Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMD37_RS04250 Genome accession   NZ_CP100966
Coordinates   881674..882654 (+) Length   326 a.a.
NCBI ID   WP_001625905.1    Uniprot ID   A0A2T3U361
Organism   Escherichia coli strain ET350     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 876674..887654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD37_RS04220 (NMD37_04215) yggM 877101..878108 (+) 1008 WP_000745239.1 DUF1202 family protein -
  NMD37_RS04225 (NMD37_04220) hemW 878351..879487 (-) 1137 WP_001625906.1 radical SAM family heme chaperone HemW -
  NMD37_RS04230 (NMD37_04225) rdgB 879480..880073 (-) 594 WP_001174748.1 XTP/dITP diphosphatase -
  NMD37_RS04235 (NMD37_04230) yggU 880081..880371 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  NMD37_RS04240 (NMD37_04235) yggT 880368..880934 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  NMD37_RS04245 (NMD37_04240) yggS 880952..881656 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  NMD37_RS04250 (NMD37_04245) pilT 881674..882654 (+) 981 WP_001625905.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMD37_RS04255 (NMD37_04250) ruvX 882845..883261 (-) 417 WP_000017109.1 Holliday junction resolvase RuvX -
  NMD37_RS04260 (NMD37_04255) yqgE 883261..883824 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  NMD37_RS04265 (NMD37_04260) gshB 883933..884883 (-) 951 WP_000593273.1 glutathione synthase -
  NMD37_RS04270 (NMD37_04265) rsmE 884896..885627 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NMD37_RS04275 (NMD37_04270) endA 885707..886414 (-) 708 WP_000286500.1 deoxyribonuclease I -
  NMD37_RS04280 (NMD37_04275) yggI 886509..887006 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35929.12 Da        Isoelectric Point: 5.5860

>NTDB_id=707906 NMD37_RS04250 WP_001625905.1 881674..882654(+) (pilT) [Escherichia coli strain ET350]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLTENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=707906 NMD37_RS04250 WP_001625905.1 881674..882654(+) (pilT) [Escherichia coli strain ET350]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGACGGAAAACCAGCGATTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCTACGGGGAGTGGAAAAT
CTACCACGCTGGCGGCGATGGTTGGTTATCTCAATCAACATGCTGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTGCATTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTTGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3U361

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371