Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NJR02_RS18200 Genome accession   NZ_CP100392
Coordinates   3938480..3939514 (+) Length   344 a.a.
NCBI ID   WP_010673050.1    Uniprot ID   A0A081LS93
Organism   Aeromonas caviae strain FAHZZU2447     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3933480..3944514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NJR02_RS18160 (NJR02_18160) - 3933698..3934072 (+) 375 WP_039039964.1 YacL family protein -
  NJR02_RS18165 (NJR02_18165) - 3934122..3934412 (+) 291 WP_039039963.1 hypothetical protein -
  NJR02_RS18170 (NJR02_18170) - 3934574..3935404 (+) 831 WP_104451898.1 CPBP family intramembrane glutamic endopeptidase -
  NJR02_RS18175 (NJR02_18175) - 3935480..3935899 (-) 420 WP_029313762.1 DUF4426 domain-containing protein -
  NJR02_RS18180 (NJR02_18180) yggU 3935918..3936217 (-) 300 WP_010673054.1 DUF167 family protein YggU -
  NJR02_RS18185 (NJR02_18185) - 3936217..3936768 (-) 552 WP_010673053.1 YggT family protein -
  NJR02_RS18190 (NJR02_18190) proC 3936792..3937616 (-) 825 WP_041210938.1 pyrroline-5-carboxylate reductase -
  NJR02_RS18195 (NJR02_18195) - 3937739..3938440 (-) 702 WP_049636471.1 YggS family pyridoxal phosphate-dependent enzyme -
  NJR02_RS18200 (NJR02_18200) pilT 3938480..3939514 (+) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  NJR02_RS18205 (NJR02_18205) pilU 3939538..3940647 (+) 1110 WP_042882153.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NJR02_RS18210 (NJR02_18210) yaaA 3940680..3941453 (+) 774 WP_104451899.1 peroxide stress protein YaaA -
  NJR02_RS18215 (NJR02_18215) srmB 3941641..3942861 (-) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  NJR02_RS18220 (NJR02_18220) - 3942989..3943699 (+) 711 WP_104451900.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38189.90 Da        Isoelectric Point: 6.7436

>NTDB_id=704922 NJR02_RS18200 WP_010673050.1 3938480..3939514(+) (pilT) [Aeromonas caviae strain FAHZZU2447]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHREVHALIYDIMNDHQRKELEENFEVDFSFEVPGMAR
FRVNAFQQARGSGAVFRTIPSTVLTLDDLGAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVDYINDNFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMLGIPAVRNLIREDKVAQLYSVIQTGMTHGMQTMDQSL
KQLVNRGVVASLDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=704922 NJR02_RS18200 WP_010673050.1 3938480..3939514(+) (pilT) [Aeromonas caviae strain FAHZZU2447]
ATGGATATCACAGAGTTATTGGCATTCAGTGTAAAGCATAAGGCCTCGGATCTACACCTCTCGGCCGGGGTCCCCCCGAT
GATCAGGGTTGATGGGGAGGTTCGCAAGATCAATTTGCCCGCCCTGGATCACCGGGAAGTCCATGCTCTCATTTACGACA
TCATGAATGACCACCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTGGACTTCTCCTTCGAAGTGCCCGGCATGGCACGC
TTTCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCGGGCGCCGTGTTTCGTACCATCCCGAGCACCGTTCTGACCCTGGA
CGATCTGGGGGCGCCGGAGATATTTCGCAAGATAGCGGAGTACCCTCGCGGCCTGGTGCTGGTGACTGGGCCCACCGGCT
CGGGCAAATCCACCACCCTGGCCGCCATGGTCGATTACATCAATGACAACTTCCACCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAGAACAAGCGCTGCCTGGTGAACCAGCGGGAAGTACACCGCGACACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGATATCATCCTGGTAGGTGAAATGCGGGACCTCGAAACCATCCGTCTCG
CCATGACCGCCGCCGAAACCGGTCACCTGGTGTTTGGCACCCTGCACACCTCCTCGGCTGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCTCGGAGAAGGACATGGTGCGATCCATGCTCTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGTATCGGTGGCGGCCGGGTGGCGGCCCACGAGATCATGCTGGGGATCCCGGCGGTGCGAAACCTCATCC
GGGAAGACAAGGTGGCCCAGCTCTATTCCGTCATCCAGACCGGGATGACCCACGGCATGCAGACCATGGACCAGAGCCTC
AAGCAGCTGGTCAACCGGGGGGTCGTCGCCTCCCTCGATGCCAAGGCCAAGGCGGTCGATCCCAATACCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A081LS93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Pseudomonas stutzeri DSM 10701

78.299

99.128

0.776
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Pseudomonas aeruginosa PAK

76.833

99.128

0.762
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

71.471

98.837

0.706
  pilT Legionella pneumophila strain ERS1305867

71.471

98.837

0.706
  pilT Neisseria meningitidis 8013

69.027

98.547

0.68
  pilT Neisseria gonorrhoeae MS11

68.732

98.547

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.924

99.419

0.526
  pilU Vibrio cholerae strain A1552

43.188

100

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407