Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NF673_RS02000 Genome accession   NZ_CP099609
Coordinates   453348..454412 (+) Length   354 a.a.
NCBI ID   WP_042608693.1    Uniprot ID   -
Organism   Pseudomonas moraviensis strain OTU5MASSA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 448348..459412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF673_RS01990 (NF673_01990) - 449058..450326 (+) 1269 WP_064363273.1 malic enzyme-like NAD(P)-binding protein -
  NF673_RS01995 (NF673_01995) - 450684..453131 (-) 2448 WP_042608692.1 penicillin-binding protein 1A -
  NF673_RS02000 (NF673_02000) pilM 453348..454412 (+) 1065 WP_042608693.1 pilus assembly protein PilM Machinery gene
  NF673_RS02005 (NF673_02005) - 454412..454978 (+) 567 WP_042608694.1 PilN domain-containing protein -
  NF673_RS02010 (NF673_02010) pilO 454975..455598 (+) 624 WP_042608695.1 type 4a pilus biogenesis protein PilO -
  NF673_RS02015 (NF673_02015) - 455595..456119 (+) 525 WP_039756678.1 pilus assembly protein PilP -
  NF673_RS02020 (NF673_02020) pilQ 456133..458211 (+) 2079 WP_252881172.1 type IV pilus secretin PilQ Machinery gene
  NF673_RS02025 (NF673_02025) aroK 458216..458734 (+) 519 WP_027611316.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38062.62 Da        Isoelectric Point: 4.8225

>NTDB_id=701066 NF673_RS02000 WP_042608693.1 453348..454412(+) (pilM) [Pseudomonas moraviensis strain OTU5MASSA1]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRLLVKARTGCKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATHLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVTEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLNTPTQVANP
FADMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=701066 NF673_RS02000 WP_042608693.1 453348..454412(+) (pilM) [Pseudomonas moraviensis strain OTU5MASSA1]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAACT
GAGTCGTCAGGGCGAGCGCTACCGGGTCGAGGCCTACGCGGTAGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAGAACA
TCGCCGAACTGGAGGGTGTCGGCCAGGCGCTCAGCCGGTTGCTGGTCAAGGCACGCACCGGGTGCAAGAGCGTGGCGGTG
GCGGTAGCCGGTTCGGCGGTGATCACCAAGGTCATCGAAATGGACGCCGGGCTGTCCGACGATGAACTGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTATCCGCTGGACGAGGTCGCCATCGACTTCGAAGTCCAGGGCGTCTCGCCGC
GCAACCCGGAACGGGTCAACGTGCTGCTCGCCGCCTGTCGCAAGGAAAACGTCGAGGTGCGCGAGGCGGCGCTGGCGCTC
GCCGGCCTGACTGCACGGGTGGTGGATGTCGAGGCCTACGCGCTGGAGCGCTCGTTCGGGCTGCTGGCGACGCATCTGGC
TGCTTCCCAGGAGCGCCTGACCGTGGCGGTGGTCGACATCGGCGCGACGATGACCACCCTCAGCGTCCTGCATAACGGCA
AGATCATCTATACCCGCGAGCAACTCTTTGGCGGGCGCCAGCTCACCGAAGAGATCCAGCGCCGCTATGGCCTCACTGTC
GAGCAGGCGGGCCTGGCGAAAAAGCAGGGCGGCCTGCCTGACGATTACGTGACCGAAGTCCTGCAGCCATTTCGCGAGGC
ACTGGTGCAGCAGGTTTCGCGTTCGCTGCAGTTCTTCTTCGCCTCTGGCCAGTACAACGCCGTCGACCATATCCTGCTGG
CCGGCGGCACGGCCTCTGTGCCCGGGCTGGATCGCCTGATCGAACAGCGCTTGAACACGCCGACCCAGGTTGCCAACCCG
TTTGCCGACATGGCCCTGGGCAGCAAGGTCAACGCCGGTGCGCTGGCCAGTGATGCGCCCGCATTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.023

100

0.48