Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NI384_RS02415 Genome accession   NZ_CP099928
Coordinates   497163..498269 (+) Length   368 a.a.
NCBI ID   WP_308385403.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain BM24B     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 492163..503269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI384_RS02380 (NI384_02380) - 492233..492835 (-) 603 WP_005482459.1 XTP/dITP diphosphatase -
  NI384_RS02385 (NI384_02385) - 492953..493384 (-) 432 WP_005461703.1 DUF4426 domain-containing protein -
  NI384_RS02390 (NI384_02390) yggU 493512..493802 (-) 291 WP_005482461.1 DUF167 family protein YggU -
  NI384_RS02395 (NI384_02395) - 493802..494359 (-) 558 WP_005482440.1 YggT family protein -
  NI384_RS02400 (NI384_02400) proC 494412..495230 (-) 819 WP_005482485.1 pyrroline-5-carboxylate reductase -
  NI384_RS02405 (NI384_02405) - 495350..496063 (-) 714 WP_021450047.1 YggS family pyridoxal phosphate-dependent enzyme -
  NI384_RS02410 (NI384_02410) pilT 496092..497132 (+) 1041 WP_005461712.1 type IV pilus twitching motility protein PilT Machinery gene
  NI384_RS02415 (NI384_02415) pilU 497163..498269 (+) 1107 WP_308385403.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NI384_RS02420 (NI384_02420) ruvX 498330..498755 (-) 426 WP_005461715.1 Holliday junction resolvase RuvX -
  NI384_RS02425 (NI384_02425) - 498804..499367 (-) 564 WP_005461717.1 YqgE/AlgH family protein -
  NI384_RS02430 (NI384_02430) gshB 499481..500431 (-) 951 WP_005425823.1 glutathione synthase -
  NI384_RS02435 (NI384_02435) rsmE 500446..501177 (-) 732 WP_005461719.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NI384_RS02440 (NI384_02440) endA 501302..501997 (-) 696 WP_005493878.1 deoxyribonuclease I -
  NI384_RS02445 (NI384_02445) - 502117..502614 (-) 498 WP_015297290.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41484.76 Da        Isoelectric Point: 7.2517

>NTDB_id=702672 NI384_RS02415 WP_308385403.1 497163..498269(+) (pilU) [Vibrio parahaemolyticus strain BM24B]
MDLNKFLEGMLTLKASDLYITVGAPILFRVDGELRPQGEKLTEHDVAKLLDSAMDPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETIIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKIREEDALHSADSANDLRLMLKTQRGEPFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=702672 NI384_RS02415 WP_308385403.1 497163..498269(+) (pilU) [Vibrio parahaemolyticus strain BM24B]
ATGGATCTGAATAAATTTCTTGAAGGTATGTTGACGCTAAAAGCGTCGGATCTTTACATCACGGTTGGTGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAGAAACTCACCGAGCATGATGTTGCAAAGTTGCTTGATAGCGCAA
TGGATCCGGATCGGCGTCAGGAGTTTCGCAAAAGCCGCGAATCAAACTTTGCTATCGTCAGAGATTGTGGTCGCTTCCGT
GTTAGTGCTTTTTTTCAGCGAGAACTGCCGGGGGCAGTTATTCGCCGTATCGAAACCATCATTCCTACTTTTGAGCAGTT
AAAACTGCCTTTAGTGCTGCAAGACTTAGCGATTGCCAAGCGTGGTTTGGTGCTGGTGGTCGGCGCGACTGGCTCGGGTA
AATCGACCACGATGGCGGCGATGACGGGCTATCGAAACAGCAACAAAACCGGTCACATCTTGACGGTCGAAGATCCGATC
GAATTTGTGCATGAGCATAAGCGTTGTATCGTGACTCAGCGTGAGGTGGGTTTGGATACCGAAAGCTATGAAGTCGCACT
GAAAAACTCGTTGCGCCAAGCGCCAGATATGATTTTGATTGGCGAGATCCGTAGCCGAGAAACGATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACTTGTGTATGGCAACGTTGCACGCCAACAACGCCAACCAAGCGTTAGAGCGTATTCTCCAT
TTGGTGCCTAAAGATCAAAAAGAGCAATTCTTGTTTGATTTGTCGATGAACCTAAAAGGCGTGATTGGTCAGCAGCTGAT
CCGTGATAAGAATGGTCAAGGACGTCATGGTGTGTTTGAGATATTGCTAAACAGTCCACGAGTGTCGGATTTGATCCGTC
GTGGTGATTTACACGAATTGAAATCAACCATGGCACGATCCAATGAGTTTGGCATGCTGACGTTTGACCAATCGCTATAC
AAGCTGGTGATGCAGGGTAAGATCAGAGAAGAGGATGCACTGCACAGTGCGGATTCAGCCAACGATCTTCGTTTAATGCT
GAAAACACAGCGTGGTGAGCCGTTCTCTACAGGCAGTCTTGCCAACGTCAAAATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.88

100

0.829
  pilU Pseudomonas stutzeri DSM 10701

58

95.109

0.552
  pilU Acinetobacter baylyi ADP1

54.986

95.38

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.231

88.315

0.399
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Acinetobacter nosocomialis M2

42.183

92.12

0.389
  pilT Acinetobacter baumannii D1279779

42.183

92.12

0.389
  pilT Acinetobacter baumannii strain A118

42.183

92.12

0.389
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

42.121

89.674

0.378
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

89.674

0.375
  pilT Vibrio cholerae strain A1552

41.818

89.674

0.375
  pilT Neisseria gonorrhoeae MS11

40.361

90.217

0.364
  pilT Neisseria meningitidis 8013

40.361

90.217

0.364