Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NLX81_RS21515 Genome accession   NZ_CP100764
Coordinates   4532067..4533077 (-) Length   336 a.a.
NCBI ID   WP_013814483.1    Uniprot ID   A0A7U4DYJ4
Organism   Serratia plymuthica strain SASK2000     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4527067..4538077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLX81_RS21485 (NLX81_21485) - 4527647..4528159 (+) 513 WP_013814477.1 SprT family zinc-dependent metalloprotease -
  NLX81_RS21490 (NLX81_21490) endA 4528367..4529062 (+) 696 WP_013814478.1 deoxyribonuclease I -
  NLX81_RS21495 (NLX81_21495) rsmE 4529247..4529978 (+) 732 WP_013814479.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NLX81_RS21500 (NLX81_21500) gshB 4529989..4530939 (+) 951 WP_013814480.1 glutathione synthase -
  NLX81_RS21505 (NLX81_21505) - 4531073..4531636 (+) 564 WP_013814481.1 YqgE/AlgH family protein -
  NLX81_RS21510 (NLX81_21510) ruvX 4531636..4532058 (+) 423 WP_013814482.1 Holliday junction resolvase RuvX -
  NLX81_RS21515 (NLX81_21515) pilT 4532067..4533077 (-) 1011 WP_013814483.1 type IV pilus twitching motility protein PilT Machinery gene
  NLX81_RS21520 (NLX81_21520) - 4533098..4533805 (+) 708 WP_013814484.1 YggS family pyridoxal phosphate-dependent enzyme -
  NLX81_RS21525 (NLX81_21525) proC 4533954..4534772 (+) 819 WP_254754701.1 pyrroline-5-carboxylate reductase -
  NLX81_RS21530 (NLX81_21530) - 4534804..4535358 (+) 555 WP_004948012.1 YggT family protein -
  NLX81_RS21535 (NLX81_21535) yggU 4535355..4535645 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  NLX81_RS21540 (NLX81_21540) - 4535663..4536256 (+) 594 WP_041416652.1 XTP/dITP diphosphatase -
  NLX81_RS21545 (NLX81_21545) hemW 4536249..4537388 (+) 1140 WP_013814487.1 radical SAM family heme chaperone HemW -
  NLX81_RS21550 (NLX81_21550) - 4537442..4537690 (-) 249 WP_013814488.1 DUF2442 domain-containing protein -
  NLX81_RS21555 (NLX81_21555) - 4537674..4537916 (-) 243 WP_013814489.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 36536.99 Da        Isoelectric Point: 6.6174

>NTDB_id=706775 NLX81_RS21515 WP_013814483.1 4532067..4533077(-) (pilT) [Serratia plymuthica strain SASK2000]
MDIDEFVALSVKHNASDLHLCAGHQPMLRIDGELQPLERAEILTQERMHQLCGALLQPPQRQHLQQHGQLDLALMLAGDI
RLRANFFLQSLGISIALRLIAGHCPTLATLAAPAIVPALLRREDGLILVTGATGSGKSTTLAAMIDDINQHQRRHVITLE
DPIEFMHRSRKSLIQQRELGRDSHSFDEALRAALREDPDVILLGELRDTTTIRLALTAAETGHLVLATLHTRSAPQAVDR
LVDVFPAEEKPYVRAQLAASLQAVIAQKLLSRPGGGRVAIFEVLTATAAVSSMIREGKTHQLSSVLQTGAQSGMQTFEQG
LQQRIDAGLLGGEEGA

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=706775 NLX81_RS21515 WP_013814483.1 4532067..4533077(-) (pilT) [Serratia plymuthica strain SASK2000]
ATGGATATCGACGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCGGGCCATCAGCCCAT
GTTGCGCATTGATGGTGAACTGCAACCGCTGGAAAGGGCGGAAATACTGACTCAGGAGCGAATGCACCAGCTGTGCGGCG
CGCTGCTGCAACCACCGCAACGGCAACATTTGCAGCAGCATGGGCAGCTCGATCTGGCGTTGATGCTGGCGGGCGATATC
CGGCTGCGCGCCAATTTCTTTTTGCAAAGCCTGGGGATATCCATCGCGCTGCGGTTGATTGCCGGCCACTGCCCGACGCT
GGCGACGTTAGCGGCGCCGGCCATTGTCCCCGCGCTGCTGCGGCGTGAGGATGGCCTGATATTGGTCACCGGCGCGACCG
GCAGCGGTAAATCCACCACGCTGGCGGCGATGATTGACGACATCAATCAGCATCAACGTCGGCATGTCATCACGCTGGAA
GACCCGATCGAATTTATGCACCGCAGCCGGAAGTCGTTGATTCAACAGCGTGAACTGGGCCGTGACAGCCACAGTTTTGA
CGAAGCGCTACGCGCCGCGCTGCGTGAAGATCCGGATGTGATCCTGTTGGGAGAACTGCGTGACACCACCACCATCCGTC
TGGCGCTCACTGCGGCGGAAACCGGCCATTTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTGGATCGT
TTGGTAGACGTTTTCCCGGCGGAAGAAAAGCCTTATGTGCGTGCACAGCTGGCTGCCAGCCTGCAGGCAGTGATTGCGCA
AAAGCTGCTAAGCAGGCCTGGTGGTGGGCGGGTGGCAATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAGCATGA
TCCGCGAAGGCAAAACCCATCAGCTCTCCAGCGTGCTGCAAACCGGCGCGCAGTCCGGCATGCAGACTTTCGAACAGGGG
TTACAGCAGCGGATCGACGCCGGTTTGCTGGGTGGAGAAGAAGGGGCGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U4DYJ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.293

99.405

0.53
  pilT Vibrio cholerae strain A1552

53.293

99.405

0.53
  pilT Neisseria meningitidis 8013

49.701

99.405

0.494
  pilT Neisseria gonorrhoeae MS11

49.401

99.405

0.491
  pilT Acinetobacter baylyi ADP1

49.547

98.512

0.488
  pilT Acinetobacter baumannii D1279779

49.697

98.214

0.488
  pilT Acinetobacter baumannii strain A118

49.697

98.214

0.488
  pilT Acinetobacter nosocomialis M2

49.697

98.214

0.488
  pilT Pseudomonas aeruginosa PAK

48.083

100

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.182

98.214

0.473
  pilT Legionella pneumophila strain ERS1305867

47.321

100

0.473
  pilT Legionella pneumophila strain Lp02

47.321

100

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.029

100

0.458
  pilU Acinetobacter baylyi ADP1

38.7

96.131

0.372
  pilU Pseudomonas stutzeri DSM 10701

37.92

97.321

0.369
  pilU Vibrio cholerae strain A1552

37.654

96.429

0.363