NTDB

Overview

Name	pilU	Type	Machinery gene
Locus tag	NI387_RS02420	Genome accession	NZ_CP099921
Coordinates	498492..499598 (+)	Length	368 a.a.
NCBI ID	WP_042771568.1	Uniprot ID	-
Organism	Vibrio parahaemolyticus strain BM25
Function	type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 493492..504598

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NI387_RS02385 (NI387_02385)	-	493562..494164 (-)	603	WP_005482459.1	XTP/dITP diphosphatase	-
NI387_RS02390 (NI387_02390)	-	494282..494713 (-)	432	WP_005461703.1	DUF4426 domain-containing protein	-
NI387_RS02395 (NI387_02395)	yggU	494841..495131 (-)	291	WP_005482461.1	DUF167 family protein YggU	-
NI387_RS02400 (NI387_02400)	-	495131..495688 (-)	558	WP_005482440.1	YggT family protein	-
NI387_RS02405 (NI387_02405)	proC	495741..496559 (-)	819	WP_042771570.1	pyrroline-5-carboxylate reductase	-
NI387_RS02410 (NI387_02410)	-	496679..497392 (-)	714	WP_042771569.1	YggS family pyridoxal phosphate-dependent enzyme	-
NI387_RS02415 (NI387_02415)	pilT	497421..498461 (+)	1041	WP_005461712.1	type IV pilus twitching motility protein PilT	Machinery gene
NI387_RS02420 (NI387_02420)	pilU	498492..499598 (+)	1107	WP_042771568.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NI387_RS02425 (NI387_02425)	ruvX	499660..500085 (-)	426	WP_005461715.1	Holliday junction resolvase RuvX	-
NI387_RS02430 (NI387_02430)	-	500134..500697 (-)	564	WP_005461717.1	YqgE/AlgH family protein	-
NI387_RS02435 (NI387_02435)	gshB	500811..501761 (-)	951	WP_005425823.1	glutathione synthase	-
NI387_RS02440 (NI387_02440)	rsmE	501776..502507 (-)	732	WP_017449132.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
NI387_RS02445 (NI387_02445)	endA	502632..503327 (-)	696	WP_308384749.1	deoxyribonuclease I	-
NI387_RS02450 (NI387_02450)	-	503447..503944 (-)	498	WP_021453096.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

Download Length: 368 a.a. Molecular weight: 41417.73 Da Isoelectric Point: 7.2515

                                >NTDB_id=702587 NI387_RS02420 WP_042771568.1 498492..499598(+) (pilU) [Vibrio parahaemolyticus strain BM25]

MDLNKFLEGMLTLKASDLYITVGAPILFRVDGELRPQGEKLTEHDVAKLLDSAMDPDRRQVFRKSRESNFAIVRDCGRFR

VSAFFQRELPGAVIRRIETIIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI

EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH

LMPKDQKEQFLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY

KLVMQGKISEEDALHSADSANDLRLMLKTQRGEPFSTGSLANVKIDMD

Nucleotide

Download Length: 1107 bp

                                >NTDB_id=702587 NI387_RS02420 WP_042771568.1 498492..499598(+) (pilU) [Vibrio parahaemolyticus strain BM25]

ATGGATCTGAATAAATTTCTTGAAGGTATGTTGACGCTAAAAGCGTCGGATCTTTACATCACGGTTGGTGCGCCAATTCT

ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAGAAACTCACCGAACATGATGTTGCAAAGTTGCTTGATAGCGCAA

TGGATCCGGATCGGCGTCAGGTGTTTCGCAAAAGCCGCGAATCAAACTTTGCTATCGTCAGAGATTGTGGTCGCTTCCGT

GTTAGTGCTTTTTTTCAGCGAGAACTGCCGGGTGCAGTTATTCGCCGTATCGAAACCATCATTCCTACTTTTGAGCAGTT

AAAACTGCCTTTAGTGCTGCAAGACTTAGCGATTGCCAAGCGTGGTTTGGTGCTGGTGGTCGGCGCGACTGGCTCGGGTA

AATCGACCACGATGGCGGCGATGACGGGCTATCGAAACAGCAACAAAACCGGTCACATCTTGACGGTCGAAGATCCGATC

GAATTTGTGCATGAGCATAAGCGTTGTATCGTGACTCAGCGTGAAGTGGGTTTGGATACCGAAAGCTATGAAGTCGCACT

GAAAAACTCGCTGCGCCAAGCGCCAGATATGATTTTGATTGGTGAGATCCGTAGCCGAGAAACGATGGAATACGCGATGA

CCTTCGCCGAAACGGGTCACTTGTGTATGGCAACGTTGCACGCCAACAACGCCAACCAAGCGTTAGAACGTATTCTCCAT

TTGATGCCTAAAGATCAAAAAGAGCAATTCTTGTTTGATTTGTCGATGAACCTAAAAGGCGTGATTGGTCAGCAGCTGAT

CCGTGATAAGAATGGTCAAGGGCGTCATGGTGTGTTTGAGATCTTGCTAAACAGCCCACGAGTGTCGGATTTGATCCGTC

GTGGTGATTTACACGAATTGAAATCAACCATGGCACGATCCAATGAGTTTGGCATGCTGACGTTTGACCAATCGCTCTAC

AAGTTGGTGATGCAAGGTAAGATCAGCGAAGAGGATGCACTGCACAGTGCGGATTCTGCCAACGATCTTCGTTTAATGCT

GAAAACACAGCGTGGTGAGCCGTTCTCTACAGGCAGTCTTGCCAACGTCAAAATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-269)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilU	Vibrio cholerae strain A1552	82.609	100	0.826
pilU	Pseudomonas stutzeri DSM 10701	57.714	95.109	0.549
pilU	Acinetobacter baylyi ADP1	54.986	95.38	0.524
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.953	92.12	0.405
pilT	Acinetobacter nosocomialis M2	42.478	92.12	0.391
pilT	Acinetobacter baumannii D1279779	42.478	92.12	0.391
pilT	Acinetobacter baumannii strain A118	42.478	92.12	0.391
pilT	Legionella pneumophila strain Lp02	42.478	92.12	0.391
pilT	Legionella pneumophila strain ERS1305867	42.478	92.12	0.391
pilT	Pseudomonas aeruginosa PAK	42.183	92.12	0.389
pilT	Pseudomonas stutzeri DSM 10701	41.003	92.12	0.378
pilT	Acinetobacter baylyi ADP1	41.994	89.946	0.378
pilT	Vibrio cholerae strain A1552	41.018	90.761	0.372
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.018	90.761	0.372
pilT	Neisseria meningitidis 8013	40.361	90.217	0.364
pilT	Neisseria gonorrhoeae MS11	40.361	90.217	0.364

Detailed information NTG0702587