilT 834558..835538 (+) 981 WP_216421553.1 type IV pilus twitching motility protein PilT Machinery gene
  NMC99_RS04080 (NMC99_04080) ruvX 835535..835951 (-) 417 WP_038633601.1 Holliday junction resolvase RuvX -
  NMC99_RS04085 (NMC99_04085) - 835951..836514 (-) 564 WP_032940453.1 YqgE/AlgH family protein -
  NMC99_RS04090 (NMC99_04090) gshB 836626..837573 (-) 948 WP_216421552.1 glutathione synthase -
  NMC99_RS04095 (NMC99_04095) rsmE 837586..838317 (-) 732 WP_038633593.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NMC99_RS04100 (NMC99_04100) endA 838392..839099 (-) 708 WP_137347447.1 deoxyribonuclease I -
  NMC99_RS04105 (NMC99_04105) - 839194..839691 (-) 498 WP_137351598.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35890.20 Da        Isoelectric Point: 6.8182

>NTDB_id=708590 NMC99_RS04075 WP_216421553.1 834558..835538(+) (pilT) [Citrobacter cronae strain OT70]
MNMEEIVALSVKHYVSDLHLCNAWPARWRIRGNVEIAPFTTPDVETLLMCWLSEQQQVQWQKQGQLDFAITLANSRRLRA
SAFVHQQGISLALRLLPLDCPCLDDLQTPAALPELLHSENGLILVTGATGSGKSTTLAAMVAYLNQHVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAPEKDPVRNQLADSLRAVLSQKLEQDKQGGRVALFELLINTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFTQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=708590 NMC99_RS04075 WP_216421553.1 834558..835538(+) (pilT) [Citrobacter cronae strain OT70]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATTACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGCGGAAATGTCGAAATCGCGCCGTTTACCACGCCTGACGTAGAGACGTTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCAGAAACAGGGGCAGCTGGATTTTGCCATTACGCTGGCGAACTCACGGCGTCTGCGCGCG
AGCGCATTTGTGCATCAGCAGGGAATCTCTCTGGCGCTAAGGCTATTGCCGCTTGATTGCCCATGTTTGGATGATCTCCA
GACTCCCGCCGCCTTACCTGAGTTGCTGCACAGTGAAAATGGATTAATTCTGGTGACCGGCGCTACCGGCAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTGGCATATCTTAATCAGCACGTCGCGGGACATATTTTGACGCTGGAAGATCCCATTGAA
TATCGTTACACCAGCCAGCGTTGTCTCATTCAACAACGGGAGGTGGGCGTACACTGCGCTTCTTTCGCCGCCGGTTTGCG
CGGCGCGCTACGCGAAGATCCTGATGTCATTCTGCTGGGCGAGCTGCGCGACGTAGAAACCATTCGTCTGGCGTTAACGG
CTGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACACACGCGAGGGGCGGCTCAGGCGATTGCGCGGCTGGTGGATACT
TTTCCCGCACCGGAGAAAGATCCGGTGCGTAATCAACTGGCAGACAGCCTGCGGGCAGTGCTTTCGCAAAAGCTGGAACA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAACTGCTCATCAACACGCCCGCCGTCGGTAACCTGATCCGTGAAGGGA
AAACGCATCAGCTACCCGGCGTAATTCAAACCGGGCAACAGACAGGCATGCAGACGTTTACGCAGAGCCTGCAGCAGCGC
CAGGCGCAGGGACGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

46.273

98.773

0.457
  pilT Legionella pneumophila strain Lp02

46.273

98.773

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.121

98.466

0.405
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Acinetobacter baylyi ADP1

38.462

99.693

0.383
  pilU Vibrio cholerae strain A1552

40

95.092

0.38