Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LOR96_RS14325 Genome accession   NZ_CP099805
Coordinates   3090660..3091685 (-) Length   341 a.a.
NCBI ID   WP_071667004.1    Uniprot ID   -
Organism   Yersinia ruckeri strain NVI-10705     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3085660..3096685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOR96_RS14300 (LOR96_014290) gshB 3085944..3086903 (+) 960 WP_071704576.1 glutathione synthase -
  LOR96_RS14305 (LOR96_014295) - 3087018..3087581 (+) 564 WP_038240991.1 YqgE/AlgH family protein -
  LOR96_RS14310 (LOR96_014300) ruvX 3087581..3088006 (+) 426 WP_038240988.1 Holliday junction resolvase RuvX -
  LOR96_RS14315 (LOR96_014305) aguB 3088425..3089309 (+) 885 WP_004719263.1 N-carbamoylputrescine amidase -
  LOR96_RS14320 (LOR96_014310) aguA 3089312..3090397 (+) 1086 WP_096823602.1 agmatine deiminase -
  LOR96_RS14325 (LOR96_014315) pilT 3090660..3091685 (-) 1026 WP_071667004.1 type IV pilus twitching motility protein PilT Machinery gene
  LOR96_RS14330 (LOR96_014320) - 3091705..3092418 (+) 714 WP_004719269.1 YggS family pyridoxal phosphate-dependent enzyme -
  LOR96_RS14335 (LOR96_014325) proC 3092431..3093252 (+) 822 WP_004719272.1 pyrroline-5-carboxylate reductase -
  LOR96_RS14340 (LOR96_014330) - 3093356..3093910 (+) 555 WP_004719273.1 YggT family protein -
  LOR96_RS14345 (LOR96_014335) yggU 3093907..3094197 (+) 291 WP_038240982.1 DUF167 family protein YggU -
  LOR96_RS14350 (LOR96_014340) - 3094298..3094891 (+) 594 WP_004719276.1 XTP/dITP diphosphatase -
  LOR96_RS14355 (LOR96_014345) hemW 3094884..3096014 (+) 1131 WP_038240979.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37104.48 Da        Isoelectric Point: 6.7173

>NTDB_id=701732 LOR96_RS14325 WP_071667004.1 3090660..3091685(-) (pilT) [Yersinia ruckeri strain NVI-10705]
MDFDEWVAASVKHNASDLHLCTGHYPVLRIAGDLHIFKHLTQVTPMWMENLAQQLLNEVHSLELQRNGQVDFSFSSSCGS
RLRGHFFRQQCGLSAAFRLIASQCPTLVDLEVPEVVHQLLPCESGLILVTGATGSGKSTTLAGMIGSINQTGGRHIITLE
DPVEFIHHSEGCLIQQRELGGHIASFGAGLKAALRQDPDIILLGELRDRETIALALTAAETGHLVLGTLHTRTAAQAVDR
LVDVFPAEEKTSVRSQLANSLMAVIAQKLVRRRGGGQVAVFETMIQTAAVSHLIREGKTHQLPGIMQTGGAMGMQTFEQG
LQQRQHQGLLPLAEDISQIHH

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=701732 LOR96_RS14325 WP_071667004.1 3090660..3091685(-) (pilT) [Yersinia ruckeri strain NVI-10705]
ATGGATTTTGATGAATGGGTGGCGGCTAGTGTAAAGCATAACGCCTCTGATCTGCACCTGTGCACTGGCCATTACCCGGT
GCTGCGTATTGCTGGTGACCTGCATATTTTCAAGCATCTCACTCAGGTAACGCCGATGTGGATGGAAAATCTGGCTCAGC
AGCTATTAAATGAGGTTCATTCTCTGGAATTACAACGGAATGGACAGGTCGATTTTTCATTTTCATCATCTTGTGGATCA
AGATTGAGAGGGCATTTCTTTCGGCAGCAGTGCGGGCTTTCAGCCGCTTTTCGCTTGATTGCGTCCCAATGCCCAACATT
GGTTGATCTTGAAGTTCCGGAAGTTGTTCATCAATTATTGCCCTGTGAAAGTGGTTTGATTTTGGTAACGGGAGCCACGG
GTAGTGGGAAATCAACCACTCTGGCGGGGATGATTGGATCCATCAATCAAACGGGCGGGCGGCATATTATTACATTGGAA
GATCCGGTTGAGTTTATTCATCACAGTGAGGGTTGTCTGATTCAACAGCGGGAGTTGGGTGGACATATTGCTTCTTTTGG
CGCGGGGCTAAAAGCGGCACTAAGGCAGGATCCCGATATTATCTTGTTGGGAGAGCTACGAGATCGGGAAACTATTGCAC
TTGCGTTGACGGCGGCGGAAACCGGGCATTTGGTGTTGGGCACACTACATACCCGGACAGCCGCGCAGGCGGTGGATCGA
TTGGTTGATGTATTTCCAGCTGAAGAAAAAACCAGTGTGCGAAGCCAGCTAGCAAACAGCCTGATGGCGGTAATTGCGCA
AAAGCTGGTGCGGCGTCGCGGAGGAGGGCAGGTGGCGGTGTTTGAAACCATGATACAGACAGCGGCAGTTAGCCATTTGA
TACGCGAGGGAAAAACCCACCAACTACCTGGAATTATGCAAACCGGCGGTGCAATGGGAATGCAAACTTTTGAGCAGGGA
TTACAACAGCGTCAACATCAGGGGTTATTGCCATTGGCGGAAGACATTAGCCAGATACATCATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.558

99.413

0.493
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.558

99.413

0.493
  pilT Neisseria meningitidis 8013

48.338

97.067

0.469
  pilT Neisseria gonorrhoeae MS11

48.338

97.067

0.469
  pilT Pseudomonas stutzeri DSM 10701

48.182

96.774

0.466
  pilT Acinetobacter baylyi ADP1

46.471

99.707

0.463
  pilT Pseudomonas aeruginosa PAK

47.576

96.774

0.46
  pilT Legionella pneumophila strain ERS1305867

45.946

97.654

0.449
  pilT Legionella pneumophila strain Lp02

45.946

97.654

0.449
  pilT Acinetobacter nosocomialis M2

46.061

96.774

0.446
  pilT Acinetobacter baumannii strain A118

46.894

94.428

0.443
  pilT Acinetobacter baumannii D1279779

46.894

94.428

0.443
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.633

93.548

0.399
  pilU Pseudomonas stutzeri DSM 10701

39.514

96.481

0.381
  pilU Acinetobacter baylyi ADP1

37.651

97.361

0.367
  pilU Vibrio cholerae strain A1552

37.963

95.015

0.361