Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMD12_RS03750 Genome accession   NZ_CP101062
Coordinates   771778..772758 (+) Length   326 a.a.
NCBI ID   WP_370609366.1    Uniprot ID   -
Organism   Citrobacter portucalensis strain OT163     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 766778..777758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD12_RS03720 (NMD12_03720) ansB 767293..768339 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  NMD12_RS03725 (NMD12_03725) hemW 768454..769590 (-) 1137 WP_003825412.1 radical SAM family heme chaperone HemW -
  NMD12_RS03730 (NMD12_03730) - 769583..770176 (-) 594 WP_057067563.1 XTP/dITP diphosphatase -
  NMD12_RS03735 (NMD12_03735) yggU 770184..770474 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  NMD12_RS03740 (NMD12_03740) - 770471..771037 (-) 567 WP_003825417.1 YggT family protein -
  NMD12_RS03745 (NMD12_03745) - 771056..771760 (-) 705 WP_057067562.1 YggS family pyridoxal phosphate-dependent enzyme -
  NMD12_RS03750 (NMD12_03750) pilT 771778..772758 (+) 981 WP_370609366.1 type IV pilus twitching motility protein PilT Machinery gene
  NMD12_RS03755 (NMD12_03755) ruvX 772755..773171 (-) 417 WP_008785771.1 Holliday junction resolvase RuvX -
  NMD12_RS03760 (NMD12_03760) - 773171..773734 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  NMD12_RS03765 (NMD12_03765) gshB 773911..774858 (-) 948 WP_003027083.1 glutathione synthase -
  NMD12_RS03770 (NMD12_03770) rsmE 774878..775609 (-) 732 WP_186413007.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NMD12_RS03775 (NMD12_03775) endA 775684..776391 (-) 708 WP_008785767.1 deoxyribonuclease I -
  NMD12_RS03780 (NMD12_03780) - 776486..776983 (-) 498 WP_003825440.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35898.04 Da        Isoelectric Point: 6.2312

>NTDB_id=708432 NMD12_RS03750 WP_370609366.1 771778..772758(+) (pilT) [Citrobacter portucalensis strain OT163]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVEIAPFTTPDVENLLMCWLSEQQQVQWREQGQVDFAIALAGSQRLRA
SAFVHQQGTSLALRLLPLDCPRLDDLQTPDVIPELLRSENGLILVTGATGSGKSMTLAAMVEYLNQHVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FSATEKEPVRNQLADSLRAVLSQTLEEDKLGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRI

Nucleotide


Download         Length: 981 bp        

>NTDB_id=708432 NMD12_RS03750 WP_370609366.1 771778..772758(+) (pilT) [Citrobacter portucalensis strain OT163]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGTGGAAAAGTCGAAATCGCACCCTTTACTACGCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGGTAGATTTTGCTATTGCGCTGGCAGGTTCCCAGCGGCTGCGCGCC
AGTGCTTTTGTTCATCAACAGGGAACCTCGCTGGCGCTAAGATTGCTCCCGCTTGATTGTCCACGTTTAGACGATCTCCA
GACCCCTGACGTCATACCTGAACTGCTGCGCAGTGAAAATGGGTTAATTCTGGTGACAGGCGCTACCGGTAGCGGTAAAT
CAATGACCCTGGCGGCGATGGTAGAGTACCTTAATCAGCATGTTGCGGGGCATATTCTGACGCTGGAAGACCCGATTGAA
TATCGTTACACCAGCCAGCGTTGTCTTATTCAACAACGGGAGGTGGGCGTACACTGCGCTTCTTTTGCCGCTGGTTTACG
CGGCGCGCTGCGTGAAGATCCCGACGTCATTTTGCTGGGCGAACTGCGCGACGCGGAAACCATTCGTCTGGCATTAACGG
CAGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGGGCGGCACAGGCTATCGCGCGGCTGGTGGACACC
TTTTCGGCAACAGAGAAAGAGCCGGTGCGTAACCAGTTAGCAGACAGCCTGCGGGCGGTACTTTCGCAAACGCTGGAGGA
AGATAAGCTGGGAGGACGCGTGGCGCTATTTGAGCTGCTCGTCAACACGCCAGCCGTGGGCAATTTGATTCGTGAAGGGA
AAACGCATCAGCTGCCCGGCGTAATTCAAACCGGGCAACAGACAGGTATGCAGACATTTGCACAAAGTCAGCAGCAGCGT
CAGGCGCAGGGACGGATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

46.364

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.061

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

45.593

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.289

100

0.457
  pilT Acinetobacter baylyi ADP1

45.289

100

0.457
  pilT Acinetobacter baumannii strain A118

44.985

100

0.454
  pilT Acinetobacter baumannii D1279779

44.985

100

0.454
  pilT Acinetobacter nosocomialis M2

44.681

100

0.451
  pilT Legionella pneumophila strain ERS1305867

46.226

97.546

0.451
  pilT Legionella pneumophila strain Lp02

46.226

97.546

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.325

97.239

0.402
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365