Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LNQ24_RS02850 Genome accession   NZ_CP099813
Coordinates   659531..660556 (+) Length   341 a.a.
NCBI ID   WP_248577624.1    Uniprot ID   -
Organism   Yersinia ruckeri strain NVI-492     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 654531..665556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LNQ24_RS02820 (LNQ24_002825) hemW 655202..656332 (-) 1131 WP_038240979.1 radical SAM family heme chaperone HemW -
  LNQ24_RS02825 (LNQ24_002830) - 656325..656918 (-) 594 WP_004719276.1 XTP/dITP diphosphatase -
  LNQ24_RS02830 (LNQ24_002835) yggU 657019..657309 (-) 291 WP_038240982.1 DUF167 family protein YggU -
  LNQ24_RS02835 (LNQ24_002840) - 657306..657860 (-) 555 WP_004719273.1 YggT family protein -
  LNQ24_RS02840 (LNQ24_002845) proC 657964..658785 (-) 822 WP_004719272.1 pyrroline-5-carboxylate reductase -
  LNQ24_RS02845 (LNQ24_002850) - 658798..659511 (-) 714 WP_004719269.1 YggS family pyridoxal phosphate-dependent enzyme -
  LNQ24_RS02850 (LNQ24_002855) pilT 659531..660556 (+) 1026 WP_248577624.1 type IV pilus twitching motility protein PilT Machinery gene
  LNQ24_RS02855 (LNQ24_002860) aguA 660819..661904 (-) 1086 WP_248577625.1 agmatine deiminase -
  LNQ24_RS02860 (LNQ24_002865) aguB 661907..662791 (-) 885 WP_004719263.1 N-carbamoylputrescine amidase -
  LNQ24_RS02865 (LNQ24_002870) ruvX 663210..663635 (-) 426 WP_071704575.1 Holliday junction resolvase RuvX -
  LNQ24_RS02870 (LNQ24_002875) - 663635..664198 (-) 564 WP_248577626.1 YqgE/AlgH family protein -
  LNQ24_RS02875 (LNQ24_002880) gshB 664313..665272 (-) 960 WP_004719260.1 glutathione synthase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37139.58 Da        Isoelectric Point: 6.7505

>NTDB_id=701808 LNQ24_RS02850 WP_248577624.1 659531..660556(+) (pilT) [Yersinia ruckeri strain NVI-492]
MDFDEWVAASVKHNASDLHLCTGHYPVLRIAGDLHIFKHLTQVTPMWMENLAQQLLNEVHSLELQRHGQVDFSFSSSCGS
RLRGHFFRQQCGLSAAFRLIASQCPTLVDLEVPEVVHQLLPCESGLILVTGATGSGKSTTLAGMIGSINQTGGRHIITLE
DPVEFIHHSEGCLIQQRELGGHIASFGAGLKAALRQDPDIILLGELRDRETIALALTAAETGHLVLGTLHTRTAAQAVDR
LVDVFPAEEKTSVRSQLANSLMAVIAQKLVRRRGGGQVAVFETMIQTAAVSHLIREGKIHQLPGIMQTGGAMGMQTFEQG
LQQRQHQGLLPLAEDISQIHH

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=701808 LNQ24_RS02850 WP_248577624.1 659531..660556(+) (pilT) [Yersinia ruckeri strain NVI-492]
ATGGATTTTGATGAATGGGTGGCGGCTAGTGTAAAGCATAACGCCTCTGATCTGCACCTGTGCACTGGCCATTACCCGGT
GCTGCGTATTGCTGGTGACCTGCATATTTTCAAGCATCTCACTCAGGTAACGCCGATGTGGATGGAAAATCTGGCTCAGC
AGCTATTAAATGAGGTTCATTCTCTGGAATTACAACGGCATGGACAGGTCGATTTTTCATTTTCATCATCTTGTGGATCA
AGATTGAGAGGGCATTTCTTTCGGCAGCAGTGCGGGCTTTCAGCCGCTTTTCGCTTGATTGCGTCCCAATGCCCAACATT
GGTTGATCTTGAAGTTCCGGAAGTTGTTCATCAATTATTGCCCTGTGAAAGTGGTTTGATTTTGGTAACGGGAGCCACGG
GTAGTGGGAAATCAACCACTCTGGCGGGGATGATTGGATCCATCAATCAAACGGGCGGGCGGCATATTATTACATTGGAA
GATCCGGTTGAGTTTATTCATCACAGTGAGGGTTGTCTGATTCAACAGCGGGAGTTGGGTGGACATATTGCTTCTTTTGG
CGCGGGGCTAAAAGCGGCACTAAGGCAGGATCCCGATATTATCTTGTTGGGAGAGCTACGAGATCGGGAAACTATTGCAC
TTGCGTTGACGGCGGCGGAAACCGGGCATTTGGTGTTGGGCACACTACATACCCGGACAGCCGCGCAGGCGGTGGATCGA
TTGGTTGATGTATTTCCAGCTGAAGAAAAAACCAGTGTGCGAAGCCAGCTAGCAAACAGCCTGATGGCGGTAATTGCGCA
AAAGCTGGTGCGGCGTCGCGGAGGAGGGCAGGTGGCGGTGTTTGAAACCATGATACAGACAGCGGCAGTTAGCCATTTGA
TACGCGAGGGAAAAATCCACCAACTACCTGGAATTATGCAAACCGGCGGTGCAATGGGAATGCAAACTTTTGAGCAGGGA
TTACAACAGCGTCAACATCAGGGGTTATTGCCATTGGCGGAAGACATTAGCCAGATACATCATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.558

99.413

0.493
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.558

99.413

0.493
  pilT Neisseria gonorrhoeae MS11

48.338

97.067

0.469
  pilT Neisseria meningitidis 8013

48.338

97.067

0.469
  pilT Pseudomonas stutzeri DSM 10701

48.182

96.774

0.466
  pilT Acinetobacter baylyi ADP1

46.471

99.707

0.463
  pilT Pseudomonas aeruginosa PAK

47.576

96.774

0.46
  pilT Legionella pneumophila strain ERS1305867

46.246

97.654

0.452
  pilT Legionella pneumophila strain Lp02

46.246

97.654

0.452
  pilT Acinetobacter nosocomialis M2

46.061

96.774

0.446
  pilT Acinetobacter baumannii strain A118

46.894

94.428

0.443
  pilT Acinetobacter baumannii D1279779

46.894

94.428

0.443
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.32

93.548

0.396
  pilU Pseudomonas stutzeri DSM 10701

39.21

96.481

0.378
  pilU Acinetobacter baylyi ADP1

37.952

97.361

0.37
  pilU Vibrio cholerae strain A1552

37.963

95.015

0.361