Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   VRUMOI_RS10545 Genome accession   NZ_AP018685
Coordinates   2315563..2316795 (+) Length   410 a.a.
NCBI ID   WP_089138444.1    Uniprot ID   -
Organism   Vibrio rumoiensis strain FERM P-14531     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2310563..2321795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VRUMOI_RS10520 ampD 2310716..2311264 (-) 549 WP_089138449.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VRUMOI_RS10525 nadC 2311487..2312407 (+) 921 WP_089138448.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VRUMOI_RS10530 pilA 2312921..2313355 (+) 435 WP_089138447.1 pilin Machinery gene
  VRUMOI_RS19600 - 2313371..2313766 (+) 396 WP_089138446.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VRUMOI_RS10540 pilB 2313806..2315494 (+) 1689 WP_089138445.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VRUMOI_RS10545 pilC 2315563..2316795 (+) 1233 WP_089138444.1 type II secretion system F family protein Machinery gene
  VRUMOI_RS10550 pilD 2316838..2317731 (+) 894 WP_089138443.1 A24 family peptidase Machinery gene
  VRUMOI_RS10555 coaE 2317735..2318358 (+) 624 WP_089138442.1 dephospho-CoA kinase -
  VRUMOI_RS10560 zapD 2318404..2319135 (+) 732 WP_089138441.1 cell division protein ZapD -
  VRUMOI_RS10565 yacG 2319180..2319383 (+) 204 WP_089138440.1 DNA gyrase inhibitor YacG -
  VRUMOI_RS10570 mutT 2319459..2319875 (-) 417 WP_089138439.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 45445.80 Da        Isoelectric Point: 10.2170

>NTDB_id=70375 VRUMOI_RS10545 WP_089138444.1 2315563..2316795(+) (pilC) [Vibrio rumoiensis strain FERM P-14531]
MAQASTLKAPALKNFQWRGVNSSGKKVSGQMLAINEVEVRAKLKEQRVQIKKLKRRSVSIFAKMSHKVKHKDITVFTRQL
ATMLGTGVPIVQAIKLISDSQRKAEMKSVLSQICTQVEAGTPISKAMLASSPFFDEFYCDLVATGEQTGNLADVFDRLAT
YREKSEELRSKVIKAMIYPAMVILVATSVSILMLTMVIPEFEAMFKGFGAELPWFTQQVINLSHFIQSYLFIMILALFGF
IFAMKEARKRSFTFRLKTSRFALQMPVLGGVFSKAAIAKFSRTLATSFSAGIPILTSLLTTAKTSGNLHYQVAIEQVHKD
TAAGMPMYIAMRNADAFPEMVLQMVMIGEESGNLDDMLNKVAAIYEFEVDNTVDNLGKIIEPVIIVFLGVVVGGLVVSLY
LPIFKLVSVF

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=70375 VRUMOI_RS10545 WP_089138444.1 2315563..2316795(+) (pilC) [Vibrio rumoiensis strain FERM P-14531]
ATGGCACAAGCATCCACTCTCAAAGCACCTGCATTAAAAAACTTTCAATGGCGTGGAGTAAATAGCTCTGGCAAAAAAGT
GTCAGGACAAATGTTGGCGATCAACGAAGTTGAGGTGCGCGCTAAACTCAAAGAGCAGCGCGTTCAAATCAAGAAACTCA
AACGACGTAGCGTGTCGATCTTTGCCAAAATGAGCCACAAAGTAAAACATAAAGACATTACAGTATTCACTAGACAGTTG
GCCACGATGCTTGGCACTGGCGTTCCCATCGTTCAGGCGATCAAATTGATCTCAGATAGCCAACGAAAAGCCGAAATGAA
ATCGGTACTCTCGCAGATTTGTACTCAAGTAGAAGCCGGTACGCCAATCTCCAAAGCTATGCTCGCCAGTAGCCCTTTCT
TTGATGAATTCTATTGTGATCTTGTGGCGACAGGTGAGCAAACCGGTAACTTGGCTGATGTGTTTGATCGTCTTGCGACC
TATCGTGAAAAAAGTGAAGAGCTAAGATCAAAAGTAATAAAAGCCATGATCTACCCGGCGATGGTTATTTTAGTTGCCAC
CAGCGTATCAATTCTGATGTTAACCATGGTGATCCCTGAATTTGAAGCCATGTTTAAGGGCTTTGGCGCCGAACTGCCTT
GGTTTACTCAACAAGTGATCAATTTATCTCACTTTATTCAAAGTTATTTATTCATCATGATCCTAGCGCTATTTGGCTTC
ATCTTTGCAATGAAAGAAGCACGTAAACGATCCTTTACTTTTCGACTTAAAACCAGTCGCTTCGCCTTACAAATGCCAGT
ATTAGGCGGCGTTTTTTCCAAAGCGGCGATTGCCAAATTTAGCCGCACATTAGCCACCAGCTTTAGTGCCGGTATTCCTA
TTTTAACGAGTTTATTAACGACCGCTAAAACCTCTGGCAATTTGCATTATCAAGTGGCAATAGAGCAAGTACACAAAGAC
ACCGCCGCGGGCATGCCAATGTACATTGCAATGCGTAACGCCGATGCGTTTCCTGAAATGGTACTGCAAATGGTGATGAT
AGGTGAAGAGTCCGGTAACCTCGATGATATGCTCAATAAGGTCGCAGCAATTTATGAATTTGAAGTGGATAATACCGTCG
ATAACCTAGGTAAAATTATTGAACCGGTGATTATTGTGTTCCTTGGTGTTGTAGTAGGCGGTTTAGTTGTGTCACTTTAT
CTGCCAATCTTTAAGTTAGTTAGTGTATTCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(76-199)

Type II secretion system (T2SS), protein F (T2SSF)

(280-402)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

71.93

97.317

0.7
  pilC Vibrio campbellii strain DS40M4

67.662

98.049

0.663
  pilC Acinetobacter baumannii D1279779

44.169

98.293

0.434
  pilC Acinetobacter baylyi ADP1

43.434

96.585

0.42
  pilC Legionella pneumophila strain ERS1305867

41.147

97.805

0.402
  pilC Pseudomonas stutzeri DSM 10701

40.898

97.805

0.4
  pilG Neisseria meningitidis 44/76-A

40.196

99.512

0.4
  pilG Neisseria gonorrhoeae MS11

40.648

97.805

0.398

Multiple sequence alignment