Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NLZ15_RS03520 Genome accession   NZ_CP100494
Coordinates   736741..737718 (+) Length   325 a.a.
NCBI ID   WP_254487722.1    Uniprot ID   -
Organism   Atlantibacter subterranea strain LH84-a     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 731741..742718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLZ15_RS03490 (NLZ15_03490) tnpA 732871..733308 (+) 438 WP_254488862.1 IS200/IS605 family transposase -
  NLZ15_RS03495 (NLZ15_03495) hemW 733406..734542 (-) 1137 WP_254487719.1 radical SAM family heme chaperone HemW -
  NLZ15_RS03500 (NLZ15_03500) - 734535..735128 (-) 594 WP_254487720.1 XTP/dITP diphosphatase -
  NLZ15_RS03505 (NLZ15_03505) yggU 735144..735431 (-) 288 WP_125293220.1 DUF167 family protein YggU -
  NLZ15_RS03510 (NLZ15_03510) - 735428..735994 (-) 567 WP_125293221.1 YggT family protein -
  NLZ15_RS03515 (NLZ15_03515) - 736010..736723 (-) 714 WP_254487721.1 YggS family pyridoxal phosphate-dependent enzyme -
  NLZ15_RS03520 (NLZ15_03520) pilT 736741..737718 (+) 978 WP_254487722.1 type IV pilus twitching motility protein PilT Machinery gene
  NLZ15_RS03525 (NLZ15_03525) ruvX 737729..738145 (-) 417 WP_125293224.1 Holliday junction resolvase RuvX -
  NLZ15_RS03530 (NLZ15_03530) - 738145..738708 (-) 564 WP_125293225.1 YqgE/AlgH family protein -
  NLZ15_RS03535 (NLZ15_03535) gshB 738845..739792 (-) 948 WP_144130734.1 glutathione synthase -
  NLZ15_RS03540 (NLZ15_03540) rsmE 739848..740579 (-) 732 WP_125293227.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NLZ15_RS03545 (NLZ15_03545) endA 740654..741361 (-) 708 WP_125293228.1 deoxyribonuclease I -
  NLZ15_RS03550 (NLZ15_03550) - 741458..741967 (-) 510 WP_125293229.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 325 a.a.        Molecular weight: 35319.44 Da        Isoelectric Point: 6.0933

>NTDB_id=705586 NLZ15_RS03520 WP_254487722.1 736741..737718(+) (pilT) [Atlantibacter subterranea strain LH84-a]
MEMEEMVALSVKHNVSDLHLCSDLPARWRRKGALECAPEGVPPPHTLLEQWLSAEQLQIFSRQGQLDFAVTLANGARLRA
NAFQQSQGISLALRILPQTAPQLDTLGAPARLRELLGAQDGLILVTGATGSGKSTTLAAMVNELNRTLSGHILTLEDPIE
YLYTSDKCLIQQREVGTHCDSFSDGLRGALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAVERLVDV
FSAEEKNTIRSQLAGSLKAVLAQKLILTAQGTRSALFELLINTSAVANLIREGKTHQLPGVLQTGQQYGMQSFEQSLQKL
TMMQA

Nucleotide


Download         Length: 978 bp        

>NTDB_id=705586 NLZ15_RS03520 WP_254487722.1 736741..737718(+) (pilT) [Atlantibacter subterranea strain LH84-a]
ATGGAAATGGAAGAAATGGTGGCCCTTAGTGTAAAGCATAATGTTTCGGATCTACACCTGTGCAGCGACTTACCGGCGCG
ATGGCGGCGTAAAGGCGCGCTGGAATGCGCGCCAGAGGGCGTACCACCGCCGCATACGCTGCTTGAGCAGTGGTTATCGG
CTGAGCAGCTGCAGATATTTTCCCGCCAGGGCCAGCTTGATTTTGCAGTCACTCTGGCGAATGGTGCGCGTCTGCGCGCT
AATGCGTTCCAGCAATCTCAGGGGATTTCGCTGGCGCTGCGTATTTTGCCCCAGACCGCGCCACAGCTCGATACGCTGGG
CGCGCCAGCGCGGCTGCGGGAACTGCTTGGCGCTCAGGACGGGCTGATTCTGGTGACCGGCGCGACCGGCAGCGGGAAAT
CCACCACCCTGGCTGCAATGGTCAATGAACTTAACCGAACGTTAAGCGGCCATATTCTGACGCTGGAAGATCCCATTGAA
TATCTTTACACCAGCGACAAGTGCCTGATCCAACAGCGCGAGGTGGGTACCCACTGCGACAGCTTTAGCGACGGGCTGCG
CGGGGCGTTGCGTGAAGATCCTGATGTGATCCTGTTGGGCGAACTGCGCGACAGCGAAACGATCCGGCTGGCATTAACCG
CTGCGGAAACTGGCCATCTGGTGCTGGCGACGCTGCATACCCGCGGCGCGTCCCAGGCGGTTGAACGGCTGGTGGATGTG
TTCAGCGCCGAGGAGAAAAACACAATCCGCAGCCAGCTGGCGGGCAGCCTGAAAGCGGTGCTGGCGCAAAAGCTGATCCT
GACGGCGCAGGGCACGCGGTCAGCGCTGTTTGAACTGCTGATCAACACGTCTGCCGTGGCGAATCTGATCCGGGAAGGCA
AAACCCATCAACTGCCCGGCGTGTTGCAGACCGGGCAGCAGTATGGGATGCAAAGTTTTGAACAGAGCCTGCAAAAGCTG
ACGATGATGCAGGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

48.318

100

0.486
  pilT Neisseria gonorrhoeae MS11

48.012

100

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48

100

0.48
  pilT Vibrio cholerae strain A1552

48

100

0.48
  pilT Pseudomonas stutzeri DSM 10701

46.462

100

0.465
  pilT Legionella pneumophila strain Lp02

46.319

100

0.465
  pilT Legionella pneumophila strain ERS1305867

46.319

100

0.465
  pilT Pseudomonas aeruginosa PAK

46.012

100

0.462
  pilT Acinetobacter baylyi ADP1

44.923

100

0.449
  pilT Acinetobacter baumannii strain A118

44.615

100

0.446
  pilT Acinetobacter baumannii D1279779

44.615

100

0.446
  pilT Acinetobacter nosocomialis M2

44.615

100

0.446
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.331

100

0.425
  pilU Pseudomonas stutzeri DSM 10701

38.58

99.692

0.385
  pilU Vibrio cholerae strain A1552

37.69

100

0.382
  pilU Acinetobacter baylyi ADP1

37.16

100

0.378