Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NLA06_RS07615 Genome accession   NZ_CP100351
Coordinates   1720182..1721264 (-) Length   360 a.a.
NCBI ID   WP_254080496.1    Uniprot ID   -
Organism   Desulfomicrobium sp. ZS1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1715182..1726264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLA06_RS07590 (NLA06_07590) fbp 1715525..1716541 (-) 1017 WP_254080491.1 class 1 fructose-bisphosphatase -
  NLA06_RS07595 (NLA06_07595) - 1716547..1717239 (-) 693 WP_254080492.1 hypothetical protein -
  NLA06_RS07600 (NLA06_07600) - 1717373..1718125 (-) 753 WP_254080493.1 lipopolysaccharide assembly protein LapB -
  NLA06_RS17595 - 1718127..1718408 (-) 282 WP_371877426.1 septum formation initiator family protein -
  NLA06_RS07605 (NLA06_07605) - 1718412..1718996 (-) 585 WP_254080494.1 zinc-ribbon domain-containing protein -
  NLA06_RS07610 (NLA06_07610) - 1718999..1720180 (-) 1182 WP_254080495.1 type IV pilus twitching motility protein PilT -
  NLA06_RS07615 (NLA06_07615) pilT 1720182..1721264 (-) 1083 WP_254080496.1 type IV pilus twitching motility protein PilT Machinery gene
  NLA06_RS07620 (NLA06_07620) pgsA 1721399..1721938 (-) 540 WP_254080497.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  NLA06_RS07625 (NLA06_07625) - 1721945..1722547 (-) 603 WP_254080498.1 lytic transglycosylase domain-containing protein -
  NLA06_RS07630 (NLA06_07630) - 1722567..1723475 (-) 909 WP_254080499.1 Mrp/NBP35 family ATP-binding protein -
  NLA06_RS07635 (NLA06_07635) - 1723591..1724262 (-) 672 WP_254080500.1 protein-L-isoaspartate(D-aspartate) O-methyltransferase -
  NLA06_RS07640 (NLA06_07640) - 1724414..1724845 (+) 432 WP_254080501.1 CBS domain-containing protein -
  NLA06_RS07645 (NLA06_07645) mgtE 1724858..1726192 (-) 1335 WP_254080502.1 magnesium transporter -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40309.68 Da        Isoelectric Point: 6.5976

>NTDB_id=704715 NLA06_RS07615 WP_254080496.1 1720182..1721264(-) (pilT) [Desulfomicrobium sp. ZS1]
MAQIDAFFKMMHELGASDLHLSSGSQPIIRLHGEMQRIKYKFLEHEELKKMLYEITPENKIKTFEESGDVDFSYEVTHLA
RYRANFFLQKRGIGAVFREIPQKILTIEELGLPSILKNLALLPKGLVLVTGPTGSGKSTTLAAIVDYVNKIRKDHILTIE
DPIEFVHEPQSCLINQREVGRDTMSFSAALRGALREDPDIILVGEMRDLETIQLALEAAETGHLVFATLHTISASKTIDR
IIEVFPGDLQGQIRSGLADSLRAIIAQNLFKRIDKPGRVAGIEVLIATPAVRNLIRENKIFQINSVIETGRKFGMQSIDD
AIMKLLTAGIIDPEQAYNKAATKSKFREFLTTPPQDFTEV

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=704715 NLA06_RS07615 WP_254080496.1 1720182..1721264(-) (pilT) [Desulfomicrobium sp. ZS1]
ATGGCTCAAATAGATGCCTTTTTTAAAATGATGCATGAGCTTGGGGCCTCGGATCTTCATCTGTCATCAGGCTCGCAGCC
GATTATCCGCCTGCATGGCGAAATGCAGCGCATCAAATACAAATTTCTTGAGCATGAAGAACTCAAGAAAATGCTTTACG
AAATAACTCCTGAAAATAAGATCAAGACGTTCGAAGAAAGCGGTGATGTGGATTTCTCCTACGAAGTCACTCATCTGGCT
CGCTACCGCGCCAATTTCTTTTTGCAGAAGCGTGGAATCGGAGCAGTTTTTCGAGAAATTCCGCAAAAAATCCTCACCAT
CGAGGAGCTGGGCCTGCCGAGTATCCTGAAGAATTTGGCTTTATTACCCAAAGGCCTCGTATTGGTCACCGGCCCCACGG
GTAGCGGTAAATCCACGACGCTGGCCGCCATTGTCGATTACGTGAACAAGATCCGCAAGGATCATATTCTGACCATCGAA
GACCCCATCGAATTTGTGCACGAACCGCAGAGTTGTCTGATCAATCAGCGTGAAGTCGGCCGGGACACCATGTCTTTCAG
CGCAGCCTTGCGCGGGGCGCTACGCGAGGATCCGGACATCATTCTGGTCGGCGAAATGCGAGACCTCGAAACCATTCAGT
TGGCCCTTGAGGCGGCAGAGACCGGCCATCTGGTTTTTGCGACCTTGCACACCATCTCCGCCTCGAAAACCATCGACCGC
ATCATCGAGGTCTTTCCCGGTGATTTGCAGGGCCAGATTCGTTCCGGTCTCGCCGACTCCCTGCGGGCCATCATTGCGCA
GAACCTTTTCAAGCGCATAGACAAACCCGGGCGCGTTGCCGGCATTGAAGTCCTCATCGCCACCCCTGCCGTGCGAAACC
TGATCCGCGAAAACAAGATTTTTCAGATCAACTCCGTTATCGAGACTGGACGAAAGTTCGGCATGCAGAGCATCGACGAT
GCCATCATGAAGCTTCTCACCGCCGGTATCATCGACCCCGAGCAAGCATACAACAAGGCTGCCACGAAGTCGAAATTCAG
GGAATTTCTCACCACTCCGCCCCAAGATTTCACCGAGGTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

58.133

92.222

0.536
  pilT Acinetobacter baylyi ADP1

58.006

91.944

0.533
  pilT Acinetobacter baumannii strain A118

57.229

92.222

0.528
  pilT Pseudomonas stutzeri DSM 10701

57.229

92.222

0.528
  pilT Acinetobacter nosocomialis M2

57.229

92.222

0.528
  pilT Acinetobacter baumannii D1279779

57.229

92.222

0.528
  pilT Legionella pneumophila strain Lp02

56.495

91.944

0.519
  pilT Legionella pneumophila strain ERS1305867

56.495

91.944

0.519
  pilT Neisseria meningitidis 8013

57.231

90.278

0.517
  pilT Neisseria gonorrhoeae MS11

56.923

90.278

0.514
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

55.422

92.222

0.511
  pilT Vibrio cholerae strain A1552

55.422

92.222

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.105

95.278

0.458
  pilU Pseudomonas stutzeri DSM 10701

40.29

95.833

0.386
  pilU Vibrio cholerae strain A1552

37.571

98.333

0.369
  pilU Acinetobacter baylyi ADP1

38.596

95

0.367