Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NL732_RS04065 Genome accession   NZ_CP100718
Coordinates   831163..832143 (+) Length   326 a.a.
NCBI ID   WP_001055644.1    Uniprot ID   -
Organism   Salmonella enterica subsp. enterica serovar Weltevreden strain R17.4942     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 826163..837143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NL732_RS04030 (NL732_04030) - 826358..827560 (-) 1203 WP_000081842.1 IS256 family transposase -
  NL732_RS04035 (NL732_04035) - 827619..827747 (+) 129 Protein_791 DUF1202 family protein -
  NL732_RS04040 (NL732_04040) hemW 827839..828975 (-) 1137 WP_001096532.1 radical SAM family heme chaperone HemW -
  NL732_RS04045 (NL732_04045) - 828968..829561 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  NL732_RS04050 (NL732_04050) yggU 829569..829859 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  NL732_RS04055 (NL732_04055) - 829856..830422 (-) 567 WP_001094845.1 YggT family protein -
  NL732_RS04060 (NL732_04060) - 830441..831145 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  NL732_RS04065 (NL732_04065) pilT 831163..832143 (+) 981 WP_001055644.1 type IV pilus twitching motility protein PilT Machinery gene
  NL732_RS04070 (NL732_04070) - 832275..832961 (+) 687 WP_000098337.1 IclR family transcriptional regulator -
  NL732_RS04075 (NL732_04075) ruvX 833008..833424 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  NL732_RS04080 (NL732_04080) - 833424..833987 (-) 564 WP_001053171.1 YqgE/AlgH family protein -
  NL732_RS04085 (NL732_04085) gshB 834203..835150 (-) 948 WP_000593248.1 glutathione synthase -
  NL732_RS04090 (NL732_04090) rsmE 835170..835901 (-) 732 WP_001675718.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NL732_RS04095 (NL732_04095) endA 835978..836685 (-) 708 WP_000286115.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36139.31 Da        Isoelectric Point: 7.5356

>NTDB_id=706444 NL732_RS04065 WP_001055644.1 831163..832143(+) (pilT) [Salmonella enterica subsp. enterica serovar Weltevreden strain R17.4942]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQEGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=706444 NL732_RS04065 WP_001055644.1 831163..832143(+) (pilT) [Salmonella enterica subsp. enterica serovar Weltevreden strain R17.4942]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACCACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCTGTACTATCGCAAAAACTGGAGGT
CGACAGGCAGGAGGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362
  pilU Pseudomonas stutzeri DSM 10701

35.758

100

0.362