Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMD11_RS04335 Genome accession   NZ_CP101066
Coordinates   882101..883081 (+) Length   326 a.a.
NCBI ID   WP_193157133.1    Uniprot ID   -
Organism   Citrobacter cronae strain OT154     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 877101..888081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD11_RS04305 (NMD11_04310) ansB 877614..878660 (+) 1047 WP_038633619.1 L-asparaginase 2 -
  NMD11_RS04310 (NMD11_04315) hemW 878777..879913 (-) 1137 WP_160955389.1 radical SAM family heme chaperone HemW -
  NMD11_RS04315 (NMD11_04320) - 879906..880499 (-) 594 WP_038633614.1 XTP/dITP diphosphatase -
  NMD11_RS04320 (NMD11_04325) yggU 880507..880797 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  NMD11_RS04325 (NMD11_04330) - 880794..881360 (-) 567 WP_160955390.1 YggT family protein -
  NMD11_RS04330 (NMD11_04335) - 881379..882083 (-) 705 WP_038633606.1 YggS family pyridoxal phosphate-dependent enzyme -
  NMD11_RS04335 (NMD11_04340) pilT 882101..883081 (+) 981 WP_193157133.1 type IV pilus twitching motility protein PilT Machinery gene
  NMD11_RS04340 (NMD11_04345) ruvX 883078..883494 (-) 417 WP_038633601.1 Holliday junction resolvase RuvX -
  NMD11_RS04345 (NMD11_04350) - 883494..884057 (-) 564 WP_032940453.1 YqgE/AlgH family protein -
  NMD11_RS04350 (NMD11_04355) gshB 884169..885116 (-) 948 WP_038633598.1 glutathione synthase -
  NMD11_RS04355 (NMD11_04360) rsmE 885129..885860 (-) 732 WP_038633593.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NMD11_RS04360 (NMD11_04365) endA 885935..886642 (-) 708 WP_085048753.1 deoxyribonuclease I -
  NMD11_RS04365 (NMD11_04370) - 886737..887234 (-) 498 WP_038633587.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35806.11 Da        Isoelectric Point: 6.6497

>NTDB_id=708455 NMD11_RS04335 WP_193157133.1 882101..883081(+) (pilT) [Citrobacter cronae strain OT154]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGNVEIAPFTTPDVETLLMCWLSEQQQVQWQKQGQLDFAITLANSRRLRA
SAFVHQQGISLALRLLPLDCPCLDDLQTPAALPELLHSENGLILVTGATGSGKSTTLAAMVAYLNQHVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAPEKDPVRNQLADSLRAVLSQKLEQDKQGGCVALFELLINTPAVGNLIREGKTHQLPGVMQTGQQTGMQTFTQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=708455 NMD11_RS04335 WP_193157133.1 882101..883081(+) (pilT) [Citrobacter cronae strain OT154]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGCGGAAATGTCGAAATCGCGCCGTTTACCACGCCTGACGTAGAGACGTTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCAGAAACAGGGGCAGCTGGATTTTGCCATTACGCTGGCGAACTCACGGCGTCTACGCGCG
AGCGCATTTGTGCATCAGCAGGGAATCTCTCTGGCGCTAAGGCTATTGCCGCTTGATTGCCCATGTTTGGATGATCTCCA
GACTCCCGCCGCCTTACCTGAGTTGCTGCACAGTGAAAATGGATTAATTCTGGTGACCGGCGCTACCGGCAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTGGCATATCTTAATCAGCACGTCGCGGGACATATTTTGACGCTGGAAGATCCCATTGAA
TATCGTTACACCAGCCAGCGTTGTCTCATTCAACAACGGGAGGTGGGCGTACACTGCGCTTCTTTCGCCGCCGGTTTGCG
CGGCGCGCTGCGCGAAGATCCTGATGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGTCTGGCGTTAACGG
CTGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACACACGCGAGGGGCGGCTCAGGCGATTGCGCGGCTGGTGGATACC
TTTCCTGCACCGGAGAAAGATCCGGTGCGTAATCAGCTGGCAGACAGCCTGCGGGCAGTGCTTTCGCAAAAGCTGGAACA
AGATAAGCAGGGAGGATGCGTGGCGCTATTCGAACTGCTCATCAACACGCCCGCCGTCGGTAACCTGATCCGTGAAGGGA
AAACGCATCAGCTACCCGGCGTAATGCAAACCGGGCAACAGACAGGCATGCAGACGTTTACGCAAAGCCTGCAGCAGCGC
CAGGCGCAGGGACGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(66-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Pseudomonas stutzeri DSM 10701

45.566

100

0.457
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Legionella pneumophila strain ERS1305867

45.652

98.773

0.451
  pilT Legionella pneumophila strain Lp02

45.652

98.773

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.304

98.773

0.408
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Acinetobacter baylyi ADP1

38.462

99.693

0.383
  pilU Vibrio cholerae strain A1552

40

95.092

0.38