Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMD30_RS03825 Genome accession   NZ_CP100994
Coordinates   778876..779856 (+) Length   326 a.a.
NCBI ID   WP_072020095.1    Uniprot ID   -
Organism   Escherichia coli strain ET201     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 773876..784856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD30_RS03795 (NMD30_03785) - 774259..775245 (-) 987 WP_000784005.1 TRAP transporter substrate-binding protein -
  NMD30_RS03800 (NMD30_03790) hemW 775553..776689 (-) 1137 WP_000239939.1 radical SAM family heme chaperone HemW -
  NMD30_RS03805 (NMD30_03795) rdgB 776682..777275 (-) 594 WP_001174751.1 XTP/dITP diphosphatase -
  NMD30_RS03810 (NMD30_03800) yggU 777283..777573 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  NMD30_RS03815 (NMD30_03805) yggT 777570..778136 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  NMD30_RS03820 (NMD30_03810) yggS 778154..778858 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  NMD30_RS03825 (NMD30_03815) pilT 778876..779856 (+) 981 WP_072020095.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMD30_RS03830 (NMD30_03820) ruvX 780032..780448 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  NMD30_RS03835 (NMD30_03825) yqgE 780448..781011 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  NMD30_RS03840 (NMD30_03830) gshB 781120..782070 (-) 951 WP_000593273.1 glutathione synthase -
  NMD30_RS03845 (NMD30_03835) rsmE 782083..782814 (-) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NMD30_RS03850 (NMD30_03840) endA 782894..783601 (-) 708 WP_000286489.1 deoxyribonuclease I -
  NMD30_RS03855 (NMD30_03845) yggI 783696..784193 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35899.09 Da        Isoelectric Point: 5.5860

>NTDB_id=708017 NMD30_RS03825 WP_072020095.1 778876..779856(+) (pilT) [Escherichia coli strain ET201]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=708017 NMD30_RS03825 WP_072020095.1 778876..779856(+) (pilT) [Escherichia coli strain ET201]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACTTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371