Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   NF678_RS22020 Genome accession   NZ_CP099600
Coordinates   4983478..4983900 (-) Length   140 a.a.
NCBI ID   WP_252876912.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain OTU6MEDAA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4978478..4988900
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF678_RS22005 (NF678_22005) - 4979317..4982268 (+) 2952 WP_150742241.1 DUF748 domain-containing protein -
  NF678_RS22010 (NF678_22010) - 4982265..4982564 (+) 300 WP_150742242.1 DUF2845 domain-containing protein -
  NF678_RS22015 (NF678_22015) - 4982829..4983182 (-) 354 WP_071173629.1 BON domain-containing protein -
  NF678_RS22020 (NF678_22020) pilA/pilAI 4983478..4983900 (-) 423 WP_252876912.1 pilin Machinery gene
  NF678_RS22025 (NF678_22025) pilB 4984127..4985827 (+) 1701 WP_056790439.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NF678_RS22030 (NF678_22030) pilC 4985830..4987047 (+) 1218 WP_252876913.1 type II secretion system F family protein Machinery gene
  NF678_RS22035 (NF678_22035) pilD 4987049..4987921 (+) 873 WP_252878912.1 A24 family peptidase Machinery gene
  NF678_RS22040 (NF678_22040) coaE 4987918..4988541 (+) 624 WP_252882697.1 dephospho-CoA kinase -
  NF678_RS22045 (NF678_22045) yacG 4988538..4988738 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14087.26 Da        Isoelectric Point: 8.4918

>NTDB_id=700962 NF678_RS22020 WP_252876912.1 4983478..4983900(-) (pilA/pilAI) [Pseudomonas siliginis strain OTU6MEDAA1]
MKKQQGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISAMKVPFEDLINQGTNPSLAAVKGDNVTTTSNCA
LTASGTAADGAGTIVCTLANAPAPVLGKTITLTRTAAGGWSCGTTVAPDYSPKGCAASGT

Nucleotide


Download         Length: 423 bp        

>NTDB_id=700962 NF678_RS22020 WP_252876912.1 4983478..4983900(-) (pilA/pilAI) [Pseudomonas siliginis strain OTU6MEDAA1]
ATGAAAAAACAACAAGGTTTCACATTGATTGAGCTGCTGATCGTCGTGGCGATCATCGGCATTCTGGCGACGATCGCTTT
GCCGCAGTATTCGAAGTATCAGGCGCGGTCGAAGGTGACGGCGGGGCTGGCGGAGATCAGCGCGATGAAGGTGCCGTTTG
AAGACCTGATCAATCAGGGGACAAATCCAAGCTTGGCGGCGGTGAAAGGCGATAACGTCACCACTACCTCCAATTGCGCT
CTGACTGCCTCAGGTACCGCTGCAGACGGAGCTGGAACGATTGTATGTACGTTGGCCAACGCGCCAGCGCCTGTATTGGG
CAAAACCATCACGCTGACTCGTACGGCGGCCGGCGGCTGGAGTTGTGGCACTACGGTCGCTCCTGACTATTCGCCAAAAG
GCTGCGCTGCCAGTGGGACTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-135)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

50.365

97.857

0.493
  pilA Acinetobacter baumannii strain A118

46.763

99.286

0.464
  pilA Vibrio cholerae strain A1552

41.29

100

0.457
  pilA Vibrio cholerae C6706

41.29

100

0.457
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.29

100

0.457
  comP Acinetobacter baylyi ADP1

41.722

100

0.45
  pilA Pseudomonas aeruginosa PAK

41.06

100

0.443
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

96.429

0.429
  pilA Vibrio parahaemolyticus RIMD 2210633

42.222

96.429

0.407
  pilA2 Legionella pneumophila str. Paris

39.31

100

0.407
  pilA2 Legionella pneumophila strain ERS1305867

37.241

100

0.386
  pilA/pilA1 Eikenella corrodens VA1

39.259

96.429

0.379