Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NI392_RS21135 Genome accession   NZ_CP099950
Coordinates   2804345..2805385 (-) Length   346 a.a.
NCBI ID   WP_104975598.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain Isc1B     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2799345..2810385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI392_RS21105 (NI392_21255) endA 2799489..2800184 (+) 696 WP_017635582.1 deoxyribonuclease I -
  NI392_RS21110 (NI392_21260) rsmE 2800298..2801029 (+) 732 WP_104975597.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NI392_RS21115 (NI392_21265) gshB 2801043..2801993 (+) 951 WP_005381021.1 glutathione synthase -
  NI392_RS21120 (NI392_21270) - 2802122..2802685 (+) 564 WP_104976024.1 YqgE/AlgH family protein -
  NI392_RS21125 (NI392_21275) ruvX 2802721..2803146 (+) 426 WP_053304143.1 Holliday junction resolvase RuvX -
  NI392_RS21130 (NI392_21280) pilU 2803208..2804314 (-) 1107 WP_005381016.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NI392_RS21135 (NI392_21285) pilT 2804345..2805385 (-) 1041 WP_104975598.1 type IV pilus twitching motility protein PilT Machinery gene
  NI392_RS21140 (NI392_21290) - 2805414..2806115 (+) 702 WP_005384521.1 YggS family pyridoxal phosphate-dependent enzyme -
  NI392_RS21145 (NI392_21295) proC 2806275..2807093 (+) 819 WP_005381013.1 pyrroline-5-carboxylate reductase -
  NI392_RS21150 (NI392_21300) - 2807146..2807703 (+) 558 WP_005381012.1 YggT family protein -
  NI392_RS21155 (NI392_21305) yggU 2807703..2807993 (+) 291 WP_005393980.1 DUF167 family protein YggU -
  NI392_RS21160 (NI392_21310) - 2808122..2808553 (+) 432 WP_104975599.1 DUF4426 domain-containing protein -
  NI392_RS21165 (NI392_21315) - 2808672..2809274 (+) 603 WP_005381009.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38531.04 Da        Isoelectric Point: 6.6198

>NTDB_id=702812 NI392_RS21135 WP_104975598.1 2804345..2805385(-) (pilT) [Vibrio alginolyticus strain Isc1B]
MDITELLNFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVAREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=702812 NI392_RS21135 WP_104975598.1 2804345..2805385(-) (pilT) [Vibrio alginolyticus strain Isc1B]
ATGGATATCACTGAGTTACTGAATTTTAGTGTAAAACATAATGCCTCGGATCTACATCTTTCTGCAGGTGTCCCACCAAT
GGTTCGTATTGATGGGGATGTGAGAAAACTAGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTCGAAA
TCATGAACGATGCCCAACGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAGTTGCATAACGTTGGCCGT
TTCCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCAGTATTTCGTACCATTCCAAGTAGCATTCCAACTCTGGA
AGAGCTAGAAGCGCCAGAGATCTTCAAAAAAATTGCGAACGCTGAAAAAGGCTTAGTTCTGGTCACTGGCCCAACAGGTT
CAGGTAAATCGACGACCTTAGCAGCCATTGTTGACTACATTAACCGTAATCACAACAAGCACGTACTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAATGTTTGATCAACCAGCGTGAAGTTCACCGTGACACCCATAGTTTCCAAAA
TGCTTTGCGTAGTGCATTGCGCGAAGACCCAGACGTTATTCTGGTTGGTGAGATGCGTGACAAAGAAACCATCAGCCTTG
CCTTAACGGCGGCAGAAACGGGTCACTTAGTTTTCGGGACGTTGCACACCAGCTCGGCGGCAAAAACCATTGACCGTATT
ATCGATGTGTTTCCTGGTAGTGATAAAGATATGGTCCGCTCGATGCTATCTGAGTCCCTTCGTTCGGTTATCGCGCAAAA
GCTTCTGAAGCGTAATGGTGGTGGCCGTATTGCATGCCATGAAATTATGATGGCAACGCCTGCGATTCGTAACTTGATCC
GCGAAGACAAAGTCGCGCAGATGTATTCGATTATTCAAACAGGTGCGGCTCACGGCATGCAAACCATGGAGCAAAATGCT
CGTCAGTTAATGGCGCAAGGCATGGTTGCTCGTGAAGAGGTCGATAGTAAGATCGGATTAGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.957

99.711

0.867
  pilT Vibrio cholerae strain A1552

86.957

99.711

0.867
  pilT Acinetobacter baumannii D1279779

72.121

95.376

0.688
  pilT Acinetobacter baumannii strain A118

72.121

95.376

0.688
  pilT Acinetobacter nosocomialis M2

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.429

95.087

0.679
  pilT Pseudomonas aeruginosa PAK

69.139

97.399

0.673
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.399

0.671
  pilT Legionella pneumophila strain Lp02

67.674

95.665

0.647
  pilT Legionella pneumophila strain ERS1305867

67.674

95.665

0.647
  pilT Neisseria meningitidis 8013

65.766

96.243

0.633
  pilT Neisseria gonorrhoeae MS11

65.465

96.243

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.83

91.908

0.486
  pilU Vibrio cholerae strain A1552

41.018

96.532

0.396
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.532

0.393
  pilU Acinetobacter baylyi ADP1

39.375

92.486

0.364