Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NDO72_RS03670 Genome accession   NZ_CP099834
Coordinates   738437..739417 (+) Length   326 a.a.
NCBI ID   WP_032454049.1    Uniprot ID   A0AAI8IVY5
Organism   Klebsiella quasipneumoniae strain Y7-3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 733437..744417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDO72_RS03640 (NDO72_03640) - 733819..734805 (-) 987 WP_023290997.1 TRAP transporter substrate-binding protein -
  NDO72_RS03645 (NDO72_03645) hemW 735110..736246 (-) 1137 WP_023319135.1 radical SAM family heme chaperone HemW -
  NDO72_RS03650 (NDO72_03650) - 736239..736832 (-) 594 WP_023290995.1 XTP/dITP diphosphatase -
  NDO72_RS03655 (NDO72_03655) yggU 736845..737135 (-) 291 WP_004205183.1 DUF167 family protein YggU -
  NDO72_RS03660 (NDO72_03660) - 737132..737698 (-) 567 WP_002916613.1 YggT family protein -
  NDO72_RS03665 (NDO72_03665) - 737719..738420 (-) 702 WP_004205181.1 YggS family pyridoxal phosphate-dependent enzyme -
  NDO72_RS03670 (NDO72_03670) pilT 738437..739417 (+) 981 WP_032454049.1 type IV pilus twitching motility protein PilT Machinery gene
  NDO72_RS03675 (NDO72_03675) - 739766..740419 (+) 654 WP_032454048.1 LuxR C-terminal-related transcriptional regulator -
  NDO72_RS03680 (NDO72_03680) ruvX 740471..740887 (-) 417 WP_004205178.1 Holliday junction resolvase RuvX -
  NDO72_RS03685 (NDO72_03685) - 740887..741450 (-) 564 WP_002916603.1 YqgE/AlgH family protein -
  NDO72_RS03690 (NDO72_03690) gshB 741625..742575 (-) 951 WP_004205176.1 glutathione synthase -
  NDO72_RS03695 (NDO72_03695) rsmE 742596..743327 (-) 732 WP_023290991.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NDO72_RS03700 (NDO72_03700) endA 743419..744126 (-) 708 WP_032454045.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35397.82 Da        Isoelectric Point: 6.8575

>NTDB_id=701852 NDO72_RS03670 WP_032454049.1 738437..739417(+) (pilT) [Klebsiella quasipneumoniae strain Y7-3]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGKLEAAPFPAPDIADLLNDWLDAAQFQHWQEHGQIDFALTLACGSRLRA
SAFAHTRGISLVLRLLPVQCPRLETLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREIGRHCPSFSAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQR
VATGQL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=701852 NDO72_RS03670 WP_032454049.1 738437..739417(+) (pilT) [Klebsiella quasipneumoniae strain Y7-3]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGCAAGCTGGAGGCCGCTCCCTTTCCCGCGCCGGATATCGCGGATTTACTCAACGACTGGCTTGATG
CCGCACAGTTCCAGCACTGGCAGGAGCACGGCCAGATTGACTTTGCGCTGACTCTGGCCTGCGGTTCGCGGCTACGCGCC
AGCGCTTTCGCCCATACGCGCGGAATATCGCTGGTGCTGCGTCTGCTGCCCGTCCAGTGCCCGCGTCTGGAGACGCTGGG
CGCGCCGCCTGCGCTAAGCGAGCTGCTGGCGGAAGAGAGTGGCTTGCTGCTGGTCACCGGGGCGACGGGCAGCGGCAAGT
CGACTACCCTGGCGGCGATGGTCGGGCATCTCAACCAGCATCTTGATGGCCATATTCTGACGCTGGAGGATCCGGTGGAG
TTTATTCATCACAGCGAGCGCTGCCTGATCCAGCAGCGGGAGATTGGCCGTCACTGTCCTTCGTTCTCCGCGGCGCTTCG
GGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTTGGGGAGTTACGCGACAGCGAAACCATCCGTCTGGCGTTGACGG
CTGCGGAAACCGGGCATCTGGTGATGGCGACATTACACACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCAGAGGAGAAAGATCAGGTTCGGAGTCAGCTGGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTATTACC
CGCACGTCAGGGCGGCAGAGTTGCGCTATACGAGCTTCTGGTCAATACGCCGGCGGTGGCGAATTTGATCCGCGAAGGAA
AAGTGCACCAGCTGCCCGGTGTAATGCAGACCGGGATGCAGGCCGGAATGCTGACATTTACCCAGAGTTTTCAGCAACGT
GTGGCCACAGGCCAGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.988

100

0.521
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.988

100

0.521
  pilT Acinetobacter baylyi ADP1

48.624

100

0.488
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter nosocomialis M2

47.706

100

0.479
  pilT Legionella pneumophila strain ERS1305867

47.84

99.387

0.475
  pilT Legionella pneumophila strain Lp02

47.84

99.387

0.475
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.479

97.239

0.433
  pilU Vibrio cholerae strain A1552

40.729

100

0.411
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

39.252

98.466

0.386