Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VRUMOI_RS10540 Genome accession   NZ_AP018685
Coordinates   2313806..2315494 (+) Length   562 a.a.
NCBI ID   WP_089138445.1    Uniprot ID   -
Organism   Vibrio rumoiensis strain FERM P-14531     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2308806..2320494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VRUMOI_RS10515 pdhR 2309488..2310255 (-) 768 WP_089138450.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  VRUMOI_RS10520 ampD 2310716..2311264 (-) 549 WP_089138449.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VRUMOI_RS10525 nadC 2311487..2312407 (+) 921 WP_089138448.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VRUMOI_RS10530 pilA 2312921..2313355 (+) 435 WP_089138447.1 pilin Machinery gene
  VRUMOI_RS19600 - 2313371..2313766 (+) 396 WP_089138446.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VRUMOI_RS10540 pilB 2313806..2315494 (+) 1689 WP_089138445.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VRUMOI_RS10545 pilC 2315563..2316795 (+) 1233 WP_089138444.1 type II secretion system F family protein Machinery gene
  VRUMOI_RS10550 pilD 2316838..2317731 (+) 894 WP_089138443.1 A24 family peptidase Machinery gene
  VRUMOI_RS10555 coaE 2317735..2318358 (+) 624 WP_089138442.1 dephospho-CoA kinase -
  VRUMOI_RS10560 zapD 2318404..2319135 (+) 732 WP_089138441.1 cell division protein ZapD -
  VRUMOI_RS10565 yacG 2319180..2319383 (+) 204 WP_089138440.1 DNA gyrase inhibitor YacG -
  VRUMOI_RS10570 mutT 2319459..2319875 (-) 417 WP_089138439.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62085.29 Da        Isoelectric Point: 5.5470

>NTDB_id=70374 VRUMOI_RS10540 WP_089138445.1 2313806..2315494(+) (pilB) [Vibrio rumoiensis strain FERM P-14531]
MNTNLASILRQAELLTTEQELMVVSTVLNDKKSVPVACVDAGFISSDQLLVKLCHLFALPQANLEHYSFAQVCEQLGMRD
LIITHQVLPLQVDSHSVLLAVADPTNTNVEDDFRFATGKQIEFILADCKELEGAIRRLYGRAVNSDTTQGKDITQDELAN
LVEVGSDEIQETEDLSQDEAPVSRFINQILLDAVRKGASDIHFEPYEDIYRVRLRCDGMLVETNRPAPHLGRRLAARIKI
LSKLDIAERRLPQDGRIKIRLNADTAIDMRVSTLPTLWGEKIVLRLLDSSAANLNIDKLGYSEAQKQLYLDALQQPQGMI
LMTGPTGSGKTVSLYTGLRILNTPDKNISTAEDPVEINLTGINQVQVKPKIGFTFAAALRAFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSSAETVVRLSNMGIEAFNLASSLSLIIAQRLARRLCNHCKKPDDFPEVLREKFHISPDV
VIYKSSKQGCPECTGGYSGRVGIYEVMPFTTELASAVIKKASANDIEMIAVKQGMATLRQSGIENLKQGVTSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=70374 VRUMOI_RS10540 WP_089138445.1 2313806..2315494(+) (pilB) [Vibrio rumoiensis strain FERM P-14531]
ATGAACACCAACCTCGCTTCCATATTACGCCAAGCGGAGCTTTTGACTACAGAGCAAGAGCTTATGGTGGTGAGTACGGT
TTTAAATGATAAAAAAAGCGTCCCCGTGGCTTGCGTGGACGCTGGTTTTATTTCTAGCGATCAATTGCTAGTTAAGCTTT
GCCACTTATTTGCCCTCCCGCAAGCCAACCTTGAACATTACAGCTTTGCTCAAGTGTGTGAACAACTGGGAATGCGCGAT
TTAATCATTACCCACCAAGTGCTGCCGCTACAAGTGGATTCTCATAGCGTATTACTCGCGGTAGCGGACCCGACCAATAC
TAATGTGGAGGACGATTTTCGTTTCGCAACCGGTAAACAAATCGAGTTTATTCTTGCCGATTGTAAAGAATTAGAAGGGG
CAATTCGCCGTTTGTATGGCCGAGCGGTCAACTCGGATACCACTCAAGGCAAAGATATCACCCAAGATGAACTGGCGAAC
TTAGTTGAAGTTGGCAGTGACGAAATCCAAGAAACTGAAGATCTCAGCCAAGATGAAGCGCCGGTCAGCCGTTTCATCAA
CCAAATATTACTCGATGCAGTACGTAAAGGCGCTTCAGATATTCACTTTGAACCTTATGAAGATATCTACCGAGTCCGTT
TGCGTTGCGATGGTATGTTAGTTGAAACCAACCGCCCTGCGCCGCATTTAGGTCGACGCCTTGCCGCGAGGATCAAGATC
CTATCCAAACTGGATATTGCGGAACGTCGCCTGCCGCAAGATGGACGGATCAAGATCCGACTCAATGCTGATACCGCGAT
CGATATGAGAGTTTCAACCTTGCCGACATTGTGGGGAGAGAAGATCGTATTGCGCTTACTAGACAGTAGTGCAGCCAATC
TTAACATTGATAAGCTCGGTTACAGCGAAGCGCAAAAACAATTATATCTAGATGCCCTGCAACAACCACAAGGCATGATC
TTAATGACCGGCCCAACCGGTAGTGGTAAAACCGTTTCGCTTTATACTGGCCTACGAATTCTCAACACTCCCGATAAAAA
TATATCGACCGCCGAAGACCCGGTCGAAATTAACTTAACCGGCATCAATCAAGTACAAGTCAAACCTAAGATTGGCTTTA
CCTTCGCAGCGGCTTTAAGAGCTTTTTTACGTCAAGATCCCGATGTAGTGATGGTCGGTGAGATCCGCGATTTAGAGACA
GCCGAAATTGCGGTAAAAGCAGCGCAGACTGGCCACTTAGTGCTTTCAACTTTACATACTAACTCTTCTGCCGAAACCGT
GGTTCGTCTATCCAATATGGGGATTGAAGCATTTAACTTAGCTTCATCGCTGAGCTTGATCATTGCGCAACGTTTGGCGC
GTCGTTTATGCAATCACTGTAAGAAACCCGATGACTTTCCTGAAGTGTTACGTGAAAAATTTCATATCTCGCCCGATGTA
GTGATTTATAAATCGTCGAAACAAGGTTGTCCGGAATGCACTGGCGGTTATTCAGGTCGAGTGGGGATTTATGAAGTGAT
GCCATTTACCACTGAATTGGCCAGTGCTGTCATCAAAAAAGCCTCTGCCAATGACATTGAAATGATCGCGGTAAAACAAG
GTATGGCGACGCTACGCCAATCAGGAATAGAAAACTTAAAGCAAGGCGTCACCAGCTTTGCAGAATTGCAGCGTGTGCTG
TACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

69.395

100

0.694
  pilB Vibrio parahaemolyticus RIMD 2210633

67.972

100

0.68
  pilB Vibrio campbellii strain DS40M4

67.26

100

0.673
  pilB Legionella pneumophila strain ERS1305867

49.714

93.416

0.464
  pilB Acinetobacter baumannii D1279779

50.881

90.925

0.463
  pilB Acinetobacter baylyi ADP1

51.753

86.299

0.447
  pilF Neisseria gonorrhoeae MS11

43.794

100

0.439

Multiple sequence alignment