Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MRY16398_RS04400 Genome accession   NZ_AP018756
Coordinates   908694..909674 (+) Length   326 a.a.
NCBI ID   WP_125123889.1    Uniprot ID   A0A348D8J9
Organism   Phytobacter sp. MRY16-398     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 903694..914674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRY16398_RS04370 (MRY16398_08660) - 904090..905076 (-) 987 WP_079497170.1 TRAP transporter substrate-binding protein -
  MRY16398_RS04375 (MRY16398_08670) hemW 905373..906509 (-) 1137 WP_039078025.1 radical SAM family heme chaperone HemW -
  MRY16398_RS04380 (MRY16398_08680) - 906502..907095 (-) 594 WP_039078024.1 XTP/dITP diphosphatase -
  MRY16398_RS04385 (MRY16398_08690) yggU 907104..907394 (-) 291 WP_125123887.1 DUF167 family protein YggU -
  MRY16398_RS04390 (MRY16398_08700) - 907391..907954 (-) 564 WP_071195982.1 YggT family protein -
  MRY16398_RS04395 (MRY16398_08710) - 907973..908677 (-) 705 WP_125123888.1 YggS family pyridoxal phosphate-dependent enzyme -
  MRY16398_RS04400 (MRY16398_08720) pilT 908694..909674 (+) 981 WP_125123889.1 type IV pilus twitching motility protein PilT Machinery gene
  MRY16398_RS04405 (MRY16398_08730) - 909671..911929 (-) 2259 WP_125123890.1 FAD-dependent oxidoreductase -
  MRY16398_RS04410 (MRY16398_08740) - 912001..912693 (+) 693 WP_039078018.1 hypothetical protein -
  MRY16398_RS04415 (MRY16398_08750) ruvX 912840..913256 (-) 417 WP_039078017.1 Holliday junction resolvase RuvX -
  MRY16398_RS04420 (MRY16398_08760) - 913256..913819 (-) 564 WP_039078016.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35855.96 Da        Isoelectric Point: 6.6320

>NTDB_id=70686 MRY16398_RS04400 WP_125123889.1 908694..909674(+) (pilT) [Phytobacter sp. MRY16-398]
MDMEKIVELSVKHNVSDLHLCDRRPPRWRRQGRLEAAPFDAVDIHALLAESLNTQQQQQWQACGQIDFALSTPGLQRLRA
SAFLHHEGVSLALRLLPDTCPTLDMLDVPAVIPELLRRDNGLILVTGATGSGKSTTLAAMVDNLNHYLDGHILTLEDPVE
FIHTSKRCLIQQREIGPHCASFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAPQAIERLVDS
FPAEEKEPVRSQLAGSLQAVLSQRLAADRQGGRVALFELLINTPAIANLIREGKNHQLPGVLQTGQQAGMQTFDQSRQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=70686 MRY16398_RS04400 WP_125123889.1 908694..909674(+) (pilT) [Phytobacter sp. MRY16-398]
ATGGATATGGAAAAAATAGTGGAGCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGCGATCGCCGTCCACCACG
GTGGCGAAGGCAAGGACGGCTGGAAGCCGCACCGTTCGACGCGGTGGATATTCACGCGCTGCTCGCCGAAAGTTTAAACA
CGCAGCAGCAACAGCAGTGGCAGGCGTGCGGACAGATAGATTTTGCCCTCAGTACCCCAGGGCTTCAACGCCTTCGCGCC
AGTGCGTTTTTACATCACGAAGGGGTGTCGCTTGCGCTGCGACTGTTGCCTGACACCTGCCCGACGCTGGATATGCTGGA
TGTGCCCGCCGTTATCCCTGAATTACTAAGGCGCGATAACGGTCTTATTCTGGTTACCGGGGCGACAGGCAGCGGTAAAT
CCACCACGCTGGCGGCGATGGTGGATAACCTTAATCACTATCTCGACGGGCATATCCTGACCCTGGAAGATCCCGTGGAG
TTTATCCATACGAGTAAACGTTGCCTGATCCAACAGCGGGAAATCGGCCCGCACTGCGCCTCGTTTGCGGCGGGGTTACG
CGCGGCATTACGCGAAGACCCGGATGTCATTCTGCTGGGGGAATTGCGTGACAGCGAGACCATCCGCCTGGCGTTGACCG
CCGCCGAAACCGGGCATCTGGTGCTGGCAACATTACATACACGCGGCGCACCGCAGGCTATTGAACGGCTGGTGGACAGT
TTCCCCGCCGAGGAGAAAGAGCCGGTGCGTAGCCAGCTTGCCGGAAGCCTGCAGGCCGTATTGTCCCAGCGGCTGGCGGC
AGACAGGCAGGGCGGACGTGTGGCGCTGTTTGAGCTGCTTATCAACACACCTGCGATAGCCAACCTTATCCGTGAAGGGA
AAAACCACCAGTTGCCCGGCGTGTTACAGACCGGGCAGCAGGCGGGAATGCAGACCTTCGACCAAAGCCGCCAGCAACGG
CAGGCGCAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A348D8J9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Pseudomonas aeruginosa PAK

48.318

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Legionella pneumophila strain ERS1305867

47.484

97.546

0.463
  pilT Legionella pneumophila strain Lp02

47.484

97.546

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.34

97.546

0.433
  pilU Vibrio cholerae strain A1552

40.373

98.773

0.399
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

38.7

99.08

0.383

Multiple sequence alignment