Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NIZ17_RS13350 Genome accession   NZ_CP099890
Coordinates   2681565..2682545 (+) Length   326 a.a.
NCBI ID   WP_256879848.1    Uniprot ID   -
Organism   Escherichia albertii strain 205_2_TBG_B     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2676565..2687545
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NIZ17_RS13320 (NIZ17_13310) - 2677170..2678177 (+) 1008 WP_000745183.1 DUF1202 family protein -
  NIZ17_RS13325 (NIZ17_13315) hemW 2678242..2679378 (-) 1137 WP_000239934.1 radical SAM family heme chaperone HemW -
  NIZ17_RS13330 (NIZ17_13320) - 2679371..2679964 (-) 594 WP_001174776.1 XTP/dITP diphosphatase -
  NIZ17_RS13335 (NIZ17_13325) yggU 2679972..2680262 (-) 291 WP_001277225.1 DUF167 family protein YggU -
  NIZ17_RS13340 (NIZ17_13330) yggT 2680259..2680825 (-) 567 WP_001094839.1 osmotic shock tolerance protein YggT -
  NIZ17_RS13345 (NIZ17_13335) yggS 2680843..2681547 (-) 705 WP_000997809.1 pyridoxal phosphate homeostasis protein -
  NIZ17_RS13350 (NIZ17_13340) pilT 2681565..2682545 (+) 981 WP_256879848.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NIZ17_RS13355 (NIZ17_13345) ruvX 2682729..2683145 (-) 417 WP_000017099.1 Holliday junction resolvase RuvX -
  NIZ17_RS13360 (NIZ17_13350) - 2683145..2683708 (-) 564 WP_002461296.1 YqgE/AlgH family protein -
  NIZ17_RS13365 (NIZ17_13355) gshB 2683817..2684767 (-) 951 WP_000593300.1 glutathione synthase -
  NIZ17_RS13370 (NIZ17_13360) rsmE 2684780..2685511 (-) 732 WP_002461297.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NIZ17_RS13375 (NIZ17_13365) endA 2685591..2686298 (-) 708 WP_002461298.1 deoxyribonuclease I -
  NIZ17_RS13380 (NIZ17_13370) - 2686393..2686890 (-) 498 WP_002461299.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35973.14 Da        Isoelectric Point: 5.7939

>NTDB_id=702253 NIZ17_RS13350 WP_256879848.1 2681565..2682545(+) (pilT) [Escherichia albertii strain 205_2_TBG_B]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDIEVLLREWLDDDQRALLLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLNNENGLILVTGATGSGKSTTLAAMVGYLNQHTDAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSEAIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=702253 NIZ17_RS13350 WP_256879848.1 2681565..2682545(+) (pilT) [Escherichia albertii strain 205_2_TBG_B]
ATGAATATGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGTAGCGCCTGGCCTGCACG
CTGGCGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATACGCCGGACATTGAAGTGCTGCTAAGGGAATGGCTGGATG
ACGATCAGCGAGCGTTATTGCTGGAGAATGGGCAGTTGGACTTTGCCGTGTCGTTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGTTGCTGCCTTCGCATTGCCCGCAACTCGAACAGCTTGG
TGCGCCGACGGTATTACCGGAACTACTCAATAACGAGAATGGACTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CCACCACACTGGCGGCGATGGTTGGTTATCTCAATCAGCATACCGATGCGCATATTCTTACGCTGGAAGATCCTGTGGAA
TATCTTTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTTGCATCGGGATTACG
TGCGGCATTGCGGGAAGATCCCGATGTAATTTTGCTTGGCGAGCTGCGTGACAGCGAGGCCATTCGTCTGGCGTTGACGG
CGGCAGAAACAGGTCATCTGGTGCTGGCAACCTTGCATACGCGCGGAGCGGCTCAGGCGGTCGAGCGGCTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCGGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

44.343

100

0.445
  pilT Legionella pneumophila strain Lp02

44.343

100

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

40.063

97.239

0.39
  pilU Pseudomonas stutzeri DSM 10701

37.952

100

0.387
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362