Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NDO58_RS22650 Genome accession   NZ_CP099513
Coordinates   4829305..4830285 (-) Length   326 a.a.
NCBI ID   WP_004135845.1    Uniprot ID   A0A285B9N8
Organism   Klebsiella sp. ZYC-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4824305..4835285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDO58_RS22620 (NDO58_22590) endA 4824594..4825301 (+) 708 WP_024358646.1 deoxyribonuclease I -
  NDO58_RS22625 (NDO58_22595) rsmE 4825392..4826123 (+) 732 WP_024358647.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NDO58_RS22630 (NDO58_22600) gshB 4826144..4827091 (+) 948 WP_004135852.1 glutathione synthase -
  NDO58_RS22635 (NDO58_22605) - 4827268..4827831 (+) 564 WP_004135851.1 YqgE/AlgH family protein -
  NDO58_RS22640 (NDO58_22610) ruvX 4827831..4828247 (+) 417 WP_004135849.1 Holliday junction resolvase RuvX -
  NDO58_RS22645 (NDO58_22615) - 4828303..4828956 (-) 654 WP_004135847.1 LuxR C-terminal-related transcriptional regulator -
  NDO58_RS22650 (NDO58_22620) pilT 4829305..4830285 (-) 981 WP_004135845.1 type IV pilus twitching motility protein PilT Machinery gene
  NDO58_RS22655 (NDO58_22625) - 4830302..4831015 (+) 714 WP_004125172.1 YggS family pyridoxal phosphate-dependent enzyme -
  NDO58_RS22660 (NDO58_22630) - 4831024..4831590 (+) 567 WP_004135843.1 YggT family protein -
  NDO58_RS22665 (NDO58_22635) yggU 4831587..4831877 (+) 291 WP_004125173.1 DUF167 family protein YggU -
  NDO58_RS22670 (NDO58_22640) - 4831890..4832483 (+) 594 WP_024358651.1 XTP/dITP diphosphatase -
  NDO58_RS22675 (NDO58_22645) hemW 4832476..4833612 (+) 1137 WP_064343199.1 radical SAM family heme chaperone HemW -
  NDO58_RS22680 (NDO58_22650) - 4833917..4834903 (+) 987 WP_049099832.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35153.28 Da        Isoelectric Point: 6.5804

>NTDB_id=700344 NDO58_RS22650 WP_004135845.1 4829305..4830285(-) (pilT) [Klebsiella sp. ZYC-1]
MELEEIVALSVKHNVSDLHLCNASAPRWRRQGKLEPAPFTSPDIGKILTDWLNAEQLAQWQACGQVDFALTPADGPRLRA
SAFAHNRGLSLALRLLAETCPQLADLGAPAALTELLNEESGLILVTGATGSGKSTTLAAMVGYLNQRLSGHILTLEDPVE
FIHRSERCLIQQREIGRHCPSFAAALRAALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAQAVERLVDV
FPAQEKEQVRSQLAGSLCAVLAQKLFPDAAGGRVALYELLVNTPAVANLIREGKTHQLPGVMQTGQQSGMQTFTQSFQQR
TAAGLL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=700344 NDO58_RS22650 WP_004135845.1 4829305..4830285(-) (pilT) [Klebsiella sp. ZYC-1]
ATGGAACTGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAATGCTTCAGCGCCGCG
CTGGCGTCGCCAGGGCAAGCTGGAGCCCGCGCCGTTTACCTCTCCCGATATTGGCAAGATACTCACTGATTGGCTCAACG
CCGAGCAGCTCGCGCAGTGGCAGGCCTGCGGGCAGGTCGATTTCGCGCTGACTCCGGCGGACGGGCCGCGCCTGCGCGCC
AGCGCATTTGCGCACAATCGCGGTCTGTCGCTGGCGCTGCGTCTGCTGGCGGAAACCTGCCCTCAGCTGGCGGATCTCGG
CGCGCCGGCCGCCTTAACGGAGCTGCTCAATGAAGAGAGCGGGCTTATCCTGGTCACGGGGGCTACCGGTAGCGGAAAAT
CAACCACCCTGGCGGCGATGGTGGGCTATCTTAACCAGCGCCTCAGCGGCCATATCCTGACGCTGGAAGATCCGGTTGAG
TTTATTCACCGCAGCGAGCGCTGTCTGATCCAGCAGCGGGAAATCGGCCGCCACTGCCCCTCATTTGCCGCTGCCCTACG
CGCCGCGCTGCGTCAGGACCCGGATGTCATTCTGTTAGGGGAGCTGCGTGACAGCGAAACGATCCGCCTGGCATTAACCG
CAGCGGAGACCGGACATCTGGTGATGGCAACGCTGCATACGCGCGGCGCAGCGCAGGCGGTTGAGAGGCTGGTGGACGTC
TTCCCGGCGCAGGAGAAAGAGCAGGTTCGCAGCCAACTGGCGGGGAGCTTATGCGCGGTGCTGGCACAAAAACTGTTTCC
AGATGCTGCTGGCGGCAGAGTTGCGTTATACGAGCTTTTGGTTAACACCCCGGCGGTGGCTAATTTGATCCGAGAGGGGA
AAACGCATCAGTTGCCCGGCGTGATGCAAACCGGGCAGCAATCGGGCATGCAAACATTTACGCAAAGCTTTCAGCAACGT
ACGGCCGCGGGGCTTTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A285B9N8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.544

100

0.5
  pilT Vibrio cholerae strain A1552

49.544

100

0.5
  pilT Neisseria meningitidis 8013

48.788

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.485

100

0.491
  pilT Legionella pneumophila strain Lp02

48.113

97.546

0.469
  pilT Legionella pneumophila strain ERS1305867

48.113

97.546

0.469
  pilT Pseudomonas stutzeri DSM 10701

45.897

100

0.463
  pilT Acinetobacter baumannii D1279779

45.455

100

0.46
  pilT Acinetobacter baumannii strain A118

45.455

100

0.46
  pilT Acinetobacter nosocomialis M2

45.152

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.289

100

0.457
  pilT Acinetobacter baylyi ADP1

44.985

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.795

97.239

0.436
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.414
  pilU Vibrio cholerae strain A1552

40.683

98.773

0.402
  pilU Acinetobacter baylyi ADP1

38.318

98.466

0.377