Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NH397_RS09400 Genome accession   NZ_CP099799
Coordinates   1920269..1921324 (-) Length   351 a.a.
NCBI ID   WP_066674448.1    Uniprot ID   -
Organism   Clostridium septicum strain RMA 8861     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1915269..1926324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH397_RS09380 (NH397_09380) sigG 1915622..1916395 (-) 774 WP_066674470.1 RNA polymerase sporulation sigma factor SigG -
  NH397_RS09385 (NH397_09385) sigE 1916477..1917184 (-) 708 WP_066674467.1 RNA polymerase sporulation sigma factor SigE -
  NH397_RS09390 (NH397_09390) - 1917194..1917994 (-) 801 WP_066674455.1 sigma-E processing peptidase SpoIIGA -
  NH397_RS09395 (NH397_09395) ftsZ 1918215..1919339 (-) 1125 WP_066674451.1 cell division protein FtsZ -
  NH397_RS09400 (NH397_09400) pilT 1920269..1921324 (-) 1056 WP_066674448.1 type IV pilus twitching motility protein PilT Machinery gene
  NH397_RS09405 (NH397_09405) sigK 1921430..1922125 (-) 696 WP_066674446.1 RNA polymerase sporulation sigma factor SigK -
  NH397_RS09410 (NH397_09410) - 1922419..1924032 (-) 1614 WP_066674443.1 penicillin-binding transpeptidase domain-containing protein -
  NH397_RS09415 (NH397_09415) udk 1924090..1924707 (-) 618 WP_066674439.1 uridine kinase -
  NH397_RS09420 (NH397_09420) - 1924729..1925964 (-) 1236 WP_066674438.1 U32 family peptidase -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38973.18 Da        Isoelectric Point: 8.6871

>NTDB_id=701669 NH397_RS09400 WP_066674448.1 1920269..1921324(-) (pilT) [Clostridium septicum strain RMA 8861]
MYILRDLLSKTIEAEASDLHLTVGVPPIIRNNGRLIKLGTNKLTPEDIEAFAKEILQAKYEEYNKIGELDISYSVPGVGR
FRVNVFKQRNSDAIAIRVIALKIPTLDELKHPAVIKELVQKQRGLVLVTGPAGSGKSTTLAAMINEINSTREAHIITLED
PIEYLHKHNKCIINQREIGKDSSSYKSALKAILREDPDVILVGEMRDLETISIALTAAETGHLVFSTLHTIGAAKTIDRI
IDVFPPHQQQQVRVQLSAMLKGVISQQLVPNADNTGRVVALETMITTPGIQNLIREGKTYQIESAIQTGSKYGMKTMDMS
LAELYRRGTISIETAFSHSVDRETIKRIMML

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=701669 NH397_RS09400 WP_066674448.1 1920269..1921324(-) (pilT) [Clostridium septicum strain RMA 8861]
ATGTATATTTTACGTGATTTATTAAGTAAGACAATAGAGGCAGAAGCATCAGATCTACATTTGACAGTTGGGGTACCGCC
AATTATTAGGAATAATGGAAGATTGATAAAGCTAGGAACTAACAAACTAACACCAGAAGATATTGAAGCTTTTGCAAAAG
AAATATTACAAGCCAAATATGAGGAATATAATAAAATAGGTGAATTAGATATATCTTATTCTGTACCAGGAGTTGGCAGA
TTTAGAGTAAATGTATTTAAACAACGAAATAGTGATGCTATTGCAATAAGAGTTATTGCGTTAAAGATTCCTACTCTTGA
TGAACTTAAGCATCCTGCTGTAATAAAAGAATTAGTTCAAAAGCAGAGGGGATTAGTTCTAGTTACAGGACCAGCAGGTA
GTGGTAAAAGCACTACTCTTGCAGCTATGATAAATGAAATAAATAGTACAAGGGAGGCTCATATAATTACGTTAGAAGAT
CCAATTGAATATTTACACAAGCATAATAAGTGTATAATAAATCAAAGAGAAATTGGAAAAGATAGTAGTAGTTATAAAAG
TGCTTTAAAGGCTATATTAAGAGAAGATCCAGATGTTATTCTTGTAGGAGAAATGAGAGATTTAGAAACTATCTCAATTG
CACTAACAGCAGCTGAAACAGGTCATTTAGTATTTTCTACACTACATACAATAGGTGCTGCAAAGACTATAGATAGAATT
ATAGATGTTTTTCCACCACATCAACAGCAACAAGTTAGAGTTCAATTATCAGCTATGCTTAAAGGTGTGATTTCACAACA
ACTTGTTCCTAATGCAGATAATACAGGAAGGGTAGTAGCATTAGAAACAATGATAACAACTCCAGGAATACAAAATTTAA
TTAGAGAAGGAAAAACTTATCAAATAGAATCAGCAATACAAACTGGAAGCAAATATGGAATGAAGACAATGGATATGTCA
TTAGCTGAACTATATAGAAGGGGAACAATAAGTATAGAAACAGCTTTTAGCCATTCAGTTGATAGAGAAACTATTAAAAG
AATAATGATGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(85-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

52.454

92.877

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.454

92.877

0.487
  pilT Acinetobacter baumannii D1279779

51.36

94.302

0.484
  pilT Acinetobacter baumannii strain A118

51.36

94.302

0.484
  pilT Pseudomonas aeruginosa PAK

51.36

94.302

0.484
  pilT Acinetobacter nosocomialis M2

51.36

94.302

0.484
  pilT Acinetobacter baylyi ADP1

50.755

94.302

0.479
  pilT Neisseria gonorrhoeae MS11

48.974

97.151

0.476
  pilT Neisseria meningitidis 8013

48.974

97.151

0.476
  pilT Legionella pneumophila strain Lp02

48.521

96.296

0.467
  pilT Legionella pneumophila strain ERS1305867

48.521

96.296

0.467
  pilT Pseudomonas stutzeri DSM 10701

49.547

94.302

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.512

98.006

0.456
  pilU Pseudomonas stutzeri DSM 10701

41.739

98.291

0.41
  pilU Vibrio cholerae strain A1552

41.092

99.145

0.407
  pilU Acinetobacter baylyi ADP1

41.716

96.296

0.402