Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMD13_RS02000 Genome accession   NZ_CP101034
Coordinates   414065..415099 (+) Length   344 a.a.
NCBI ID   WP_151189058.1    Uniprot ID   A0A9X7N4C0
Organism   Pseudomonas nitroreducens strain OT171     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 409065..420099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD13_RS01975 (NMD13_01970) - 409462..411429 (-) 1968 WP_275609790.1 dynamin-like GTPase family protein -
  NMD13_RS01980 (NMD13_01975) - 411548..411838 (-) 291 WP_331315640.1 DUF167 domain-containing protein -
  NMD13_RS01985 (NMD13_01980) - 411844..412437 (-) 594 WP_275609792.1 YggT family protein -
  NMD13_RS01990 (NMD13_01985) proC 412451..413284 (-) 834 WP_275609793.1 pyrroline-5-carboxylate reductase -
  NMD13_RS01995 (NMD13_01990) - 413302..413991 (-) 690 WP_370600537.1 YggS family pyridoxal phosphate-dependent enzyme -
  NMD13_RS02000 (NMD13_01995) pilT 414065..415099 (+) 1035 WP_151189058.1 type IV pilus twitching motility protein PilT Machinery gene
  NMD13_RS02005 (NMD13_02000) pilU 415206..416351 (+) 1146 WP_370600538.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMD13_RS02010 (NMD13_02005) - 416444..416848 (+) 405 WP_275609796.1 TM2 domain-containing protein -
  NMD13_RS02015 (NMD13_02010) - 417074..418447 (+) 1374 WP_370600539.1 pyridoxal-phosphate dependent enzyme -
  NMD13_RS02020 (NMD13_02015) - 418444..419613 (+) 1170 WP_370600540.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37898.76 Da        Isoelectric Point: 6.7752

>NTDB_id=708265 NMD13_RS02000 WP_151189058.1 414065..415099(+) (pilT) [Pseudomonas nitroreducens strain OT171]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFKRVSDVPRGLVLVTGPTGSGKSTSLAAMLDYLNNTKYSHILTVED
PIEFVHESKKCLVNQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGAIGMQTLDMCL
KGLVAKGLVTREAAKEKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=708265 NMD13_RS02000 WP_151189058.1 414065..415099(+) (pilT) [Pseudomonas nitroreducens strain OT171]
ATGGATATTACCGAGTTGCTCGCCTTCAGCGCCAAGCAGGGCGCTTCGGACCTGCACCTCTCGGCCGGTCTGCCGCCGAT
GATCCGCGTCGATGGCGATGTTCGCCGGATCAACCTGCCGCCCCTGGAGCATAAGCAGGTTCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAAGAATTCCTGGAGACCGACTTCTCCTTCGAAGTCCCCGGGGTCGCCCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGCGCCGTGTTCCGTACCATTCCCTCCAAGGTACTCACGATGGA
GGACCTGGGCATGGGGGAAGTGTTCAAACGTGTTTCCGACGTGCCGCGCGGCCTGGTGCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCCACCTCGCTGGCGGCGATGCTCGACTACCTGAACAACACCAAGTACAGCCACATCCTCACCGTCGAAGAC
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTACACCGCGACACCCTCGGCTTCAACGA
GGCGCTGCGCTCGGCCCTGCGTGAGGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCCGCCGAGGAAAAGTCCATGGTCCGTTCCATGCTCTCCGAATCGCTGCAGGCGGTGATCTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGCCGTGTCGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGCGGTGCGATCGGCATGCAGACCCTGGACATGTGCTTG
AAGGGCCTGGTCGCCAAGGGTCTGGTGACCCGCGAGGCGGCCAAGGAGAAGGCCAAGATTCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

95.349

100

0.953
  pilT Pseudomonas stutzeri DSM 10701

92.733

100

0.927
  pilT Acinetobacter baumannii D1279779

84.593

100

0.846
  pilT Acinetobacter baumannii strain A118

84.593

100

0.846
  pilT Acinetobacter nosocomialis M2

84.302

100

0.843
  pilT Acinetobacter baylyi ADP1

82.267

100

0.823
  pilT Legionella pneumophila strain Lp02

76.331

98.256

0.75
  pilT Legionella pneumophila strain ERS1305867

76.331

98.256

0.75
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.941

98.837

0.672
  pilT Vibrio cholerae strain A1552

67.941

98.837

0.672
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39