Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NGM44_RS02525 Genome accession   NZ_CP099960
Coordinates   544326..545366 (+) Length   346 a.a.
NCBI ID   WP_253224104.1    Uniprot ID   -
Organism   Moraxella sp. FZFQ2102     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 539326..550366
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGM44_RS02505 (NGM44_02505) - 539967..540599 (+) 633 WP_253224100.1 trimeric intracellular cation channel family protein -
  NGM44_RS02510 (NGM44_02510) - 540733..541695 (+) 963 WP_253224101.1 bile acid:sodium symporter family protein -
  NGM44_RS02515 (NGM44_02515) - 541964..543208 (-) 1245 WP_253224102.1 NnrS family protein -
  NGM44_RS02520 (NGM44_02520) - 543492..544187 (-) 696 WP_253224103.1 YggS family pyridoxal phosphate-dependent enzyme -
  NGM44_RS02525 (NGM44_02525) pilT 544326..545366 (+) 1041 WP_253224104.1 type IV pilus twitching motility protein PilT Machinery gene
  NGM44_RS02530 (NGM44_02530) fur 545431..545865 (-) 435 WP_253224105.1 ferric iron uptake transcriptional regulator -
  NGM44_RS02535 (NGM44_02535) - 546124..546504 (+) 381 WP_253224106.1 outer membrane protein assembly factor BamE -
  NGM44_RS02540 (NGM44_02540) - 546609..546953 (-) 345 WP_253224107.1 RnfH family protein -
  NGM44_RS02545 (NGM44_02545) - 546950..548032 (-) 1083 WP_253224108.1 hypothetical protein -
  NGM44_RS02550 (NGM44_02550) - 548157..548852 (-) 696 WP_253224109.1 cytochrome c -
  NGM44_RS02555 (NGM44_02555) - 549238..549483 (-) 246 WP_371923536.1 cytochrome c5 family protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 37520.99 Da        Isoelectric Point: 6.4455

>NTDB_id=702974 NGM44_RS02525 WP_253224104.1 544326..545366(+) (pilT) [Moraxella sp. FZFQ2102]
MLNLTNLLTFAHAQGASDLHLSTGNVFMRIDGVLTPVALPTPDHQMVSDLLRSVMSDAQYQTWQNELECDFVIEVTGVAR
FRVNAFFQTHGAAAVFRTIASTVPSLDSLFDDEICQILEHISHAKSGLVLVTGSTGSGKSTTLAAMVNAINRTQAAHILT
IEDPIEYLHTSDKSLISQRQVGKDTHSFSHALRSALREDPDVILVGELRDLESIRLALTAAETGHLVLATLHTINAAKSI
DRIIDVFDSHEKNMIRTMLADSLTAIIAQRLLPKTGGGRVAALEILQHTPAVANLIRENKLAQLPSVIQTGQAHGMTSLD
QSLLSLVKQGKISKETANLAAKEMVF

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=702974 NGM44_RS02525 WP_253224104.1 544326..545366(+) (pilT) [Moraxella sp. FZFQ2102]
ATGCTTAACCTAACCAACTTACTCACTTTCGCCCATGCCCAAGGCGCGTCTGATTTGCACTTATCCACCGGCAATGTCTT
TATGCGTATCGATGGCGTGCTTACGCCTGTTGCCCTGCCTACGCCCGATCATCAGATGGTGAGCGATCTGTTGCGCTCGG
TGATGAGTGATGCGCAGTACCAGACCTGGCAAAATGAGCTTGAATGTGATTTTGTCATCGAAGTGACGGGCGTGGCGCGC
TTTCGTGTCAATGCCTTTTTTCAGACGCATGGCGCGGCGGCGGTATTTCGCACCATTGCAAGTACTGTACCAAGTCTAGA
TAGCCTATTTGATGATGAAATTTGTCAGATTCTTGAGCATATCAGCCACGCCAAATCAGGGCTTGTACTGGTCACAGGCA
GCACAGGCTCAGGCAAATCCACCACGCTTGCCGCCATGGTCAATGCCATCAATCGCACGCAAGCTGCACACATTCTCACC
ATCGAAGACCCGATTGAATATCTACACACGAGTGATAAATCTCTCATCAGTCAGCGACAAGTCGGCAAGGATACGCACAG
CTTTAGCCATGCGCTGCGCTCAGCACTGCGTGAAGATCCTGATGTGATTTTGGTCGGTGAACTTCGCGACCTTGAGAGCA
TTCGCCTTGCACTCACCGCTGCTGAGACTGGGCATTTAGTACTGGCGACGCTACACACTATCAATGCCGCCAAGAGCATT
GATCGCATCATCGATGTGTTTGATTCGCATGAGAAAAATATGATTCGCACCATGCTTGCCGACTCGCTTACCGCGATCAT
CGCACAGCGGCTGCTGCCAAAGACAGGTGGTGGACGCGTGGCAGCATTAGAGATTTTGCAGCATACGCCTGCGGTAGCGA
ATTTGATCCGCGAAAATAAGCTTGCGCAGCTACCATCAGTGATCCAAACAGGGCAAGCACACGGTATGACAAGCCTTGAT
CAGTCGCTATTATCACTGGTCAAACAAGGCAAAATCAGTAAAGAGACGGCGAATTTAGCCGCAAAAGAGATGGTGTTTTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

58.892

99.133

0.584
  pilT Acinetobacter baumannii D1279779

58.892

99.133

0.584
  pilT Acinetobacter baumannii strain A118

58.892

99.133

0.584
  pilT Neisseria meningitidis 8013

58.43

99.422

0.581
  pilT Neisseria gonorrhoeae MS11

58.14

99.422

0.578
  pilT Acinetobacter baylyi ADP1

57.31

98.844

0.566
  pilT Pseudomonas stutzeri DSM 10701

56.851

99.133

0.564
  pilT Pseudomonas aeruginosa PAK

56.725

98.844

0.561
  pilT Legionella pneumophila strain Lp02

55.718

98.555

0.549
  pilT Legionella pneumophila strain ERS1305867

55.718

98.555

0.549
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

56.667

95.376

0.54
  pilT Vibrio cholerae strain A1552

56.667

95.376

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.667

95.376

0.445
  pilU Pseudomonas stutzeri DSM 10701

39.474

98.844

0.39
  pilU Vibrio cholerae strain A1552

40.798

94.22

0.384
  pilU Acinetobacter baylyi ADP1

38.209

96.821

0.37