Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NLX84_RS20985 Genome accession   NZ_CP100765
Coordinates   4418047..4419069 (-) Length   340 a.a.
NCBI ID   WP_135638616.1    Uniprot ID   -
Organism   Serratia nematodiphila strain SASK3000     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4413047..4424069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLX84_RS20955 (NLX84_20955) - 4413848..4414360 (+) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -
  NLX84_RS20960 (NLX84_20960) endA 4414462..4415157 (+) 696 WP_015379043.1 deoxyribonuclease I -
  NLX84_RS20965 (NLX84_20965) rsmE 4415227..4415958 (+) 732 WP_033632097.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NLX84_RS20970 (NLX84_20970) gshB 4415969..4416919 (+) 951 WP_135638614.1 glutathione synthase -
  NLX84_RS20975 (NLX84_20975) - 4417065..4417628 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  NLX84_RS20980 (NLX84_20980) ruvX 4417628..4418050 (+) 423 WP_033632095.1 Holliday junction resolvase RuvX -
  NLX84_RS20985 (NLX84_20985) pilT 4418047..4419069 (-) 1023 WP_135638616.1 type IV pilus twitching motility protein PilT Machinery gene
  NLX84_RS20990 (NLX84_20990) - 4419090..4419797 (+) 708 WP_015379048.1 YggS family pyridoxal phosphate-dependent enzyme -
  NLX84_RS20995 (NLX84_20995) proC 4419817..4420638 (+) 822 WP_135638618.1 pyrroline-5-carboxylate reductase -
  NLX84_RS21000 (NLX84_21000) - 4420670..4421224 (+) 555 WP_033632091.1 YggT family protein -
  NLX84_RS21005 (NLX84_21005) yggU 4421221..4421514 (+) 294 WP_016930091.1 DUF167 family protein YggU -
  NLX84_RS21010 (NLX84_21010) - 4421655..4422248 (+) 594 WP_016930092.1 XTP/dITP diphosphatase -
  NLX84_RS21015 (NLX84_21015) hemW 4422241..4423383 (+) 1143 WP_033632089.1 radical SAM family heme chaperone HemW -
  NLX84_RS21020 (NLX84_21020) - 4423421..4423855 (-) 435 WP_019456096.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36357.64 Da        Isoelectric Point: 7.5975

>NTDB_id=706798 NLX84_RS20985 WP_135638616.1 4418047..4419069(-) (pilT) [Serratia nematodiphila strain SASK3000]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGVSLALRRIAGQSPSLAKLAAPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHHSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAAEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=706798 NLX84_RS20985 WP_135638616.1 4418047..4419069(-) (pilT) [Serratia nematodiphila strain SASK3000]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCATTGGCGGCGGCGCCAACGCTGACGGCGCAGGGCGCGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACAGGCCGAGCGGGGAA
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGAGTTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCAAGCTGGCGGCGCCAGCCATCGTCCCGGCGTTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCATATTTTGACGCTGGAA
GATCCGATCGAATTCCTGCATCACAGCCGGCGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGTTGCGCGATACCGCCACTATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGGGCCCAGCTGGCCGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCTGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCGGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGAGTGCGCGGCGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

50.755

97.353

0.494
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Neisseria gonorrhoeae MS11

49.405

98.824

0.488
  pilT Neisseria meningitidis 8013

49.849

97.353

0.485
  pilT Legionella pneumophila strain ERS1305867

48.955

98.529

0.482
  pilT Legionella pneumophila strain Lp02

48.955

98.529

0.482
  pilT Pseudomonas stutzeri DSM 10701

47.917

98.824

0.474
  pilT Pseudomonas aeruginosa PAK

48.788

97.059

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.029

100

0.453
  pilU Acinetobacter baylyi ADP1

40.248

95

0.382
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.279

99.118

0.379