Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NF676_RS25470 Genome accession   NZ_CP099598
Coordinates   5540394..5541428 (-) Length   344 a.a.
NCBI ID   WP_016772732.1    Uniprot ID   V8R079
Organism   Pseudomonas siliginis strain OTU6BANIB1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5535394..5546428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF676_RS25440 (NF676_25445) ruvX 5535522..5535959 (+) 438 WP_252886929.1 Holliday junction resolvase RuvX -
  NF676_RS25445 (NF676_25450) pyrR 5536041..5536547 (+) 507 WP_016772737.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  NF676_RS25450 (NF676_25455) - 5536575..5537579 (+) 1005 WP_016772736.1 aspartate carbamoyltransferase catalytic subunit -
  NF676_RS25455 (NF676_25460) - 5537576..5538847 (+) 1272 WP_150743563.1 dihydroorotase -
  NF676_RS25460 (NF676_25465) - 5538943..5539377 (-) 435 WP_016772734.1 TM2 domain-containing protein -
  NF676_RS25465 (NF676_25470) - 5539626..5540243 (+) 618 WP_024014657.1 C40 family peptidase -
  NF676_RS25470 (NF676_25475) pilT 5540394..5541428 (-) 1035 WP_016772732.1 type IV pilus twitching motility protein PilT Machinery gene
  NF676_RS25475 (NF676_25480) - 5541485..5542171 (+) 687 WP_071172225.1 YggS family pyridoxal phosphate-dependent enzyme -
  NF676_RS25480 (NF676_25485) proC 5542195..5543013 (+) 819 WP_041476815.1 pyrroline-5-carboxylate reductase -
  NF676_RS25485 (NF676_25490) - 5543025..5543615 (+) 591 WP_039757546.1 YggT family protein -
  NF676_RS25490 (NF676_25495) - 5543821..5544960 (+) 1140 WP_083353760.1 homoserine O-acetyltransferase -
  NF676_RS25495 (NF676_25500) metW 5544968..5545588 (+) 621 WP_056786380.1 methionine biosynthesis protein MetW -
  NF676_RS25500 (NF676_25505) - 5545615..5546049 (+) 435 WP_252886930.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38048.70 Da        Isoelectric Point: 6.9777

>NTDB_id=700913 NF676_RS25470 WP_016772732.1 5540394..5541428(-) (pilT) [Pseudomonas siliginis strain OTU6BANIB1]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHDLIYDIMNDTQRIEFEKNLETDFSFDVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=700913 NF676_RS25470 WP_016772732.1 5540394..5541428(-) (pilT) [Pseudomonas siliginis strain OTU6BANIB1]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGCGCTTCCGACCTGCACCTGTCGGCCGGCCTGCCGCCGAT
GATCCGTGTCGATGGCGACGTGCGACGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGATCTGATCTACGACA
TCATGAACGACACCCAGCGCATCGAATTCGAGAAAAATCTGGAAACGGATTTTTCCTTCGATGTACCCGGCGTGGCGCGC
TTTCGGGTCAACGCATTCAACCAGAACCGTGGCGCCGGCGCCGTGTTCCGCACTATTCCGTCGAAAGTGCTGAGCATGGA
AGACCTCGCCATGGGCGATGTTTTCCGCAAGATCACGGACGCGCCGCGCGGGCTGGTGCTGGTCACCGGGCCGACCGGCT
CCGGCAAATCGACCACGCTGGCGGCGATGATCGATTACCTCAACACCCATCGCCATCACCACATCCTCACCATTGAGGAC
CCCATCGAGTTCGTCCATGAATCGCGCAAATGCCTGATCAATCAGCGCGAAGTCCATCGCGATACGCGCAGCTTCGCCAC
CGCACTGCGTTCGGCCTTGCGTGAAGACCCGGACGTGATTCTGGTCGGCGAAATGCGTGATCTGGAAACCATTCGCCTGG
CGCTGACCGCCGCCGAGACCGGGCATCTGGTGTTCGGCACCCTGCACACGACCTCGGCGGCGAAGACCATCGACCGCGTG
GTCGACGTGTTCCCCGGCGACGAGAAGTCGATGGTCCGTTCGATGCTGTCGGAGTCGTTGCTGGCGGTGGTGTCGCAGAC
TTTGATCAAGAAGATCGGCGGCGGGCGGGTGGCGGCGCATGAAATCATGCTCGGGACATCGGCGATTCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATTCAGACCGGTGGGTCGTTGGGGATGCAGACACTCGATATGTGCCTG
AAGGATCTGGTGACCAAGGGCCTGATCAGCCGCGAGCATGCGCGGGAGAAGGCGCGTACGCCTGATAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V8R079

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.209

100

0.872
  pilT Pseudomonas stutzeri DSM 10701

87.209

100

0.872
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

79.07

100

0.791
  pilT Acinetobacter baumannii strain A118

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

77.035

100

0.77
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.751

100

0.41
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Vibrio cholerae strain A1552

40

100

0.401