Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VRUMOI_RS10530 Genome accession   NZ_AP018685
Coordinates   2312921..2313355 (+) Length   144 a.a.
NCBI ID   WP_089138447.1    Uniprot ID   -
Organism   Vibrio rumoiensis strain FERM P-14531     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2307921..2318355
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VRUMOI_RS10515 pdhR 2309488..2310255 (-) 768 WP_089138450.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  VRUMOI_RS10520 ampD 2310716..2311264 (-) 549 WP_089138449.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VRUMOI_RS10525 nadC 2311487..2312407 (+) 921 WP_089138448.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VRUMOI_RS10530 pilA 2312921..2313355 (+) 435 WP_089138447.1 pilin Machinery gene
  VRUMOI_RS19600 - 2313371..2313766 (+) 396 WP_089138446.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VRUMOI_RS10540 pilB 2313806..2315494 (+) 1689 WP_089138445.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VRUMOI_RS10545 pilC 2315563..2316795 (+) 1233 WP_089138444.1 type II secretion system F family protein Machinery gene
  VRUMOI_RS10550 pilD 2316838..2317731 (+) 894 WP_089138443.1 A24 family peptidase Machinery gene

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14989.15 Da        Isoelectric Point: 4.8010

>NTDB_id=70373 VRUMOI_RS10530 WP_089138447.1 2312921..2313355(+) (pilA) [Vibrio rumoiensis strain FERM P-14531]
MKQLQQGKKKLQKGFTLIELMIVVAVIGVLAAIAIPQYQNYVKKAALGSALATATALKTNVEDTIAIKGTFPNNTLSFAL
GSLKLTPTASAADGSIIATVDEGPAQNSTLTLERDADGIWTCKVEGDSVNDEVTPSACEFSSGS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=70373 VRUMOI_RS10530 WP_089138447.1 2312921..2313355(+) (pilA) [Vibrio rumoiensis strain FERM P-14531]
ATGAAACAATTACAACAAGGTAAGAAGAAACTACAAAAAGGTTTTACGCTAATTGAATTGATGATCGTAGTTGCGGTTAT
CGGTGTGCTGGCGGCGATTGCGATTCCTCAGTATCAAAACTATGTGAAAAAAGCCGCTTTAGGTTCTGCTTTGGCGACGG
CAACGGCATTAAAGACCAATGTAGAAGATACAATTGCAATTAAAGGTACATTTCCAAATAACACACTTTCTTTCGCACTT
GGAAGTCTTAAGCTAACACCAACCGCATCAGCTGCCGATGGCTCAATCATCGCAACAGTAGATGAGGGTCCTGCACAAAA
TTCAACTCTAACGTTAGAGCGTGATGCTGATGGTATTTGGACGTGTAAAGTTGAAGGAGATTCAGTCAATGATGAAGTAA
CTCCATCTGCGTGTGAGTTTTCATCTGGCTCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(44-138)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae strain A1552

54.483

100

0.549
  pilA Vibrio cholerae C6706

54.483

100

0.549
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

54.483

100

0.549
  pilA Pseudomonas aeruginosa PAK

41.333

100

0.431
  pilA Vibrio campbellii strain DS40M4

39.61

100

0.424
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.238

87.5

0.396
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.602

92.361

0.375
  comP Acinetobacter baylyi ADP1

36.986

100

0.375
  pilA2 Legionella pneumophila strain ERS1305867

37.241

100

0.375
  pilA2 Legionella pneumophila str. Paris

36.552

100

0.368
  pilA Acinetobacter baumannii strain A118

38.686

95.139

0.368
  pilA Vibrio parahaemolyticus RIMD 2210633

42.063

87.5

0.368
  pilA/pilA1 Eikenella corrodens VA1

34.437

100

0.361

Multiple sequence alignment