Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMD08_RS04740 Genome accession   NZ_CP101036
Coordinates   979696..980676 (+) Length   326 a.a.
NCBI ID   WP_151218354.1    Uniprot ID   -
Organism   Citrobacter meridianamericanus strain OT146     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 974696..985676
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD08_RS04710 (NMD08_04680) - 975073..976059 (-) 987 WP_151218358.1 TRAP transporter substrate-binding protein -
  NMD08_RS04715 (NMD08_04685) hemW 976372..977508 (-) 1137 WP_151224320.1 radical SAM family heme chaperone HemW -
  NMD08_RS04720 (NMD08_04690) - 977501..978094 (-) 594 WP_370619656.1 XTP/dITP diphosphatase -
  NMD08_RS04725 (NMD08_04695) yggU 978102..978392 (-) 291 WP_005123286.1 DUF167 family protein YggU -
  NMD08_RS04730 (NMD08_04700) - 978389..978955 (-) 567 WP_003027097.1 YggT family protein -
  NMD08_RS04735 (NMD08_04705) - 978974..979678 (-) 705 WP_151218355.1 YggS family pyridoxal phosphate-dependent enzyme -
  NMD08_RS04740 (NMD08_04710) pilT 979696..980676 (+) 981 WP_151218354.1 type IV pilus twitching motility protein PilT Machinery gene
  NMD08_RS04745 (NMD08_04715) ruvX 980673..981089 (-) 417 WP_006686843.1 Holliday junction resolvase RuvX -
  NMD08_RS04750 (NMD08_04720) - 981089..981652 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  NMD08_RS04755 (NMD08_04725) gshB 981764..982711 (-) 948 WP_003027083.1 glutathione synthase -
  NMD08_RS04760 (NMD08_04730) rsmE 982724..983455 (-) 732 WP_187253186.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NMD08_RS04765 (NMD08_04735) endA 983530..984237 (-) 708 WP_151218353.1 deoxyribonuclease I -
  NMD08_RS04770 (NMD08_04740) - 984332..984829 (-) 498 WP_151218352.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35979.05 Da        Isoelectric Point: 6.5700

>NTDB_id=708299 NMD08_RS04740 WP_151218354.1 979696..980676(+) (pilT) [Citrobacter meridianamericanus strain OT146]
MNMEEIVALSVKHNVSDLHLCNAWPARWRRRGKVESAPFTAPDVENLLMCWLSEQQQVQLQEQGQIDFAVTLTDSRRLRV
SAFAHQQGTSLALRLLPLDCPHLDDLQTPTVIPELLHSENGLILVTGATGSGKSTTLAAMVEYLNQHSEGHILTLEDPIE
YRYTSRRCLIQQREVGVHCTSFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAVQAIARLVDS
FAATEKDPVRNQLADSLKAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPSVIQTGQQTGMQSFAQSKQQR
QALGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=708299 NMD08_RS04740 WP_151218354.1 979696..980676(+) (pilT) [Citrobacter meridianamericanus strain OT146]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCAGGCGTGGAAAAGTCGAAAGTGCGCCATTTACCGCGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTTGCAGGAGCAGGGGCAGATTGATTTTGCCGTTACTCTGACAGACTCCCGGCGGCTGCGCGTC
AGCGCATTTGCCCATCAACAGGGAACGTCGCTGGCGTTAAGACTGCTACCGCTGGATTGTCCTCATTTAGACGATCTCCA
GACCCCCACGGTCATACCTGAACTGCTGCACAGTGAAAATGGATTAATTCTGGTGACGGGCGCCACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGGAGTATCTTAATCAGCATAGTGAGGGACACATTCTGACGCTGGAAGATCCTATTGAA
TATCGCTACACCAGTCGACGTTGTCTGATTCAACAGCGGGAGGTGGGCGTACACTGCACCTCTTTTGCCGCCGGTTTGCG
CGGTGCGTTACGCGAAGATCCTGACGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTAACGG
CGGCGGAGACCGGACATCTGGTGCTGGCAACGTTACATACGCGCGGGGCGGTGCAGGCTATCGCACGGCTGGTGGATTCC
TTTGCAGCAACAGAGAAAGATCCGGTGCGTAACCAGCTGGCAGACAGCCTAAAGGCGGTTCTTTCGCAAAAGCTGGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAGCTGCTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACACATCAGCTACCCAGCGTGATTCAAACCGGGCAGCAGACAGGTATGCAGTCGTTTGCACAAAGCAAGCAGCAGCGG
CAGGCGCTGGGGCGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(54-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

46.809

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.505

100

0.469
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

47.152

96.933

0.457
  pilT Legionella pneumophila strain ERS1305867

46.541

97.546

0.454
  pilT Legionella pneumophila strain Lp02

46.541

97.546

0.454
  pilT Acinetobacter baumannii D1279779

46.395

97.853

0.454
  pilT Acinetobacter nosocomialis M2

46.395

97.853

0.454
  pilT Acinetobacter baumannii strain A118

46.395

97.853

0.454
  pilT Pseudomonas aeruginosa PAK

45.886

96.933

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.357

96.319

0.408
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Pseudomonas stutzeri DSM 10701

38.75

98.16

0.38
  pilU Acinetobacter baylyi ADP1

37.267

98.773

0.368