Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFJ63_RS03840 Genome accession   NZ_CP099385
Coordinates   789065..790045 (+) Length   326 a.a.
NCBI ID   WP_103766651.1    Uniprot ID   -
Organism   Citrobacter braakii strain RHB09-SO-C07     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 784065..795045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFJ63_RS03795 (NFJ63_03800) hemW 784291..785427 (-) 1137 WP_103766649.1 radical SAM family heme chaperone HemW -
  NFJ63_RS03800 (NFJ63_03805) - 785420..786013 (-) 594 WP_016154402.1 XTP/dITP diphosphatase -
  NFJ63_RS03805 (NFJ63_03810) yggU 786021..786311 (-) 291 WP_005123286.1 DUF167 family protein YggU -
  NFJ63_RS03810 (NFJ63_03815) - 786308..786874 (-) 567 WP_016154401.1 YggT family protein -
  NFJ63_RS03815 (NFJ63_03820) - 786893..787597 (-) 705 WP_016154400.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFJ63_RS03820 (NFJ63_03825) - 787570..787740 (+) 171 Protein_748 type IV pili twitching motility protein PilT -
  NFJ63_RS03825 (NFJ63_03830) - 788093..788452 (+) 360 WP_103766650.1 hypothetical protein -
  NFJ63_RS03830 (NFJ63_03835) - 788452..788871 (+) 420 WP_049281521.1 DUF3828 domain-containing protein -
  NFJ63_RS03835 (NFJ63_03840) - 788877..789047 (-) 171 Protein_751 YggS family pyridoxal phosphate enzyme -
  NFJ63_RS03840 (NFJ63_03845) pilT 789065..790045 (+) 981 WP_103766651.1 type IV pilus twitching motility protein PilT Machinery gene
  NFJ63_RS03845 (NFJ63_03850) ruvX 790042..790458 (-) 417 WP_003825428.1 Holliday junction resolvase RuvX -
  NFJ63_RS03850 (NFJ63_03855) - 790458..791021 (-) 564 WP_016154395.1 YqgE/AlgH family protein -
  NFJ63_RS03855 (NFJ63_03860) gshB 791198..792145 (-) 948 WP_016154394.1 glutathione synthase -
  NFJ63_RS03860 (NFJ63_03865) rsmE 792158..792889 (-) 732 WP_016154393.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFJ63_RS03865 (NFJ63_03870) endA 792965..793672 (-) 708 WP_016154392.1 deoxyribonuclease I -
  NFJ63_RS03870 (NFJ63_03875) - 793767..794264 (-) 498 WP_016157438.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35910.04 Da        Isoelectric Point: 6.4971

>NTDB_id=699286 NFJ63_RS03840 WP_103766651.1 789065..790045(+) (pilT) [Citrobacter braakii strain RHB09-SO-C07]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIGGKVEIAPFTPPDVENLLMCWLSEQQQVQWREQGQIDFALTLADSRRLRA
SAFAHQQGTSLALRLLPLECPRLDDLQTPEAIPKLLHGENGLVLVTGATGSGKSTTLAAMVEYLNQHIAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=699286 NFJ63_RS03840 WP_103766651.1 789065..790045(+) (pilT) [Citrobacter braakii strain RHB09-SO-C07]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATAGGTGGAAAAGTCGAAATAGCACCATTTACTCCCCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGATTGATTTTGCCCTTACGCTGGCGGACTCCCGGCGTCTACGCGCC
AGCGCATTTGCTCATCAGCAGGGAACCTCGCTGGCGCTAAGATTGCTACCGCTTGAATGTCCTCGTTTAGACGATCTTCA
GACCCCCGAGGCCATACCTAAACTGCTGCACGGTGAAAATGGATTAGTTCTGGTCACTGGAGCCACCGGCAGCGGTAAAT
CAACTACCCTGGCGGCGATGGTGGAGTACCTTAATCAACATATTGCGGGGCACATTCTGACGCTGGAAGATCCGATTGAA
TATCGCTACACCAGCCAACGTTGTCTTATTCAACAGCGGGAGGTGGGCGTACACTGCGCTTCTTTTGCTGCCGGTTTGCG
CGGCGCGTTACGCGAAGATCCCGACGTTATTTTGCTGGGCGAGCTGCGCGACGTGGAAACTATTCGTTTGGCCTTAACGG
CAGCGGAGACCGGGCATCTGGTGTTGGCAACCTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGCTGGTGGACACC
TTTCCCGCTCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGTTGGAGGA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAACTGCTCGTCAACACGCCCGCCGTGGGTAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGTGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCAGCAGCAGCGC
CAGGCGCAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(59-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.07

100

0.512
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.07

100

0.512
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

47.17

97.546

0.46
  pilT Legionella pneumophila strain Lp02

47.17

97.546

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.271

97.239

0.411
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.42

99.387

0.362