Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NF673_RS24855 Genome accession   NZ_CP099609
Coordinates   5596045..5597079 (-) Length   344 a.a.
NCBI ID   WP_016772732.1    Uniprot ID   V8R079
Organism   Pseudomonas moraviensis strain OTU5MASSA1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5591045..5602079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF673_RS24825 (NF673_24825) ruvX 5591215..5591652 (+) 438 WP_016772738.1 Holliday junction resolvase RuvX -
  NF673_RS24830 (NF673_24830) pyrR 5591734..5592240 (+) 507 WP_064364170.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  NF673_RS24835 (NF673_24835) - 5592268..5593272 (+) 1005 WP_252880927.1 aspartate carbamoyltransferase catalytic subunit -
  NF673_RS24840 (NF673_24840) - 5593269..5594540 (+) 1272 WP_042606567.1 dihydroorotase -
  NF673_RS24845 (NF673_24845) - 5594624..5595058 (-) 435 WP_252880928.1 TM2 domain-containing protein -
  NF673_RS24850 (NF673_24850) - 5595307..5595924 (+) 618 WP_042606566.1 C40 family peptidase -
  NF673_RS24855 (NF673_24855) pilT 5596045..5597079 (-) 1035 WP_016772732.1 type IV pilus twitching motility protein PilT Machinery gene
  NF673_RS24860 (NF673_24860) - 5597136..5597822 (+) 687 WP_064364755.1 YggS family pyridoxal phosphate-dependent enzyme -
  NF673_RS24865 (NF673_24865) proC 5597846..5598664 (+) 819 WP_064364753.1 pyrroline-5-carboxylate reductase -
  NF673_RS24870 (NF673_24870) - 5598676..5599266 (+) 591 WP_064364751.1 YggT family protein -
  NF673_RS24875 (NF673_24875) - 5599520..5600659 (+) 1140 WP_042606562.1 homoserine O-acetyltransferase -
  NF673_RS24880 (NF673_24880) metW 5600667..5601287 (+) 621 WP_024014659.1 methionine biosynthesis protein MetW -
  NF673_RS24885 (NF673_24885) - 5601314..5601748 (+) 435 WP_065615073.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38048.70 Da        Isoelectric Point: 6.9777

>NTDB_id=701089 NF673_RS24855 WP_016772732.1 5596045..5597079(-) (pilT) [Pseudomonas moraviensis strain OTU5MASSA1]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHDLIYDIMNDTQRIEFEKNLETDFSFDVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=701089 NF673_RS24855 WP_016772732.1 5596045..5597079(-) (pilT) [Pseudomonas moraviensis strain OTU5MASSA1]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGCGCATCCGACCTGCACCTGTCGGCGGGCCTGCCGCCGAT
GATCCGCGTCGATGGCGATGTGCGACGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGATCTGATCTACGACA
TCATGAACGACACCCAGCGCATCGAATTCGAGAAAAATCTGGAAACGGATTTTTCCTTTGATGTGCCCGGCGTGGCGCGG
TTTCGGGTCAACGCGTTCAACCAGAACCGAGGCGCCGGCGCAGTGTTCCGCACCATTCCCTCGAAAGTGCTGAGCATGGA
AGACCTCGCCATGGGCGATGTTTTCCGCAAGATCACCGATGCGCCGCGCGGGCTGGTGCTCGTCACCGGGCCGACCGGTT
CAGGCAAGTCCACCACGCTGGCGGCAATGATCGATTACCTCAACACCCATCGCCATCACCACATTCTCACCATTGAGGAC
CCCATCGAGTTTGTCCACGAATCGCGCAAATGCCTGATCAATCAGCGCGAAGTCCATCGCGATACCCGCAGCTTCGCCAC
AGCGCTGCGTTCGGCCCTGCGTGAAGATCCGGACGTGATTCTGGTGGGTGAGATGCGTGATCTGGAAACCATTCGCCTGG
CGCTGACCGCCGCTGAGACCGGGCATCTGGTGTTCGGCACACTGCACACGACCTCGGCGGCGAAGACCATCGACCGCGTG
GTGGACGTGTTCCCCGGCGACGAGAAGTCGATGGTGCGTTCGATGCTGTCGGAGTCGTTGCTGGCGGTGGTGTCGCAGAC
GTTGATCAAGAAGATCGGCGGCGGGCGGGTGGCGGCGCATGAAATCATGCTCGGGACATCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCAGCGATTCAGACCGGTGGGTCGTTGGGGATGCAGACACTCGATATGTGCCTG
AAGGATCTGGTGACCAAGGGCCTGATCAGCCGCGAGCATGCCCGCGAGAAAGCGCGCACCCCGGATAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V8R079

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.209

100

0.872
  pilT Pseudomonas stutzeri DSM 10701

87.209

100

0.872
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

79.07

100

0.791
  pilT Acinetobacter baumannii strain A118

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

77.035

100

0.77
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.751

100

0.41
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Vibrio cholerae strain A1552

40

100

0.401