Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NLX77_RS01785 Genome accession   NZ_CP100753
Coordinates   367527..368549 (+) Length   340 a.a.
NCBI ID   WP_015379047.1    Uniprot ID   -
Organism   Serratia marcescens strain SASK1000     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 362527..373549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLX77_RS01745 (NLX77_01745) - 362827..363069 (+) 243 WP_004937418.1 DUF4160 domain-containing protein -
  NLX77_RS01750 (NLX77_01750) - 363053..363301 (+) 249 WP_004937422.1 DUF2442 domain-containing protein -
  NLX77_RS01755 (NLX77_01755) hemW 363338..364480 (-) 1143 WP_049189058.1 radical SAM family heme chaperone HemW -
  NLX77_RS01760 (NLX77_01760) - 364473..365066 (-) 594 WP_004937429.1 XTP/dITP diphosphatase -
  NLX77_RS01765 (NLX77_01765) yggU 365085..365375 (-) 291 WP_004948014.1 DUF167 family protein YggU -
  NLX77_RS01770 (NLX77_01770) - 365372..365926 (-) 555 WP_049189057.1 YggT family protein -
  NLX77_RS01775 (NLX77_01775) proC 365958..366779 (-) 822 WP_004937440.1 pyrroline-5-carboxylate reductase -
  NLX77_RS01780 (NLX77_01780) - 366799..367506 (-) 708 WP_049189056.1 YggS family pyridoxal phosphate-dependent enzyme -
  NLX77_RS01785 (NLX77_01785) pilT 367527..368549 (+) 1023 WP_015379047.1 type IV pilus twitching motility protein PilT Machinery gene
  NLX77_RS01790 (NLX77_01790) ruvX 368546..368968 (-) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  NLX77_RS01795 (NLX77_01795) - 368968..369531 (-) 564 Protein_348 YqgE/AlgH family protein -
  NLX77_RS01800 (NLX77_01800) gshB 369677..370627 (-) 951 WP_021504752.1 glutathione synthase -
  NLX77_RS01805 (NLX77_01805) rsmE 370638..371369 (-) 732 WP_015379044.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NLX77_RS01810 (NLX77_01810) endA 371439..372134 (-) 696 WP_118891696.1 deoxyribonuclease I -
  NLX77_RS01815 (NLX77_01815) - 372236..372748 (-) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36417.70 Da        Isoelectric Point: 7.5736

>NTDB_id=706723 NLX77_RS01785 WP_015379047.1 367527..368549(+) (pilT) [Serratia marcescens strain SASK1000]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQPRHVLTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGEGTRG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=706723 NLX77_RS01785 WP_015379047.1 367527..368549(+) (pilT) [Serratia marcescens strain SASK1000]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAAGCGCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACAGGCCGAGCGGGGAA
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGAATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAGTCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACGTTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGATGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCGGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAGGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGTGCGCGGGGGAGGGAACGAGGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Neisseria meningitidis 8013

50.453

97.353

0.491
  pilT Neisseria gonorrhoeae MS11

50.151

97.353

0.488
  pilT Legionella pneumophila strain ERS1305867

49.096

97.647

0.479
  pilT Legionella pneumophila strain Lp02

49.096

97.647

0.479
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Pseudomonas stutzeri DSM 10701

48.485

97.059

0.471
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.539

100

0.456
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.272

95.294

0.365