Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EL388_RS13030 Genome accession   NZ_AP018721
Coordinates   2582158..2583204 (-) Length   348 a.a.
NCBI ID   WP_126463816.1    Uniprot ID   A0A4R3JYV1
Organism   Sulfuritortus calidifontis strain J1A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2577158..2588204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL388_RS13005 - 2577982..2578896 (+) 915 WP_126463811.1 CZB domain-containing protein -
  EL388_RS13010 - 2578952..2580013 (-) 1062 WP_126463812.1 Sfum_1244 family protein -
  EL388_RS13015 - 2580089..2580550 (+) 462 WP_126463813.1 EVE domain-containing protein -
  EL388_RS13020 - 2580547..2581341 (+) 795 WP_126463814.1 sulfite exporter TauE/SafE family protein -
  EL388_RS13025 - 2581387..2582121 (+) 735 WP_126463815.1 DUF3530 family protein -
  EL388_RS13030 pilT 2582158..2583204 (-) 1047 WP_126463816.1 type IV pilus twitching motility protein PilT Machinery gene
  EL388_RS13035 - 2583250..2583972 (+) 723 WP_126463817.1 YggS family pyridoxal phosphate-dependent enzyme -
  EL388_RS13040 proC 2583969..2584781 (+) 813 WP_126463818.1 pyrroline-5-carboxylate reductase -
  EL388_RS13045 - 2584781..2585356 (+) 576 WP_126463819.1 YggT family protein -
  EL388_RS13050 - 2585442..2585744 (+) 303 WP_126463820.1 DUF167 domain-containing protein -
  EL388_RS13055 rng 2585752..2587206 (-) 1455 WP_126463821.1 ribonuclease G -
  EL388_RS13060 - 2587265..2587873 (-) 609 WP_126463822.1 Maf family protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38487.21 Da        Isoelectric Point: 6.4194

>NTDB_id=70486 EL388_RS13030 WP_126463816.1 2582158..2583204(-) (pilT) [Sulfuritortus calidifontis strain J1A]
MDISDLLTFVVKNKASDLHLSSGLPPMIRVHGDIRRINLPAMDNNEVRAMIYDIMNDGQRKAYEEHLETDFSFELPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEELGAPEIFKDISNQPRGIVLVTGPTGSGKSTTLAAMVDYINDNMFGHILTIED
PIEFVHQSKKCLINQREVGPHTKGFDEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
VDVFPAAEKDMVRAMLSESLKAVISQSLLKKKDGSGRVAAHEIMIGTPAIRNLIRENKIAQMYSSIQTGQAVGMQTLDQC
LVDLVKRNLVDSEEARPYAVNKDLIPAR

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=70486 EL388_RS13030 WP_126463816.1 2582158..2583204(-) (pilT) [Sulfuritortus calidifontis strain J1A]
ATGGATATCAGCGACCTGCTGACCTTCGTGGTCAAGAACAAGGCCTCCGACTTGCACCTGTCTTCTGGCCTGCCGCCGAT
GATCCGGGTGCACGGCGATATCCGCCGCATCAACCTGCCGGCCATGGACAACAACGAAGTCCGCGCCATGATCTATGACA
TCATGAACGACGGGCAGCGCAAGGCCTACGAAGAGCACCTGGAGACCGACTTCTCCTTCGAGCTGCCCAACGTCGCTCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGGACCATTCCGTCCAAGGTGCTGACCCTGGA
AGAACTCGGCGCGCCGGAGATCTTCAAGGACATCTCCAATCAGCCGCGCGGCATCGTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCGACCACCCTGGCGGCGATGGTCGACTACATCAACGACAACATGTTCGGCCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTCCACCAGAGCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGCCCGCACACCAAGGGCTTCGATGA
GGCGCTGCGCTCGGCCCTGCGGGAAGACCCGGACGTGATCCTGGTCGGCGAGATGCGCGACCTGGAGACCATCCGTCTGG
CGCTGACCGCGGCCGAGACCGGTCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACGGTGGACCGGATC
GTCGACGTCTTCCCGGCGGCGGAGAAGGACATGGTCCGCGCCATGCTGTCGGAATCGCTCAAGGCGGTGATTTCGCAGAG
CCTGCTCAAGAAGAAGGACGGCAGCGGCCGGGTGGCGGCGCACGAGATCATGATCGGCACCCCGGCCATCCGCAACCTGA
TCCGCGAGAACAAGATCGCCCAGATGTATTCCTCGATCCAGACCGGCCAGGCGGTCGGCATGCAGACCCTCGACCAGTGC
CTGGTCGACCTGGTCAAGCGCAATCTGGTGGATTCGGAAGAGGCGCGGCCCTACGCGGTGAACAAGGACCTGATCCCCGC
CCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4R3JYV1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.171

97.414

0.761
  pilT Acinetobacter baumannii D1279779

78.171

97.414

0.761
  pilT Acinetobacter baumannii strain A118

78.171

97.414

0.761
  pilT Pseudomonas stutzeri DSM 10701

77.286

97.414

0.753
  pilT Pseudomonas aeruginosa PAK

76.696

97.414

0.747
  pilT Acinetobacter baylyi ADP1

76.696

97.414

0.747
  pilT Neisseria meningitidis 8013

73.178

98.563

0.721
  pilT Neisseria gonorrhoeae MS11

72.886

98.563

0.718
  pilT Legionella pneumophila strain ERS1305867

71.304

99.138

0.707
  pilT Legionella pneumophila strain Lp02

71.304

99.138

0.707
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.75

96.552

0.664
  pilT Vibrio cholerae strain A1552

68.75

96.552

0.664
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.199

97.989

0.511
  pilU Pseudomonas stutzeri DSM 10701

41.298

97.414

0.402
  pilU Acinetobacter baylyi ADP1

40.882

97.701

0.399
  pilU Vibrio cholerae strain A1552

40.896

96.264

0.394

Multiple sequence alignment