Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MN084_RS02325 Genome accession   NZ_CP099567
Coordinates   413324..414361 (+) Length   345 a.a.
NCBI ID   WP_241084996.1    Uniprot ID   -
Organism   Candidatus Vondammii sp. HM_W22     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 408324..419361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MN084_RS02295 - 409305..409598 (+) 294 WP_241085001.1 transposase -
  MN084_RS02300 - 410013..410312 (-) 300 WP_241085000.1 DUF167 family protein -
  MN084_RS02305 - 410332..410913 (-) 582 WP_241084999.1 YggT family protein -
  MN084_RS02310 proC 410913..411773 (-) 861 WP_320416328.1 pyrroline-5-carboxylate reductase -
  MN084_RS02315 - 411807..412490 (-) 684 WP_241084998.1 YggS family pyridoxal phosphate-dependent enzyme -
  MN084_RS02320 - 412738..413232 (+) 495 WP_241084997.1 transposase -
  MN084_RS02325 pilT 413324..414361 (+) 1038 WP_241084996.1 type IV pilus twitching motility protein PilT Machinery gene
  MN084_RS02330 - 414441..415572 (+) 1132 Protein_454 PilT/PilU family type 4a pilus ATPase -
  MN084_RS02335 - 415594..416472 (-) 879 WP_241084995.1 dihydroorotate dehydrogenase electron transfer subunit -
  MN084_RS02340 - 416684..416860 (-) 177 WP_241084994.1 hypothetical protein -
  MN084_RS02345 - 416874..417798 (-) 925 Protein_457 IS3 family transposase -
  MN084_RS02350 - 418014..418208 (+) 195 WP_241084993.1 Mor transcription activator family protein -
  MN084_RS02355 - 418394..418678 (-) 285 WP_330178513.1 transposase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38207.97 Da        Isoelectric Point: 6.7275

>NTDB_id=700696 MN084_RS02325 WP_241084996.1 413324..414361(+) (pilT) [Candidatus Vondammii sp. HM_W22]
MDIAELLAFSVKHNASDLHLSAGLPPMIRVDGDIRRVNVLALEHKDVHALVYDIMNDRQRKDYEEFLEADFSFEIRGLAR
FRVNAFNQDRGASAVFRTIPSKVLTLEELGCPEGFKEIVDVPRGLVLVTGPTGSGKSTTLAAMVDYKNDNDYAHILTIED
PIEFVHTSKKCLISQREVHRDTLGFAEALRSALREDPDVILVGEMRDLETIRLAMTAAETGHVVFGTLHTSSAAKTIDRI
IDAFPAAEKSMIRSMLAESLRAVIAQTLLKKVGGGRTAAWEVMVGTPAIRNLIREDKVAQMYSAIQTGQAHGMQTLDQHL
QDLVKRGMISRLDARSKAMNKGIFV

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=700696 MN084_RS02325 WP_241084996.1 413324..414361(+) (pilT) [Candidatus Vondammii sp. HM_W22]
ATGGATATTGCTGAACTTCTCGCATTCAGCGTTAAGCACAATGCTTCGGATCTACACCTTTCGGCAGGCTTGCCGCCAAT
GATCCGCGTGGATGGTGATATACGCCGGGTCAATGTGCTGGCGCTTGAACATAAAGATGTGCATGCGCTGGTCTACGATA
TTATGAATGACAGGCAGCGCAAGGATTACGAGGAGTTCCTCGAGGCCGACTTCTCATTCGAAATTCGCGGCCTTGCGCGT
TTCCGTGTTAATGCCTTCAATCAGGATCGTGGTGCATCGGCGGTGTTCCGGACCATTCCCTCCAAGGTACTGACGCTGGA
GGAGTTAGGCTGCCCTGAGGGGTTCAAAGAGATTGTCGATGTTCCTAGAGGGCTGGTTCTGGTAACCGGGCCTACCGGCT
CTGGTAAGTCCACCACTCTTGCGGCCATGGTGGATTACAAGAACGATAATGACTACGCTCACATTCTGACGATTGAAGAT
CCTATCGAATTCGTGCATACCAGTAAGAAATGTCTGATCAGTCAGCGCGAAGTACACCGGGATACTCTAGGTTTTGCCGA
GGCACTTCGTTCTGCACTCCGCGAAGACCCGGATGTTATTCTGGTAGGTGAAATGCGCGATCTGGAAACTATCCGGCTGG
CAATGACTGCAGCAGAAACGGGGCATGTTGTTTTTGGTACCCTGCATACCAGTTCCGCAGCGAAAACCATCGACCGTATC
ATTGACGCTTTCCCTGCAGCGGAAAAATCGATGATCCGTTCCATGCTGGCGGAGTCGCTACGTGCCGTTATTGCCCAGAC
GTTGCTGAAAAAGGTAGGTGGCGGCAGAACGGCAGCCTGGGAGGTCATGGTGGGTACCCCGGCTATCCGCAATCTGATCC
GTGAAGACAAGGTGGCACAGATGTATTCCGCGATCCAGACAGGGCAGGCTCACGGAATGCAGACTCTTGATCAGCACCTG
CAGGATCTGGTGAAGAGGGGTATGATAAGCCGTCTGGATGCTCGTAGCAAAGCGATGAACAAAGGGATATTTGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

79.586

97.971

0.78
  pilT Pseudomonas stutzeri DSM 10701

78.994

97.971

0.774
  pilT Acinetobacter baumannii D1279779

77.101

100

0.771
  pilT Acinetobacter baumannii strain A118

77.101

100

0.771
  pilT Acinetobacter nosocomialis M2

77.101

100

0.771
  pilT Acinetobacter baylyi ADP1

74.709

99.71

0.745
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.92

97.681

0.693
  pilT Vibrio cholerae strain A1552

70.92

97.681

0.693
  pilT Neisseria meningitidis 8013

65.396

98.841

0.646
  pilT Neisseria gonorrhoeae MS11

65.103

98.841

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.964

95.942

0.499
  pilU Vibrio cholerae strain A1552

42.985

97.101

0.417
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.101

0.406
  pilU Acinetobacter baylyi ADP1

40

98.551

0.394