Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMD28_RS04165 Genome accession   NZ_CP101001
Coordinates   854500..855480 (+) Length   326 a.a.
NCBI ID   WP_001324408.1    Uniprot ID   A0A0E1LFY8
Organism   Escherichia coli strain ET120     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 849500..860480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD28_RS04135 (NMD28_04125) yggM 850015..851022 (+) 1008 WP_000745217.1 DUF1202 family protein -
  NMD28_RS04140 (NMD28_04130) hemW 851177..852313 (-) 1137 WP_000239928.1 radical SAM family heme chaperone HemW -
  NMD28_RS04145 (NMD28_04135) rdgB 852306..852899 (-) 594 WP_001174743.1 XTP/dITP diphosphatase -
  NMD28_RS04150 (NMD28_04140) yggU 852907..853197 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  NMD28_RS04155 (NMD28_04145) yggT 853194..853760 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  NMD28_RS04160 (NMD28_04150) yggS 853778..854482 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  NMD28_RS04165 (NMD28_04155) pilT 854500..855480 (+) 981 WP_001324408.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMD28_RS04170 (NMD28_04160) ruvX 855643..856059 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  NMD28_RS04175 (NMD28_04165) yqgE 856059..856622 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  NMD28_RS04180 (NMD28_04170) gshB 856731..857681 (-) 951 WP_000593273.1 glutathione synthase -
  NMD28_RS04185 (NMD28_04175) rsmE 857694..858425 (-) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NMD28_RS04190 (NMD28_04180) endA 858505..859212 (-) 708 WP_000286500.1 deoxyribonuclease I -
  NMD28_RS04195 (NMD28_04185) yggI 859307..859804 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35873.07 Da        Isoelectric Point: 5.5857

>NTDB_id=708061 NMD28_RS04165 WP_001324408.1 854500..855480(+) (pilT) [Escherichia coli strain ET120]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGCVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=708061 NMD28_RS04165 WP_001324408.1 854500..855480(+) (pilT) [Escherichia coli strain ET120]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACTTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTAACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGATGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTTTCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E1LFY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362