Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFJ24_RS03855 Genome accession   NZ_CP099389
Coordinates   792975..793955 (+) Length   326 a.a.
NCBI ID   WP_181636295.1    Uniprot ID   -
Organism   Citrobacter braakii strain RHB02-E4-C01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 787975..798955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFJ24_RS03825 (NFJ24_03820) - 788351..789337 (-) 987 WP_016157444.1 TRAP transporter substrate-binding protein -
  NFJ24_RS03830 (NFJ24_03825) hemW 789651..790787 (-) 1137 WP_235117292.1 radical SAM family heme chaperone HemW -
  NFJ24_RS03835 (NFJ24_03830) - 790780..791373 (-) 594 WP_016154402.1 XTP/dITP diphosphatase -
  NFJ24_RS03840 (NFJ24_03835) yggU 791381..791671 (-) 291 WP_005123286.1 DUF167 family protein YggU -
  NFJ24_RS03845 (NFJ24_03840) - 791668..792234 (-) 567 WP_016154401.1 YggT family protein -
  NFJ24_RS03850 (NFJ24_03845) - 792253..792957 (-) 705 WP_016154400.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFJ24_RS03855 (NFJ24_03850) pilT 792975..793955 (+) 981 WP_181636295.1 type IV pilus twitching motility protein PilT Machinery gene
  NFJ24_RS03860 (NFJ24_03855) ruvX 793952..794368 (-) 417 WP_003825428.1 Holliday junction resolvase RuvX -
  NFJ24_RS03865 (NFJ24_03860) - 794368..794931 (-) 564 WP_016154395.1 YqgE/AlgH family protein -
  NFJ24_RS03870 (NFJ24_03865) gshB 795108..796055 (-) 948 WP_016154394.1 glutathione synthase -
  NFJ24_RS03875 (NFJ24_03870) rsmE 796068..796799 (-) 732 WP_141875632.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFJ24_RS03880 (NFJ24_03875) endA 796875..797582 (-) 708 WP_016154392.1 deoxyribonuclease I -
  NFJ24_RS03885 (NFJ24_03880) - 797677..798174 (-) 498 WP_235117293.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35902.92 Da        Isoelectric Point: 6.1727

>NTDB_id=699376 NFJ24_RS03855 WP_181636295.1 792975..793955(+) (pilT) [Citrobacter braakii strain RHB02-E4-C01]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIGGKVETAPFTTPDVENLLMCWLSEQQQVQWREQGQIDFALTLADSRRLRA
SAFAHQQGTSLALRLLPLECPRLDDLQTPEAIPELLHGENGLVLVTGATGSGKSTTLAAMVEYLNQHIAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=699376 NFJ24_RS03855 WP_181636295.1 792975..793955(+) (pilT) [Citrobacter braakii strain RHB02-E4-C01]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATAGGTGGAAAAGTCGAAACCGCACCATTTACTACGCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGATTGATTTTGCCCTAACGCTGGCGGACTCCCGGCGTCTACGCGCC
AGCGCATTTGCTCATCAGCAGGGAACCTCGCTGGCGCTAAGATTGCTACCGCTTGAATGTCCTCGTTTAGACGATCTCCA
GACCCCCGAGGCCATACCTGAACTGCTGCACGGTGAAAATGGATTAGTTCTGGTCACTGGAGCCACCGGCAGCGGTAAAT
CAACCACCCTGGCGGCGATGGTGGAGTACCTTAATCAACATATTGCGGGGCATATTCTGACGCTGGAAGATCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAGCGGGAGGTGGGCGTACACTGCGCTTCTTTTGCTGCCGGTTTGCG
CGGCGCGTTACGCGAAGATCCCGACGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACTATTCGTTTGGCCTTAACGG
CAGCGGAGACCGGGCATCTGGTGTTGGCAACCTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGCTGGTGGACACC
TTTCCCGCTCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAGCTGCTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGTGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCAGCAGCAGCGC
CAGGCGCAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Neisseria meningitidis 8013

46.341

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.037

100

0.463
  pilT Legionella pneumophila strain ERS1305867

47.484

97.546

0.463
  pilT Legionella pneumophila strain Lp02

47.484

97.546

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.956

97.239

0.408
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362