Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMD06_RS03950 Genome accession   NZ_CP101082
Coordinates   820184..821164 (+) Length   326 a.a.
NCBI ID   WP_370604828.1    Uniprot ID   -
Organism   Citrobacter braakii strain OT129     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 815184..826164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD06_RS03920 (NMD06_03915) - 815560..816546 (-) 987 WP_370604826.1 TRAP transporter substrate-binding protein -
  NMD06_RS03925 (NMD06_03920) hemW 816860..817996 (-) 1137 WP_114681113.1 radical SAM family heme chaperone HemW -
  NMD06_RS03930 (NMD06_03925) - 817989..818582 (-) 594 WP_016154402.1 XTP/dITP diphosphatase -
  NMD06_RS03935 (NMD06_03930) yggU 818590..818880 (-) 291 WP_005123286.1 DUF167 family protein YggU -
  NMD06_RS03940 (NMD06_03935) - 818877..819443 (-) 567 WP_016154401.1 YggT family protein -
  NMD06_RS03945 (NMD06_03940) - 819462..820166 (-) 705 WP_199146396.1 YggS family pyridoxal phosphate-dependent enzyme -
  NMD06_RS03950 (NMD06_03945) pilT 820184..821164 (+) 981 WP_370604828.1 type IV pilus twitching motility protein PilT Machinery gene
  NMD06_RS03955 (NMD06_03950) ruvX 821161..821577 (-) 417 WP_200109562.1 Holliday junction resolvase RuvX -
  NMD06_RS03960 (NMD06_03955) - 821577..822140 (-) 564 WP_016154395.1 YqgE/AlgH family protein -
  NMD06_RS03965 (NMD06_03960) gshB 822317..823264 (-) 948 WP_370604830.1 glutathione synthase -
  NMD06_RS03970 (NMD06_03965) rsmE 823277..824008 (-) 732 WP_267588312.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NMD06_RS03975 (NMD06_03970) endA 824084..824791 (-) 708 WP_267588313.1 deoxyribonuclease I -
  NMD06_RS03980 (NMD06_03975) - 824886..825383 (-) 498 WP_016157438.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36107.21 Da        Isoelectric Point: 6.3375

>NTDB_id=708546 NMD06_RS03950 WP_370604828.1 820184..821164(+) (pilT) [Citrobacter braakii strain OT129]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVETAPFTTPDVENLLMCWLSEQQQVQWREQGQIDFALTLANSWRLRA
SAFAHQQGTSLALRLLPLECPRLDDLQTPEAIPELLHCENGLVLVTGATGSGKSTTLAAMVEYLNQHIAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAITRLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=708546 NMD06_RS03950 WP_370604828.1 820184..821164(+) (pilT) [Citrobacter braakii strain OT129]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGTGGAAAAGTCGAAACCGCACCATTTACTACGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGATTGATTTTGCCCTTACGTTAGCGAACTCCTGGCGTCTGCGCGCC
AGCGCATTTGCTCATCAGCAGGGAACCTCGCTGGCGCTAAGATTGCTACCGCTTGAATGTCCTCGTTTAGACGATCTCCA
GACTCCCGAGGCCATACCTGAACTGCTGCACTGTGAAAATGGATTAGTTCTGGTTACTGGCGCCACCGGAAGCGGTAAAT
CAACCACTCTGGCGGCGATGGTGGAGTACCTTAATCAGCATATTGCCGGGCATATTCTGACGCTGGAAGATCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATCCAACAGCGGGAGGTGGGCGTACACTGCGCTTCTTTTGCTGCCGGTTTGCG
CGGCGCGTTACGCGAAGATCCCGACGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACTATTCGCCTGGCCTTAACGG
CAGCGGAGACCGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGGGCGGCGCAAGCTATCACGCGACTGGTGGACACC
TTTCCCGCTCAGGAGAAAGATCCGGTGCGTAACCAACTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGTTGGAGGA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAGCTGCTAGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGTGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCAGCAGCAGCGC
CAGGCACAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(56-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.07

100

0.512
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.07

100

0.512
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Legionella pneumophila strain ERS1305867

47.799

97.546

0.466
  pilT Legionella pneumophila strain Lp02

47.799

97.546

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.64

97.239

0.405
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362