Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMD21_RS04045 Genome accession   NZ_CP101060
Coordinates   825034..826014 (+) Length   326 a.a.
NCBI ID   WP_370615287.1    Uniprot ID   -
Organism   Citrobacter portucalensis strain OT205     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 820034..831014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD21_RS04015 (NMD21_04015) ansB 820547..821593 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  NMD21_RS04020 (NMD21_04020) hemW 821710..822846 (-) 1137 WP_123267928.1 radical SAM family heme chaperone HemW -
  NMD21_RS04025 (NMD21_04025) - 822839..823432 (-) 594 WP_079938668.1 XTP/dITP diphosphatase -
  NMD21_RS04030 (NMD21_04030) yggU 823440..823730 (-) 291 WP_049015279.1 DUF167 family protein YggU -
  NMD21_RS04035 (NMD21_04035) - 823727..824293 (-) 567 WP_003825417.1 YggT family protein -
  NMD21_RS04040 (NMD21_04040) - 824312..825016 (-) 705 WP_286636733.1 YggS family pyridoxal phosphate-dependent enzyme -
  NMD21_RS04045 (NMD21_04045) pilT 825034..826014 (+) 981 WP_370615287.1 type IV pilus twitching motility protein PilT Machinery gene
  NMD21_RS04050 (NMD21_04050) ruvX 826011..826427 (-) 417 WP_008785771.1 Holliday junction resolvase RuvX -
  NMD21_RS04055 (NMD21_04055) - 826427..826990 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  NMD21_RS04060 (NMD21_04060) gshB 827167..828114 (-) 948 WP_003027083.1 glutathione synthase -
  NMD21_RS04065 (NMD21_04065) rsmE 828134..828865 (-) 732 WP_370615289.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NMD21_RS04070 (NMD21_04070) endA 828940..829647 (-) 708 WP_370615291.1 deoxyribonuclease I -
  NMD21_RS04075 (NMD21_04075) - 829742..830239 (-) 498 WP_003825440.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36005.14 Da        Isoelectric Point: 6.5833

>NTDB_id=708409 NMD21_RS04045 WP_370615287.1 825034..826014(+) (pilT) [Citrobacter portucalensis strain OT205]
MNMEEIVVLSVKHNVSDLHLCNAWPARWRIRGKVDIAPFTTPDVENLLMCWLSEQQQVQWREQGQVDFAIALAGSRRLRA
SAFAHQHGTSLALRLLPLDCPRLDDLQTPDVIPELLRSENGLILVTGATGSGKSTTLAAMVEYLNQQVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRSALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRI

Nucleotide


Download         Length: 981 bp        

>NTDB_id=708409 NMD21_RS04045 WP_370615287.1 825034..826014(+) (pilT) [Citrobacter portucalensis strain OT205]
ATGAATATGGAAGAAATAGTGGTCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGTGGAAAAGTCGACATCGCACCATTTACTACGCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGGTAGATTTTGCTATTGCGCTTGCAGGTTCCCGGCGGCTGCGCGCC
AGTGCTTTTGCTCATCAACATGGAACCTCGCTGGCGCTAAGATTGCTCCCGCTTGATTGCCCACGTTTAGACGATCTCCA
GACCCCTGACGTCATACCTGAACTGCTGCGCAGTGAAAATGGGTTAATTCTGGTGACAGGCGCTACCGGTAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTTGAGTACCTTAATCAGCAAGTTGCGGGGCACATTCTGACGCTGGAAGACCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAACGGGAGGTGGGCGTGCACTGCGCTTCTTTTGCCGCTGGTTTACG
CAGCGCGTTACGTGAAGATCCCGACGTCATTTTGCTGGGCGAGCTGCGCGATGTGGAAACCATTCGTCTGGCGTTAACCG
CAGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGGGCGGCGCAGGCTATCGCACGGCTGGTGGATACC
TTTCCCGCACAGGAAAAAGATCCGGTGCGTAACCAACTGGCAGACAGCCTGCGGGCAGTGCTTTCGCAAAAGCTGGAGGA
AGATAAACAGGGAGGGCGCGTGGCGCTATTTGAGCTGCTCGTCAACACGCCAGCCGTGGGCAATTTGATTCGTGAAGGGA
AAACGCATCAGCTACCCGGCGTAATTCAAACCGGGCAGCAGACAGGTATGCAGACATTTGCACAAAGCCAGCAGCAGCGT
CAGGCGCAGGGGCGGATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(60-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Neisseria meningitidis 8013

46.667

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.364

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

45.897

100

0.463
  pilT Acinetobacter baylyi ADP1

45.593

100

0.46
  pilT Acinetobacter baumannii D1279779

45.593

100

0.46
  pilT Acinetobacter baumannii strain A118

45.593

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.593

100

0.46
  pilT Acinetobacter nosocomialis M2

45.289

100

0.457
  pilT Legionella pneumophila strain ERS1305867

46.855

97.546

0.457
  pilT Legionella pneumophila strain Lp02

46.855

97.546

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.956

97.239

0.408
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

39.355

95.092

0.374
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365