Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NKF27_RS18815 Genome accession   NZ_CP099965
Coordinates   4345530..4346636 (-) Length   368 a.a.
NCBI ID   WP_301271162.1    Uniprot ID   -
Organism   Halomonas sp. DN3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4340530..4351636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NKF27_RS18805 (NKF27_18805) tyrS 4342986..4344188 (-) 1203 WP_045993280.1 tyrosine--tRNA ligase -
  NKF27_RS18810 (NKF27_18810) - 4344367..4345500 (+) 1134 WP_253554244.1 anhydro-N-acetylmuramic acid kinase -
  NKF27_RS18815 (NKF27_18815) pilT 4345530..4346636 (-) 1107 WP_301271162.1 type IV pilus twitching motility protein PilT Machinery gene
  NKF27_RS18820 (NKF27_18820) - 4346703..4347398 (+) 696 WP_253554245.1 YggS family pyridoxal phosphate-dependent enzyme -
  NKF27_RS18825 (NKF27_18825) proC 4347477..4348301 (+) 825 WP_253554249.1 pyrroline-5-carboxylate reductase -
  NKF27_RS18830 (NKF27_18830) - 4348322..4348915 (+) 594 WP_045993284.1 YggT family protein -
  NKF27_RS18835 (NKF27_18835) - 4348979..4350154 (+) 1176 WP_045993285.1 homoserine O-acetyltransferase -
  NKF27_RS18840 (NKF27_18840) metW 4350144..4350740 (+) 597 WP_045993286.1 methionine biosynthesis protein MetW -
  NKF27_RS18845 (NKF27_18845) - 4350848..4351318 (+) 471 WP_253554251.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40067.54 Da        Isoelectric Point: 6.0517

>NTDB_id=703026 NKF27_RS18815 WP_301271162.1 4345530..4346636(-) (pilT) [Halomonas sp. DN3]
MDITELLALSAKQGASDLHLSAGLPPVMRVNGELERLPGPPMGDGQVRLLLESLMDEELREQYSRTHEADFSHQIPGLSR
YRVNAFVQRRGAAAVLRTIPDRIPTLSELGMGEIFERLVALPRGLVLVTGPTGSGKSTTLAAMIDHINRHRRDHILTIED
PIEFIHHSQQALVSQREVHRHTEGFAVALRSALREDPDVILVGEIRDLETARLAMTAAETGHLVFATLHTASAVKTIDRI
IDAFPGDEKNAVRTMLSESLQAVVSQALLPSSRGGRVAAREILLINQAIRHLIREDKLAQIYSSMQTGGQAGMQTLDADL
ARLVACGEVAPEDARRLTRESLVDSGPSAGWSGGQNEGWVSPQGEGWG

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=703026 NKF27_RS18815 WP_301271162.1 4345530..4346636(-) (pilT) [Halomonas sp. DN3]
ATGGATATTACCGAACTGCTGGCACTCTCGGCAAAGCAGGGCGCCTCGGATCTGCACCTGTCGGCGGGACTGCCACCGGT
GATGCGCGTCAATGGTGAACTGGAGCGTCTGCCTGGCCCGCCGATGGGCGATGGTCAGGTCAGATTGTTGCTCGAGTCGT
TGATGGATGAGGAGCTGCGCGAGCAGTATTCGCGCACCCACGAGGCCGACTTTTCCCACCAGATACCGGGTCTGTCGCGT
TATCGCGTCAATGCTTTCGTCCAGCGGCGCGGGGCGGCGGCGGTATTGCGTACCATCCCCGATCGCATCCCGACGCTTTC
GGAGCTGGGGATGGGCGAGATCTTCGAACGTCTTGTCGCCCTGCCGCGGGGCCTGGTACTGGTGACCGGGCCCACCGGGT
CCGGCAAGAGCACAACGCTTGCGGCGATGATCGACCATATCAACCGCCACCGTCGCGACCATATCCTCACCATCGAGGAT
CCCATCGAGTTCATCCACCACAGCCAGCAGGCGCTGGTGTCCCAGCGAGAGGTACACCGCCACACCGAGGGCTTTGCCGT
CGCCCTGCGCAGCGCCCTGCGCGAGGATCCCGACGTCATTCTGGTCGGAGAGATTCGCGACCTGGAAACGGCGCGCCTGG
CCATGACCGCGGCCGAGACCGGGCATCTGGTATTTGCCACCCTGCATACCGCCTCCGCGGTCAAGACCATCGATCGGATC
ATCGATGCCTTCCCGGGTGATGAAAAGAATGCGGTGCGGACCATGCTGTCGGAGTCCTTGCAGGCGGTTGTCTCCCAGGC
GCTGCTGCCCAGCAGCCGGGGCGGGCGAGTGGCGGCGCGAGAGATCCTCTTGATCAATCAGGCGATCCGTCATCTGATCC
GTGAGGACAAGTTGGCTCAGATTTACTCGTCGATGCAGACCGGCGGCCAGGCCGGCATGCAGACCCTGGACGCCGATCTG
GCGAGACTGGTGGCCTGTGGCGAGGTGGCGCCGGAGGATGCCCGGCGCCTGACCCGTGAATCTCTGGTCGATAGCGGGCC
GAGCGCCGGCTGGAGTGGCGGGCAGAACGAGGGCTGGGTGAGCCCCCAGGGCGAAGGCTGGGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(82-263)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

64.776

91.033

0.59
  pilT Pseudomonas stutzeri DSM 10701

62.941

92.391

0.582
  pilT Acinetobacter baylyi ADP1

62.832

92.12

0.579
  pilT Acinetobacter baumannii strain A118

61.765

92.391

0.571
  pilT Acinetobacter baumannii D1279779

61.765

92.391

0.571
  pilT Acinetobacter nosocomialis M2

61.471

92.391

0.568
  pilT Legionella pneumophila strain Lp02

62.654

88.043

0.552
  pilT Legionella pneumophila strain ERS1305867

62.654

88.043

0.552
  pilT Neisseria meningitidis 8013

58.187

92.935

0.541
  pilT Neisseria gonorrhoeae MS11

57.895

92.935

0.538
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

58.507

91.033

0.533
  pilT Vibrio cholerae strain A1552

58.507

91.033

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.692

88.315

0.457
  pilU Pseudomonas stutzeri DSM 10701

43.582

91.033

0.397
  pilU Vibrio cholerae strain A1552

42.687

91.033

0.389
  pilU Acinetobacter baylyi ADP1

40.476

91.304

0.37