Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NL735_RS03960 Genome accession   NZ_CP100707
Coordinates   816095..817075 (+) Length   326 a.a.
NCBI ID   WP_001055649.1    Uniprot ID   A0A8F2UXN2
Organism   Salmonella enterica subsp. enterica serovar Typhimurium strain R18.0409     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 811095..822075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NL735_RS03930 (NL735_03930) - 811673..812680 (+) 1008 WP_000252197.1 DUF1202 domain-containing protein -
  NL735_RS03935 (NL735_03935) hemW 812771..813907 (-) 1137 WP_254535041.1 radical SAM family heme chaperone HemW -
  NL735_RS03940 (NL735_03940) - 813900..814493 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  NL735_RS03945 (NL735_03945) yggU 814501..814791 (-) 291 WP_001277201.1 DUF167 family protein YggU -
  NL735_RS03950 (NL735_03950) - 814788..815354 (-) 567 WP_001094848.1 YggT family protein -
  NL735_RS03955 (NL735_03955) - 815373..816077 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  NL735_RS03960 (NL735_03960) pilT 816095..817075 (+) 981 WP_001055649.1 type IV pilus twitching motility protein PilT Machinery gene
  NL735_RS03965 (NL735_03965) - 817207..817893 (+) 687 WP_000098329.1 IclR family transcriptional regulator -
  NL735_RS03970 (NL735_03970) ruvX 817940..818356 (-) 417 WP_000017096.1 Holliday junction resolvase RuvX -
  NL735_RS03975 (NL735_03975) - 818356..818919 (-) 564 WP_001053171.1 YqgE/AlgH family protein -
  NL735_RS03980 (NL735_03980) gshB 819135..820082 (-) 948 WP_000593248.1 glutathione synthase -
  NL735_RS03985 (NL735_03985) rsmE 820102..820833 (-) 732 WP_001222489.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NL735_RS03990 (NL735_03990) endA 820910..821617 (-) 708 WP_000286122.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36150.38 Da        Isoelectric Point: 8.0973

>NTDB_id=706372 NL735_RS03960 WP_001055649.1 816095..817075(+) (pilT) [Salmonella enterica subsp. enterica serovar Typhimurium strain R18.0409]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAKIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=706372 NL735_RS03960 WP_001055649.1 816095..817075(+) (pilT) [Salmonella enterica subsp. enterica serovar Typhimurium strain R18.0409]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGCTGTCCTGATCTGGCGAAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCAGCGTTACGTGAAGATCCTGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

36.556

100

0.371
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362