Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFJ30_RS04395 Genome accession   NZ_CP099388
Coordinates   896382..897362 (+) Length   326 a.a.
NCBI ID   WP_169329842.1    Uniprot ID   -
Organism   Citrobacter braakii strain RHB03-E1-C02     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 891382..902362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFJ30_RS04365 (NFJ30_04360) - 891758..892744 (-) 987 WP_169329839.1 TRAP transporter substrate-binding protein -
  NFJ30_RS04370 (NFJ30_04365) hemW 893058..894194 (-) 1137 WP_169329840.1 radical SAM family heme chaperone HemW -
  NFJ30_RS04375 (NFJ30_04370) - 894187..894780 (-) 594 WP_019077664.1 XTP/dITP diphosphatase -
  NFJ30_RS04380 (NFJ30_04375) yggU 894788..895078 (-) 291 WP_005123286.1 DUF167 family protein YggU -
  NFJ30_RS04385 (NFJ30_04380) - 895075..895641 (-) 567 WP_016154401.1 YggT family protein -
  NFJ30_RS04390 (NFJ30_04385) - 895660..896364 (-) 705 WP_169329841.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFJ30_RS04395 (NFJ30_04390) pilT 896382..897362 (+) 981 WP_169329842.1 type IV pilus twitching motility protein PilT Machinery gene
  NFJ30_RS04400 (NFJ30_04395) ruvX 897359..897775 (-) 417 WP_003825428.1 Holliday junction resolvase RuvX -
  NFJ30_RS04405 (NFJ30_04400) - 897775..898338 (-) 564 WP_016154395.1 YqgE/AlgH family protein -
  NFJ30_RS04410 (NFJ30_04405) gshB 898515..899462 (-) 948 WP_016154394.1 glutathione synthase -
  NFJ30_RS04415 (NFJ30_04410) rsmE 899475..900206 (-) 732 WP_016154393.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFJ30_RS04420 (NFJ30_04415) endA 900282..900989 (-) 708 WP_101700211.1 deoxyribonuclease I -
  NFJ30_RS04425 (NFJ30_04420) - 901084..901581 (-) 498 WP_016157438.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36050.14 Da        Isoelectric Point: 6.4257

>NTDB_id=699353 NFJ30_RS04395 WP_169329842.1 896382..897362(+) (pilT) [Citrobacter braakii strain RHB03-E1-C02]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVEIAPFTPPDVENLLMCWLSEQQQVQWREQGQIDFALTLADSRRLRA
SAFAHQQGTSLALRLLPLECPRLDDLQTPEAIPELLHGENGLVLVTGATGSGKSTTLAAMVEYLNQHIAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTHAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=699353 NFJ30_RS04395 WP_169329842.1 896382..897362(+) (pilT) [Citrobacter braakii strain RHB03-E1-C02]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCGGCGCG
CTGGCGCATACGTGGAAAAGTCGAAATAGCACCATTTACTCCCCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGATTGATTTTGCCCTTACGCTGGCGGACTCCCGGCGTCTACGCGCC
AGCGCATTTGCTCATCAGCAGGGCACCTCGCTGGCGCTAAGATTGCTACCGCTTGAATGTCCTCGTTTAGACGATCTTCA
GACTCCGGAGGCCATACCTGAACTGCTGCACGGTGAAAATGGATTAGTTCTGGTCACTGGAGCCACCGGCAGCGGTAAAT
CAACCACCCTGGCGGCGATGGTGGAGTACCTTAATCAACATATTGCGGGGCATATTCTGACGCTGGAAGATCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAGCGGGAGGTGGGCGTACACTGCGCTTCTTTTGCTGCCGGTTTGCG
CGGCGCGTTACGCGAAGATCCCGACGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACTATTCGTTTGGCCTTAACGG
CAGCGGAGACCGGGCATCTGGTGTTGGCAACCTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGCTGGTGGACACC
TTTCCCGCTCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAACTGCTCGTCAACACTCACGCCGTGGGCAATTTGATCCGTGAAGGGA
AAACGCATCAGCTACCCGGTGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCAGCAGCAGCGC
CAGGCGCAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Neisseria meningitidis 8013

46.037

100

0.463
  pilT Legionella pneumophila strain Lp02

47.484

97.546

0.463
  pilT Legionella pneumophila strain ERS1305867

47.484

97.546

0.463
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Neisseria gonorrhoeae MS11

45.732

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.956

97.239

0.408
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Acinetobacter baylyi ADP1

36.42

99.387

0.362